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TheDynamicsofThrombinFormation
...olvedintheformationofthrombinleadstotheconclusionthatthephysiologicallyrelevanthemostaticmechanismiscomposedof3procoagulantvitaminK–dependentenzymecomplexes(whichusetheproteasesfactorIXa,factorXa,andfactorVIIa)andoneanticoagulantvitaminK–dependentcomplex.1EachcomplexinvolvesavitaminK–dependent...
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MechanismofActionofHigh-DoseFactorVIIa
...actorpathwaytothrombin,I:Anempiricalstudy.JBiolChem.1994;269:23357–23366.van’tVeerC,GoldenNJ,KalafatisM,MannKG.InhibitorymechanismoftheproteinCpathwayontissuefactor-inducedthrombingeneration:synergisticeffectincombinationwithtissuefactorpathwayinhibitor.JBiolChem.1997;272:7983–7994.ButenasS,va...
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鼻内镜下鼻腔泪囊造孔术的经验和体会
...三一爱尔眼科医院成功开展的鼻内镜下鼻腔泪囊造孔术(NES-DCR)6~12个月能随访到的病例178例192眼,现将其中的经验和体会总结如下。 1对象与方法 1.1对象本组178例(192眼)患者,男31例,女147例,年龄18~79,平均45.6岁。其...
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ASingleDecoyOligodeoxynucleotidesTargetingMultipleOncoproteinsProducesStrongAnticancerEffects
...t3concomitantly.WeevaluatedeffectsofthiscdODNonexpressionofcancer-relatedgenes,viabilityofhumancancercelllines,andinvivotumorgrowthinnudemice.ThecdODNtargetingallNF-B,E2F,andStat3togetherdemonstratedenhancementofefficacyofmorethan2-foldandincreasesinpotencyof2ordersofmagnitudecomparedwitheachofthedO...
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恶性嗜铬细胞瘤相关标记物的研究进展
...素瘤、嗜铬细胞瘤、神经母细胞瘤等的诊断和鉴别诊断。Van-der-Harst在评估嗜铬细胞瘤的潜在恶性时,研究了S-100阳性支持细胞的密度,发现S-100阳性支持细胞的缺失与恶性嗜铬细胞瘤显著相关(P0.0005),因此说明S-100阳性支持细...
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酶免疫分析及酶联免疫吸附实验
...典斯德哥尔摩大学的Perlmann,Engvall教授以及荷兰的Schuurs和van-Weemen教授独立而同时创造完成的。当今,在全球医学实验室里广泛应用的全自动分析仪每日可以检测大量的病人标本,其中很多仪器都是运用酶标记的免疫分析技术为检...
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396株嗜麦芽窄食单胞菌的耐药特征研究
...麦芽窄食单胞菌阳性后,其中1例停用IMP,另1例继续用IMP+VAN治疗,结果均死于原发病,未能复查血培养。表3 10例嗜麦芽窄食单胞菌菌血症治疗结果 Table3.Theanalysisof10casesofSMAbacterimiaAccessionSpecialPrimarydiseaseIntravenous catheterTherapie...
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ApoA-V
...iedwhethercholesterolisaphysiologicalligandforROR.TheconnectionbetweenapoA-VandRORopensupinterestingpossibilities,becauseRORhasbeenshowntopreventatherosclerosis(seeBoukhtoucheetal7forreview).Thus,boththeknockoutmiceandthenaturalknockout,theso-calledstaggerermice,haveanincreasedfrequencyofatheroscler...
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EvaluationofaFullyAutomatedSystem(RAISUS)forRapidIdentificationandAntimicrobialSusceptibilityTestingofStaphylococci
...esultsfromsusceptibilitytesting.Ifresistantbacteria(methicillin-resistantorvancomycin-resistantbacteria)couldbeidentifiedatanearlierstage,theuseofineffectiveantibioticscouldbeavoided,andachoicecouldbemadeofthemostsuitableantibacterialagentsforpatientswithinfectionscausedbyagivenbacterium.Moreover,im...
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生物物理所揭示自噬基因参与学习记忆功能的调节
Nes-Wdr45fl/Y小鼠出现学习记忆功能障碍和轴突水肿通过线虫遗传筛选的方法,中国科学院生物物理研究所张宏课题组的早期工作发现,epg-6基因是一个重要的多细胞生物特有的自噬基因。epg-6基因在哺乳动物中的同源基因为WDR45/WIPI...
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朊病毒蛋白113-120序列多肽的反相液相色谱保留行为与构象稳定性的关系
...发现在以乙腈溶液为流动相时,lnKw随温度的变化关系和Van′tHoff曲线均比较简单,说明该多肽在乙腈溶液中所采取的构象均较稳定,不易受温度的影响。以甲醇溶液为流动相时,具有游离末端的多肽的lnKw随温度变化关系和Van′tH...
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骨盆骨折治疗进展
...侧方挤压力可由暴力直接作用在髂骨上而造成半骨盆向内旋转或所谓“桶柄式骨折。垂直剪力为纵向暴力,可造成骨盆的纵向明显移位和广泛重要韧带的破坏。外旋力造成的“开书型损伤在外旋位是不稳定的,而内旋力或...
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DifferentialGeneExpressionofBlood-DerivedCellLinesinFamilialCombinedHyperlipidemia
...ertrophiccardiomyopathicend-stageheartfailure.PhysiolGenom.2002;10:31–44.vandeVijverMJ,HeYD,van’tVeerLJ,DaiH,HartAA,VoskuilDW,SchreiberGJ,PeterseJL,RobertsC,MartonMJ,ParrishM,AtsmaD,WitteveenA,GlasA,DelahayeL,vanderVeldeT,BartelinkH,RodenhuisS,RutgersET,FriendSH,BernardsR.Agene-expressionsignatu...
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老年股骨转子间骨折的DHS治疗
...下转子向股骨头颈部平行拧入一枚松质骨螺钉,加强共抗旋转能力[1]。 3.4手术时机对于老年人,适应证明确,且术前检查无禁忌证,全身情况允许的情况下,应尽快手术,争取在72h内使骨折获得固定。如果等待较长...
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Intakeofconjugatedlinoleicacid,fat,andotherfattyacidsinrelationtopostmenopausalbreastcancer:theNetherlandsCohortStudyonDietandCancer
LauraEVoorrips,HennyAMBrants,AlwineFMKardinaal,GerritJHiddink,PietAvandenBrandtandRAlexandraGoldbohm1FromtheDepartmentsofNutritionalEpidemiology(LEV,HAMB,andRAG)andNutritionalPhysiology(AFMK),TNONutritionandFoodResearch,Zeist,Netherlands;theDutchDairyFoundationforNutritionandHealth,Utrecht,Netherlan...
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PlantsterolintakesandcolorectalcancerriskintheNetherlandsCohortStudyonDietandCancer
ALenaNormén1,HennyAMBrants1,LauraEVoorrips1,HenrikAAndersson1,PietAvandenBrandt1andRAlexandraGoldbohm11FromtheDepartmentofClinicalNutrition,GöteborgUniversity,Göteborg,Sweden;theDepartmentofNutritionalEpidemiology,TNONutritionandFoodResearchInstitute,Zeist,Netherlands;andtheDepartme...
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细菌生理状态对中药抑菌活性的影响
...用传统的水煎法,分别称重、煎煮2次,混合煎出液,用旋转蒸发仪(70℃)将其浓缩为1g/ml,8磅20min灭菌后-20℃冷冻备用。1.4固体平板法测定中药的抑菌效果[2]配置一系列含不同药物浓度的LB固体平板。将指数生长期的细菌悬...
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InfluenceofFactorVIIaandPhospholipidsonCoagulationin“Acquired“Hemophilia
...ntheticinvitrocoagulationmodelssuggestthatfactorVIIaatpharmacologicallyrelevantconcentrationsisnotabletorestoreeitherthrombingenerationorsolidclotformationandthatTFisrequiredforanyprocoagulanteffect.20Thereactionsofthepropagationphaseofthebloodcoagulationcascadeleadingtothrombinformationinvivooccurp...
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隆突性皮肤纤维肉瘤临床病理及预后因素的研究
...包埋切片,HE染色,部分做网状纤维染色,Alican蓝染色和Van-Gieson染色,光镜下观察。患者年龄1.5~71岁,病程1~40年。发生部位:腹壁11例,胸壁3例,背部3例,上肢3例,大腿4例,膝下1例。2结果2.1病理检查(1)大体观察:肿...
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三味中药对解脲支原体体外抑制作用的实验研究
...然后注入已经融化的冷却至45℃的Uu琼脂培养液9ml,立即旋转混匀,凝固。分别取已传代3次的71株实验菌株各10μl接种于含有不同浓度三种中草药的琼脂平板(每个平板划10区,分别接种10株待检Uu菌株),生理盐水作为对照。置于...
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Patientinformationlettersonnutrition:developmentandimplementation
JaapJvanBinsbergenandAntonJMDrenthen1FromtheDepartmentofFamilyMedicine,UniversityMedicalCenterNijmegen,Nijmegen,theNetherlands(JJvB);andDutchCollegeofGeneralPractitioners,Utrecht,theNetherlands(AJMD).2PresentedattheThirdHeelsumInternationalWorkshop,heldinHeelsum,theNetherlands,December1012,200...
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APerspectiveonDNAMicroarraysinPathologyResearchandPractice
...rstandhumandisease.Therehasalsobeengreatexpectationthatthetechnologywouldadvancethepracticeofpathology.ThisreviewhighlightssomeofthekeycontributionsofDNAmicroarraystoexperimentalpathology,focusingintheareaofcancerresearch.Alsodiscussedaresomeofthecurrentchallengesintranslatingutilitytoclinicalpracti...
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肠球菌耐药性与耐万古霉素基因检测
...青霉素、氨苄西林均敏感,PCR检测结果只有粪肠球菌B144vanC2/3阳性,其余菌株van基因均为阴性。可见耐万古霉素肠球菌国内仍处于低水平,且没有发现具有临床意义的vanA、vanB型耐药,万古霉素、替考拉宁、青霉素、氨苄西林均...
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肝移植术后感染病原菌耐药性分析
...基噻吩显色法检测肠球菌的β-内酰胺酶;琼脂稀释法检测Van基因。结果55例患者肝移植术后感染主要为革兰氏阴性杆菌,其对4种及4种以上抗生素的耐药率均超过40%。阴沟肠杆菌与大肠杆菌的ESBLs与AmpC酶检出率分别为32.4%、36.8%和3...
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BreastCancerResistanceProtein(Bcrp1/Abcg2)LimitsNetIntestinalUptakeofQuercetininRatsbyFacilitatingApicalEffluxofGlucuronides
...toncancerandparticularlycardiovasculardiseasesisreported(LawandMorris,1998;van‘tVeeretal.,2000;ArtsandHollman,2005).Inexperimentalstudies,flavonoidshavebeenshowntobeneficiallyaffectseveralaspectsofcarcinogenesisandthedevelopmentofcardiovasculardiseases(Middletonetal.,2000).Uptakeofflavonoidsfromth...
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CultivationofNeisseriagonorrhoeaeinLiquidMediaandDeterminationofItsInVitroSusceptibilitiestoQuinolones
....Thesusceptibilitiesoflaboratory-adaptedstrainsandclinicalisolatestoquinolonesweretestedbythemicrodilutionmethodusingFB.TheMICsdeterminedbymicrodilutionwerenotsignificantlydifferentfromthosedeterminedbytheagardilutionmethodrecommendedbytheCLSI(formerlyNationalCommitteeforClinicalLaboratoryStandards)...
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EvaluationofaNewMolecularSystemforSimultaneousIdentificationofFourEnterococcusSpeciesandTheirGlycopeptideResistanceGenotypes
...EK2systemforidentificationof105enterococcalstrainsanddifferentiationoftheirvangene-associatedresistancelevels.Bothmethodsprovidedexcellentresults.Themolecularassayshowedadvantagesintimetoresultforidentificationofvan-associatedgenes.TEXTEnterococciplayanimportantroleinnosocomialinfections,e.g.,urinar...
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嗜酸细胞性膀胱炎2例报告并文献复习
...年首次报道以来,陆续有文献报道,但大都为个案报道。Van等[1]报道135例,发病年龄5d-87岁,平均年龄41.6岁,显示EC可发于任何年龄,男女发病无明显差异,儿童男性稍高于女性。由于EC诊断往往需膀胱镜检明确,其发病率往往...
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Controlledtrialofeffectofcomputer-basednutritioncourseonknowledgeandpracticeofgeneralpractitionertrainees
...Maiburg,Jan-JoostERethans,LambertWTSchuwirth,LisbethMHMathus-VliegenandJanWvanRee1FromtheDepartmentofGeneralPractice(HJSMandJWVR),theSkillslabDepartment(J-JER),andtheDepartmentofEducationalDevelopmentandResearch(LWTS),MaastrichtUniversity,Maastricht,theNetherlands;andtheDepartmentofGastroenterology,...
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ImmunesensingofCandidaalbicansrequirescooperativerecognitionofmannansandglucansbylectinandToll-likereceptors
...onsinUnitedStateshospitals:athree-yearanalysis.Clin.Infect.Dis.29::239-244.Van‘tWout,J.W.,Linde,I.,Leijh,P.C.J.,andVanFurth,R.1988..ContributionofgranulocytesandmonocytestoresistanceagainstexperimentaldisseminatedCandidaalbicansinfections.Eur.J.Clin.Microbiol.Infect.Dis.7::736-741.Kullberg,B.J.,Va...