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冠心病与免疫
脂纹内皮下脂质过度堆积的巨噬细胞泡沫细胞,fattystreakT淋巴细胞10岁见于主动脉,20岁见于冠脉,30岁见于脑动脉发展早期病灶中心坏死,含细胞碎片、胆固醇、炎症细胞、巨噬细胞泡沫细胞,由平滑肌细胞、细胞外基质,内表...
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GeneticDiversityandRecombinationofPorcineSapoviruses
...manstrainsexist.INTRODUCTIONSapoviruses(SaV),previouslyreferredtoasSapporo-likeviruses,areemergingentericpathogensthatcausediarrheainhumans,pigs,andmink(5,7,9,10,22).Theyarenonenveloped,polyadenylatedsingle-strandedpositive-senseRNAvirusesandbelongtotheSapovirusgenusinthefamilyCaliciviridae(7).TheSa...
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肺炎患儿心肌损害205例分析
...2)缺氧、酸碱平衡失调及细菌、病毒等毒素均可致心肌细胞损害而引起ST-T改变、Q—T间期延长及各种异位搏动的发生;(3)缺氧导致肺动脉高压,使右心负荷加重,在心电图上可表现为P波高尖、不完全性右束支传导阻滞;(4)病...
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SensitiveEDTA-BasedMicrobiologicalAssaysforDetectionofMetallo--LactamasesinNonfermentativeGram-NegativeBacteria
...othecrudeextracts10minbeforeperformingtheassay.ThepresenceofthegenesblaVIM-like,blaIMP-like,andblaSPM-1(forbothPseudomonasspp.andA.baumannii)andblaL1-like,blaIND-like,andblaGOB-like(forStenotrophomonasmaltophilia,Chryseobacteriumindologenes,andChryseobacteriummeningosepticum,respectively)wasanalyzed...
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一种新型的polo-like激酶途径调节剂ON01910.Na在成人实体瘤治疗的I期临床试验
SOURCE:JournalofClinicalOncology一种新型的polo-like激酶途径调节剂ON01910.Na对成人实体瘤患者进行了I期临床试验,旨在确定其剂量限制性毒性(dose-limitingtoxicities,DLTs)。20例(11女,9男)难治性(针对常规治疗)实体瘤患者纳入研究,...
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单链抗体的研究进展及在肿瘤诊断、治疗中的应用
自从1975年K?hler和Milstein创立淋巴细胞杂交瘤技术以来,单克隆抗体技术在临床诊断和基础研究中的应用越来越广泛,但在疾病治疗方面,还没有达到令人满意的效果,原因主要是应用的单克隆抗体绝大多数来自鼠类,而鼠抗体在...
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VacciniavirusproteinA46RtargetsmultipleToll-like–interleukin-1receptoradaptorsandcontributestovirulence
...tkeyaspectsofthehostantiviralresponse.Recently,ithasbeenrecognizedthatToll-likereceptors(TLRs)havearoleininnatedefenseagainstviruses.Here,wedefinethefunctionofthevacciniavirus(VV)proteinA46RandshowitinhibitsintracellularsignallingbyarangeofTLRs.TLRsignallingistriggeredbyhomotypicinteractionsbetweent...
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DevelopmentofaMultiplexPCRandSHVMelting-CurveMutationDetectionSystemforDetectionofSomeSHVandCTX-M-LactamasesofEscherichiacoli,Klebsiella
...mentofanovelsystem,whichconsistsofamultiplexPCRtoidentifyblaSHV,blaCTX-M-3-like,andblaCTX-M-14-likegenesandamodifiedSHVmelting-curvemutationdetectionmethodtorapidlydistinguishsixprevalentblaSHVgenes(blaSHV-1,blaSHV-2,blaSHV-2a,blaSHV-5,blaSHV-11,andblaSHV-12)inTaiwan.Sixty-fiveclinicalisolates,which...
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DefinitionoftargetantigensfornaturallyoccurringCD4+CD25+regulatoryTcells
...dsencodingthefollowingSEREX-definedself-antigens:MusheatshockproteinDnaJ–like2(DnaJ–like2;AF055664),MusDNAligase1(Ligase1;U19604),Musgalectin-8(Galectin-8;AF218069),orMuspoly(A)bindingprotein,cytoplasmic1(Poly(A);X65553).1wkafterimmunization,splenicCD4+CD25+TcellsandCD4+CD25–Tcellswereisolated...
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MolecularEpidemiologyofNorovirusInfectionsinStockholm,Sweden,duringtheYears2000to2003:AssociationoftheGGIIbGeneticClusterwithInfectionin
...evel(genogroupII).Twenty-nineoutof39(74%)oftheRLB-typedspecimenswereGGII-4-like.TheGGII-positivespecimensthatcouldnotbefurthertypedwerefrom36childrenand11adultpatients.Only5outof41specimensfromchildrenmatchedtheRLBpanel,andofthose,4wereGGII-4-likeandonewasGGI-6-like.Thelastoriginatedfromafood-borneo...
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寻找药物设计的新靶点
...来有关研究的概况。 1 靶向CD分子的治疗制剂 白细胞分化抗原(CD分子)是白细胞在正常分化过程的不同阶段出现或消失的细胞表面分子,它们大多是蛋白质或糖蛋白,一般可分为胞外区、穿膜区和胞内区。目前CD分子共有...
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2000级医学免疫学期末考试试题
...补体裂解片段C3a、C5a具有趋化作用和____________作用。4.T细胞识别的抗原决定簇是__________。5.补体经典激活途径的主要激活物质是__________。6.位于HLA-Ⅱ类基因区,其编码产物参与内源性抗原加工递呈的基因是LMP和____。7.根据...
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MolecularCharacterizationsofHumanandAnimalGroupARotavirusesinTheNetherlands
...peG8wasnotfound.GenotypeG6bovinerotavirusesweredividedintothreeclusters:UK-like,VMRI-29-like,andHun4-like.DNAsequencingofapartoftheVP7genewasshowntobeusefulasaquickdeterminationofuncommonornovelstrainsofwhichthegenotypingcannotbedonebygenotypingPCR.Ofequinestrains,bothVP4andVP7genescouldbeusedforgen...
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毒力岛和细菌毒力的进化
...ol%、密码使用和宿主细菌染色体有明显差异,有的比宿主细胞的G+Cmol%明显高,有的明显低;5.毒力岛编码的基因产物许多是分泌性蛋白和细胞表面蛋白,如溶血素、菌毛和血红素结合因子,一些毒力岛编码细菌的分泌系统(如Ⅲ...
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放线共生放线杆菌的白细胞毒素
...周炎(LJP)的重要致病菌。在A.a的各种毒力因子中[1],白细胞毒素(Ltx)得到了广泛关注。不同的A.a株产生Ltx的能力不同。高毒力株JP2生成的Ltx是低毒力株652的10-20倍[1],这种显著的差异对A.a的致病力有着决定性意义[2]。牙周...
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超敏反应复习题
...A.组胺B.白三烯C.肝素D.腺苷酸环化酶E.前列腺素2.哪些细胞表达高亲和力的FcεRI?A.单核细胞、巨噬细胞B.中性粒细胞、肥大细胞C.中性粒细胞、嗜碱性粒细胞D.肥大细胞、嗜碱性粒细胞E.嗜酸性粒细胞、嗜碱性粒细胞3....
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肝硬化并反流性食管炎的临床、内镜及病理分析
...色检查,亦按1999年烟台标准进行分级。鳞状上皮增生、炎细胞浸润为轻度,出现黏膜糜烂为中度,有溃疡形成或Barrett食管改变为重度。 1.4统计学处理采用χ2检验。 2结果 2.1内镜检查结果RE内镜下表现为黏膜点状或条状...
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MolecularEpidemiologyofCalicivirusesDetectedinSporadicandOutbreakCasesofGastroenteritisinFrancefromDecember1998toFebruary2004
...hichhadnotbeendetectedbeforeinourlaboratory:thenewvariant,GGIIb,andNorwalk-likestrains,whichweredifficulttodetect,in2000aswellasanewvariantLordsdalestrainin2002.Althoughthesestrainsemergedduringaperiod,othergenotypesstillcocirculated.Amongthesethreegroupsofstrains,thenewvariant,GGIIb,andthenewLordsd...
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金黄色葡萄球菌肠毒素及其检测方法的研究进展
...ccalenterotoxins,SE)、葡萄球菌溶素、表皮剥脱毒素、杀白细胞素、毒性休克综合征毒素—1等;产生的主要酶类有:凝固酶、耐热核酸酶、葡激酶等,其中SE在该菌的致病性中起着重要的作用。在美国由SE引起的食物中毒占整个...
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NucleicacidsofmammalianorigincanactasendogenousligandsforToll-likereceptorsandmaypromotesystemiclupuserythematosus
...FN-.Wehavedevelopedthreeseriesofoligonucleotide(ODN)-basedinhibitorsofToll-likereceptor(TLR)signaling.TheseODNsincludeinhibitorsofTLR9,inhibitorsofTLR7butnotTLR9,andsequencesthatinhibitbothTLR7andTLR9.SpecificityoftheseinhibitorsisconfirmedbyinhibitionofIFN-productionbyPDCsinresponsetoDNAorRNAviruse...
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肠出血性大肠埃希氏菌的多重PCR检测方法
...医学细菌学重点实验室)关键词:大肠杆菌;溶血素类;毒素类;聚合酶链反应 【摘要】 目的 开发肠出血性大肠埃希氏菌(entero-hemorrhagicE.coli,EHEC)的多重PCR检测方法。方法 以溶血素(hemolysin,hly)基因和志贺样毒素(Shigaliket...
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Community-WideOutbreaksofClonallyRelatedCTX-M-14-Lactamase-ProducingEscherichiacoliStrainsintheCalgaryHealthRegion
...57(15%)ofthestrainswerepositiveforblaCTX-MgenesfromtheCTX-M-Igroup(CTX-M-1-like)and87of157(55%)ofthestrainswerepositiveforblaCTX-MgenesfromtheCTX-M-IIIgroup(CTX-M-14-like).Theobjectiveofthisstudywastoinvestigatethemolecularepidemiologyofthesestrains.The-lactamaseswerecharacterized,andthegeneticrelat...
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慢性HBV感染免疫治疗研究进展
...称DNA疫苗,它不仅可诱导体液免疫应答,而且能诱导强烈的细胞免疫应答,有望用于病毒性肝炎的治疗。JungM-C等应用前S1、前S2和S抗原组分疫苗,能够引发慢性HBV携带者针对HBsAg的CD4+T淋巴细胞应答,但未能介导Thl细胞因子分泌和病毒特...
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生物农药的发展动态与趋势展望
...和黄瓜花叶病毒的转基因烟草、抗矮缩病毒水稻等。 细胞工程学使药源植物如印楝、鱼藤等的细胞离体培养取得突破;生物技术如酶联免疫分析技术用来检测有机化学农药的残留,反过来又促进生物农药的发展;抗除草剂的农...
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Interleukin-1receptor-associatedkinase-1playsanessentialroleforToll-likereceptor(TLR)7-andTLR9-mediatedinterferon-induction
...amonMicrobiologyandImmunology,OsakaUniversity,SuitaOsaka565-0871,JapanToll-likereceptors(TLRs)recognizemicrobialpathogensandtriggerinnateimmuneresponses.AmongTLRfamilymembers,TLR7,TLR8,andTLR9induceinterferon(IFN)-inplasmacytoiddendriticcells(pDCs).Thisinductionrequirestheformationofacomplexconsisti...
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Oxidativestressinsepsis:aredoxredux
...yD88;(b)TIRdomain–containingadaptorinducingIFN-?(TRIF);(c)MyD88adaptor–like/TIR-associatedprotein(MAL/TIRAP);and(d)TRIF-relatedadaptormolecule(TRAM)(2).InthecaseofLPSsignalingthroughTLR4,theMyD88-dependentpathwayiscriticalforNF-BactivationandtheproductionofTNF-whereastheMyD88-independent,TRIF-de...
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核因子κB的结构和功能与内毒素介导的肝损伤的关系
...ppaB(NF-κB)是近年来研究极为广泛的核内转录调控因子,在细胞炎性反应、免疫反应以及细胞凋亡等过程中起着举足轻重的作用。NF-κB参与多种细胞因子及急性期反应蛋白转录活性的调控,在免疫和炎性疾病的启动中起重要作用。...
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Humanskincellssupportthymus-independentTcelldevelopment
...opment(18)aswellasmanyofthesamekeratinsasthymicepithelialcells(19)anddelta-likeNotchligands(20).Hassallcorpuscles,aproductofmedullarythymicepithelialcells,containkeratinsidenticaltothoseseeninthestratumcorneumofskin(19).Similarly,thymicstromalcellsbearastrongresemblancetothefibroblastsofskin.Infact,...
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超抗原SEB
【摘要】金黄色葡萄球菌肠毒素B型(SEB)是一种典型的T细胞性超抗原,可引起T细胞多克隆活化。本文综述了SEB的结构和功能,并重点介绍了SEB类毒素的超抗原性。 【关键词】超抗原;SEB;SEB类毒素 超抗原(superantigen)...
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幽门螺杆菌菌苗及免疫相关问题研究进展
...理包括Hp的尿素酶、蛋白酶、脂酶、磷脂酶A、趋化因子及细胞空泡毒素(VacA)等直接致病,也包括Hp引起宿主的炎症及免疫应答致病[1]。本文主要就Hp菌苗与宿主的免疫应答及相关问题的研究进展作一简要综述。 hp与菌苗研...