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肝硬化并反流性食管炎的临床、内镜及病理分析
...色检查,亦按1999年烟台标准进行分级。鳞状上皮增生、炎细胞浸润为轻度,出现黏膜糜烂为中度,有溃疡形成或Barrett食管改变为重度。 1.4统计学处理采用χ2检验。 2结果 2.1内镜检查结果RE内镜下表现为黏膜点状或条状...
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核因子κB的结构和功能与内毒素介导的肝损伤的关系
...ppaB(NF-κB)是近年来研究极为广泛的核内转录调控因子,在细胞炎性反应、免疫反应以及细胞凋亡等过程中起着举足轻重的作用。NF-κB参与多种细胞因子及急性期反应蛋白转录活性的调控,在免疫和炎性疾病的启动中起重要作用。...
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GeneticDiversityandRecombinationofPorcineSapoviruses
...manstrainsexist.INTRODUCTIONSapoviruses(SaV),previouslyreferredtoasSapporo-likeviruses,areemergingentericpathogensthatcausediarrheainhumans,pigs,andmink(5,7,9,10,22).Theyarenonenveloped,polyadenylatedsingle-strandedpositive-senseRNAvirusesandbelongtotheSapovirusgenusinthefamilyCaliciviridae(7).TheSa...
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SensitiveEDTA-BasedMicrobiologicalAssaysforDetectionofMetallo--LactamasesinNonfermentativeGram-NegativeBacteria
...othecrudeextracts10minbeforeperformingtheassay.ThepresenceofthegenesblaVIM-like,blaIMP-like,andblaSPM-1(forbothPseudomonasspp.andA.baumannii)andblaL1-like,blaIND-like,andblaGOB-like(forStenotrophomonasmaltophilia,Chryseobacteriumindologenes,andChryseobacteriummeningosepticum,respectively)wasanalyzed...
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一种新型的polo-like激酶途径调节剂ON01910.Na在成人实体瘤治疗的I期临床试验
SOURCE:JournalofClinicalOncology一种新型的polo-like激酶途径调节剂ON01910.Na对成人实体瘤患者进行了I期临床试验,旨在确定其剂量限制性毒性(dose-limitingtoxicities,DLTs)。20例(11女,9男)难治性(针对常规治疗)实体瘤患者纳入研究,...
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肺炎患儿心肌损害205例分析
...2)缺氧、酸碱平衡失调及细菌、病毒等毒素均可致心肌细胞损害而引起ST-T改变、Q—T间期延长及各种异位搏动的发生;(3)缺氧导致肺动脉高压,使右心负荷加重,在心电图上可表现为P波高尖、不完全性右束支传导阻滞;(4)病...
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DevelopmentofaMultiplexPCRandSHVMelting-CurveMutationDetectionSystemforDetectionofSomeSHVandCTX-M-LactamasesofEscherichiacoli,Klebsiella
...mentofanovelsystem,whichconsistsofamultiplexPCRtoidentifyblaSHV,blaCTX-M-3-like,andblaCTX-M-14-likegenesandamodifiedSHVmelting-curvemutationdetectionmethodtorapidlydistinguishsixprevalentblaSHVgenes(blaSHV-1,blaSHV-2,blaSHV-2a,blaSHV-5,blaSHV-11,andblaSHV-12)inTaiwan.Sixty-fiveclinicalisolates,which...
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内毒素激活巨噬细胞的信号传导
...通过与膜受体CD14作用将信号从胞外传到胞核而刺激多种细胞合成和释放TNF-α、IL-1和IL-6等生物活性物质而表现复杂的生物学活性,但其详细机制尚未完全清楚。本文就近年来对LPS激活巨噬细胞的信号传导机制的研究作一简要综...
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VacciniavirusproteinA46RtargetsmultipleToll-like–interleukin-1receptoradaptorsandcontributestovirulence
...tkeyaspectsofthehostantiviralresponse.Recently,ithasbeenrecognizedthatToll-likereceptors(TLRs)havearoleininnatedefenseagainstviruses.Here,wedefinethefunctionofthevacciniavirus(VV)proteinA46RandshowitinhibitsintracellularsignallingbyarangeofTLRs.TLRsignallingistriggeredbyhomotypicinteractionsbetweent...
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MolecularCharacterizationsofHumanandAnimalGroupARotavirusesinTheNetherlands
...peG8wasnotfound.GenotypeG6bovinerotavirusesweredividedintothreeclusters:UK-like,VMRI-29-like,andHun4-like.DNAsequencingofapartoftheVP7genewasshowntobeusefulasaquickdeterminationofuncommonornovelstrainsofwhichthegenotypingcannotbedonebygenotypingPCR.Ofequinestrains,bothVP4andVP7genescouldbeusedforgen...
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补体系统复习题
...A.C2aB.C3aC.C3bD.C5aE.Ba6.补体不具备下列哪种作用?A.溶菌和细胞毒作用B.中和毒素作用C.调理作用D.炎症介质作用E.免疫调节作用7.具有趋化作用的补体组分是A.C2aB.C3bC.BaD.C4aE.C5a8.具有免疫粘附作用的物质A.C2aB.C2bC.C3aD.C3bE.C4a9.补体经典...
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SerumAmyloidAInducesContraryImmuneResponsesviaFormylPeptideReceptor-Like1inHumanMonocytes
...rTNF-andIL-10production,respectively.Thestimulationofformylpeptidereceptor-like-1-expressingRBL-2H3cells,butnotofvector-expressingRBL-2H3cellswithserumamyloidA,inducedmitogen-activatedproteinkinasesactivationandtheaccumulationoftheRNAsofthesetwocytokines.Together,ourfindingssuggestthatserumamyloidAm...
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Opioidreceptor-like1stimulationinthecollectingductinducesaquaresisthroughvasopressin-independentaquaporin-2downregulation
...ociceptin,theendogenousligandoftheinhibitoryGprotein-coupledopioidreceptor-like1receptor,producesaquaresis(i.e.,increasestheexcretionofsolute-freeurine)inrats.However,themechanismunderlyingthiseffecthasnotyetbeenexplained.Usingimmunohistochemistry,wefoundtheopioidreceptor-like1receptorintheratkidney...
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Community-WideOutbreaksofClonallyRelatedCTX-M-14-Lactamase-ProducingEscherichiacoliStrainsintheCalgaryHealthRegion
...57(15%)ofthestrainswerepositiveforblaCTX-MgenesfromtheCTX-M-Igroup(CTX-M-1-like)and87of157(55%)ofthestrainswerepositiveforblaCTX-MgenesfromtheCTX-M-IIIgroup(CTX-M-14-like).Theobjectiveofthisstudywastoinvestigatethemolecularepidemiologyofthesestrains.The-lactamaseswerecharacterized,andthegeneticrelat...
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肠产志贺样毒素的大肠杆菌菌株中小肠结肠炎耶尔森菌毒力岛基因irp-2的检测
...进化的一个重要的推动力量〔1,2〕。另外,毒力岛和宿主细胞的G+CMol%、密码使用的明显差异,也提示毒力岛可能是外源性的。目前认为水平性的基因转移可能会导致新的病原性细菌的产生。毒力岛的发现,使这一学说得到了广...
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毒力岛和细菌毒力的进化
...ol%、密码使用和宿主细菌染色体有明显差异,有的比宿主细胞的G+Cmol%明显高,有的明显低;5.毒力岛编码的基因产物许多是分泌性蛋白和细胞表面蛋白,如溶血素、菌毛和血红素结合因子,一些毒力岛编码细菌的分泌系统(如Ⅲ...
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MolecularEpidemiologyofNorovirusInfectionsinStockholm,Sweden,duringtheYears2000to2003:AssociationoftheGGIIbGeneticClusterwithInfectionin
...evel(genogroupII).Twenty-nineoutof39(74%)oftheRLB-typedspecimenswereGGII-4-like.TheGGII-positivespecimensthatcouldnotbefurthertypedwerefrom36childrenand11adultpatients.Only5outof41specimensfromchildrenmatchedtheRLBpanel,andofthose,4wereGGII-4-likeandonewasGGI-6-like.Thelastoriginatedfromafood-borneo...
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肠出血性大肠埃希氏菌的多重PCR检测方法
...医学细菌学重点实验室)关键词:大肠杆菌;溶血素类;毒素类;聚合酶链反应 【摘要】 目的 开发肠出血性大肠埃希氏菌(entero-hemorrhagicE.coli,EHEC)的多重PCR检测方法。方法 以溶血素(hemolysin,hly)基因和志贺样毒素(Shigaliket...
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致泻性大肠杆菌中发现小肠结肠炎耶尔森菌毒力岛
...类菌能同时与Inv、SLT1或SLT2探针杂交呈阳性反应,对Hep-2细胞呈集聚性粘附,但不与EAggEC特异性探针反应,且不具有EIEC及志贺氏菌的ipaB基因〔2〕。 近年来,在医学细菌学领域出现了一个新名词——毒力岛(pathogenicityisland)...
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AnIGF-Iantagonistdoesnotinhibitrenalfibrosisintheratfollowingsubtotalnephrectomy
...Biotechnology,UniversityofSheffield,Sheffield,UnitedKingdomABSTRACTInsulin-likegrowthfactorI(IGF-I)hasbeenproposedasamediatorofkidneyscarring,althoughnointerventionalstudiesontheroleofIGF-Iinmodelsofchronickidneydiseasehavebeenreported.TheeffectofapeptideIGF-Ireceptorantagonist(JB3)hasbeenexaminedon...
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MolecularEpidemiologyofCalicivirusesDetectedinSporadicandOutbreakCasesofGastroenteritisinFrancefromDecember1998toFebruary2004
...hichhadnotbeendetectedbeforeinourlaboratory:thenewvariant,GGIIb,andNorwalk-likestrains,whichweredifficulttodetect,in2000aswellasanewvariantLordsdalestrainin2002.Althoughthesestrainsemergedduringaperiod,othergenotypesstillcocirculated.Amongthesethreegroupsofstrains,thenewvariant,GGIIb,andthenewLordsd...
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寻找药物设计的新靶点
...来有关研究的概况。 1 靶向CD分子的治疗制剂 白细胞分化抗原(CD分子)是白细胞在正常分化过程的不同阶段出现或消失的细胞表面分子,它们大多是蛋白质或糖蛋白,一般可分为胞外区、穿膜区和胞内区。目前CD分子共有...
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NucleicacidsofmammalianorigincanactasendogenousligandsforToll-likereceptorsandmaypromotesystemiclupuserythematosus
...FN-.Wehavedevelopedthreeseriesofoligonucleotide(ODN)-basedinhibitorsofToll-likereceptor(TLR)signaling.TheseODNsincludeinhibitorsofTLR9,inhibitorsofTLR7butnotTLR9,andsequencesthatinhibitbothTLR7andTLR9.SpecificityoftheseinhibitorsisconfirmedbyinhibitionofIFN-productionbyPDCsinresponsetoDNAorRNAviruse...
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核因子-kappaB与急性肺损伤
...一具有多功能转录与调节作用的蛋白质因子,它能与多种细胞因子和粘附因子基因启动子部位发生结合,增强这些基因的转录和表达,还可能延缓PMN的凋亡,从而广泛参与基因转录的调节,特别是一些与机体免疫应答反映密切相...
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肠缺血再灌注损伤防治研究进展
...起网状内皮系统发生系列反应,进而导致大量相关介质和细胞因子的释放,甚至发生多系统器官功能不全综合征[1]。因此近年来越来越受到重视,其发生发展机理及防治措施的研究也成为外科领域的重点课题之一。 肠I/R...
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DefinitionoftargetantigensfornaturallyoccurringCD4+CD25+regulatoryTcells
...dsencodingthefollowingSEREX-definedself-antigens:MusheatshockproteinDnaJ–like2(DnaJ–like2;AF055664),MusDNAligase1(Ligase1;U19604),Musgalectin-8(Galectin-8;AF218069),orMuspoly(A)bindingprotein,cytoplasmic1(Poly(A);X65553).1wkafterimmunization,splenicCD4+CD25+TcellsandCD4+CD25–Tcellswereisolated...
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冠心病与免疫
脂纹内皮下脂质过度堆积的巨噬细胞泡沫细胞,fattystreakT淋巴细胞10岁见于主动脉,20岁见于冠脉,30岁见于脑动脉发展早期病灶中心坏死,含细胞碎片、胆固醇、炎症细胞、巨噬细胞泡沫细胞,由平滑肌细胞、细胞外基质,内表...
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TLR4易化细菌在肾集合管细胞中的迁移
...表了一篇文章,认为致肾盂肾炎大肠杆菌在肾髓质集合管细胞的移位和定植与TLR4易化的脂筏功能相关。致肾盂肾炎大肠杆菌(UropathogenicEscherichiacoli,UPEC)是最常见的尿路感染和肾盂肾炎的致病菌,肾髓质集合管细胞是UPEC定植...
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上海生科院通过单细胞RNA测序对躯体感觉神经元重新分类
图A.热图显示了197个神经元依据细胞大小排列的层次聚类的矩阵。10个族群通过黑线方框标注,族群的细胞大小显示在左侧。图B.背根神经节初级感觉神经元分类示意图。依据单细胞测序和原位杂交数据建议其类别和比例,依据电...
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TempolreducesoxidativestressandrestoresrenaldopamineD1-likereceptor-Gproteincouplingandfunctioninhyperglycemicrats
【摘要】DopamineviaactivationofrenalD1-likereceptorsinhibitstheactivitiesofNa-K-ATPaseandNa/Hexchangerandsubsequentlyincreasessodiumexcretion.Decreasedrenaldopamineproductionandsodiumexcretionareassociatedwithhyperglycemicconditions.WehaveearlierreportedD1-likereceptor-Gproteinuncouplingandreduc...