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磷酸甘油酸激酶的研究进展
...510089,Guangdong,PRChina)磷酸甘油酸激酶(PhosphoglyceratekinasePGK)是糖酵解的关键酶,也是每种生物得以生存的必须酶,该酶的缺乏可引起生物体代谢等功能的紊乱。PGK是一个单体的、高度柔曲性的糖酵解酶,它主要由两个球形的结...
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可溶性TNF受体在大肠杆菌中表达
...经XbalⅠ酶切、连接、转化大肠杆菌JM101,构成新的重组子pGK-sTNFR。重组质粒经XbalⅠ,TaqI酶切鉴定及采用双脱氧链终止法对sTNFR进行DNA序列分析表明,所克隆的sTNFR基因与报道的一致(见图1)。 图1 pGK-sTNFR...
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糖直接酵解径路中酶缺乏引起的溶血性贫血有哪些?
...值。本病由常染色体隐性遗传。 (8)磷酸甘油酸激酶(PGK)缺乏 PGK在1,3二磷酸甘油酸产生三磷酸腺苷(ATP)及三磷酸甘油酸产生二磷酸腺苷(ADP)的过程中均有作用,PGK缺乏时ATP的生成会明显减少,结果影响红细胞膜的功能。患...
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低氧环境影响小鼠未成熟关节透明软骨细胞培养
...-1(GLUT-1)、葡萄糖转运体-3(GLUT-3)、磷酸果糖激酶-1(PGK-1)和低氧诱导因子-1α(HIF—1α)的表达。应用定量PCR观察软骨细胞表型改变。应用四甲基偶氮唑盐法(MTT)检测低氧及氯化钴对软骨细胞活性的影响。用葡萄糖检测试...
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第二军医大Hepatology解析非编码RNA与癌症
...MVIH有可能通过抑制磷酸甘油酸激酶1(Phosphoglyceratekinase1,PGK1)促进了肿瘤诱导的血管生成。此外,在65个肝癌样本中,研究人员证实MVIH的表达与PGK1血清水平负相关,与微血管密度正相关。这些研究结果表明lncRNAMVIH失控可作为肝切...
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被癌细胞遗弃的激酶PKG
...组织(确保癌组织被全部清除)相比,PKG水平明显不同。PGK水平的变化究竟是手术造成的还是癌细胞存活的关键环节?许多蛋白都被癌细胞丢失了,那么将PGK重新放回癌细胞会有怎样后果呢?Browning首先向转移性结直肠癌细胞再...
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上海应物所等提出铝对人体生物毒性的新分子机制
...白的二级结构,使蛋白产生不可逆的变性和积聚。他们用PGK蛋白为例子,通过同步辐射能谱和XPS、CD、NMR等实验证明了这一点:1)CD实验显示,铝离子可以使PGK蛋白产生不可逆变性、积聚;2)同步辐射能谱和XPS实验表明,蛋白积...
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TargetingthelocaltumormicroenvironmentwithvacciniavirusexpressingB7.1forthetreatmentofmelanoma
...ld(CT)valuesofgeneexpressionfortheendogenouscontrolphosphoglyceratekinase1(PGK1)werecomparedtoruleoutdifferencesinaRNAassessmentduringprocessingpriortoqRT-PCRanalysis.Therewerenodifferencesfoundamongall5prevaccination(20.08±0.98)and16postvaccination(20.1±1.2)PGK1CTvaluesfrompatients3,4,5,7,and9.Am...
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南京大学首席科学家Cell子刊揭示表观遗传修饰酶的新功能
...调控开关)。去年,来自复旦大学的研究人员证实HDAC3是PGK1的去乙酰化酶。胰岛素及mTOR信号通路调控了HDAC3介导的PGK1去乙酰化及激活(延伸阅读:复旦管坤良、熊跃、叶丹联合发布表观遗传新成果)。在核心的哺乳动物昼夜节...
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TheImpactofHypoxicTreatmentontheExpressionofPhosphoglycerateKinaseandtheCytotoxicityofTroxacitabineandGemcitabine
...heamountofthetriphosphateform(L-OddCTP)innuclearDNA.Phosphoglyceratekinase(PGK),adownstreamproteinofhypoxia-inducible-factor-1(HIF-1),isresponsibleforthephosphorylationofthediphosphatetothetriphosphateofL-OddC.Inthisstudy,westudiedtheimpactofhypoxiaonthemetabolismandthecytotoxicityofL-OddCand-D-2‘...
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哪些红细胞酶缺乏可引起贫血?
...脱氢酶(PGD)、二磷酸甘油酸变位酶(DPGM)、磷酸甘油酸激酶(PGK)、丙酮酸激酶(PK)、三磷酸腺苷酶(ATPase); ②磷酸己糖旁路(HMP)中的酶:葡糖六磷酸脱氢酶(G6PD)、六磷酸葡糖酸脱氢酶(6PGD)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还...
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田世平研究组发现转录因子RIN调控果实成熟衰老分子机制
...化酶类似蛋白E8)、糖酵解相关基因(磷酸甘油激酶前体PGK)、次生代谢相关基因(聚精液素醛酯酶PNAE),以及芳香物质代谢相关基因(脂氧合酶LOX和乙醇脱氢酶ADH2)的表达,RIN蛋白能够与这些基因的启动子序列结合。RIN基因...
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青霉素裂解液的高效液相色谱分析
...radedproductsofpenicillin,6-aminopenicillanicacid(6-APA),penicillinGkalium(PGK),phenylaceticacid(PAA),andtheby-productbenzylpenicilloicacid(BPA)byHPLCwasstudied.Separationconditionswereasfollows:SpherisorbC18column,250mm×4.6mmi.d.,10μm;mobilephase:V(methanol)∶V(0.004mol/LKH2PO4buffer,pH4.5)=50...
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青霉素裂解液的高效液相色谱分析
...radedproductsofpenicillin,6-aminopenicillanicacid(6-APA),penicillinGkalium(PGK),phenylaceticacid(PAA),andtheby-productbenzylpenicilloicacid(BPA)byHPLCwasstudied.Separationconditionswereasfollows:SpherisorbC18column,250mm×4.6mmi.d.,10μm;mobilephase:V(methanol)∶V(0.004mol/LKH2PO4buffer,pH4.5)=50...
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基因芯片筛选口腔颌面鳞癌顺铂耐药和敏感相关基因
...和FANCONI,GRP58,FLJ12089和SPINT-2基因上调,而FOSL1,MRPS27和PGK-1基因下调(OncolRep.2005Apr;13(4):709-14.)。韩国蔚山大学为了确定调节顺铂敏感的相关基因,采用基因芯片技术,研究了源自顺铂敏感和顺铂抵抗的细胞株的基因表达谱,...
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RegulationofTypeIICollagenSynthesisduringOsteoarthritisbyProlyl--Hydroxylases
...alHIF-1targetgenes,glucosetransporter-1(Glut-1)andphosphoglyceratekinase-1(PGK-1).【关键词】regulationcollagensynthesisosteoarthritisprolyl--hydroxylasesMaterialsandMethodsPatientsandSamplesforImmunohistochemistryCartilage/bonesampleswereobtainedfrompatientswhowerehospitalizedintheDivisionofOrt...
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CriticalrolesoftheimmunoglobulinintronicenhancersinmaintainingthesequentialrearrangementofIgHandIgkloci
...r;MSRE-QPCR,methylation-sensitiverestrictionenzymereal-timequantitativePCR;PGK,phosphoglycerolkinase;RS,recombiningsequence;RSS,recombinationsignalsequence;SE,signalend.M.A.InlayandT.Lincontributedequallytothiswork.TheIgandTCRgenesareassembledthroughthesomaticrecombinationofV(Variable),D(Diversity),...
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ComprehensiveTranscriptomeofProteasesandProteaseInhibitorsinVascularCells
...ddedtoeachreaction.Forthispurpose,weused6humanhousekeepinggenes:PPIA,GAPDH,PGK1,EEF1a,RPL13A,andubiquitin.Moreover,usingGeNorm,7weselectedforeachtime2ofthemoststablehousekeepinggenesacrossallofthespecimensandusedtheirgeomeansfornormalization.NormalizeddataarepresentedinTables1,2,and3asrelativegeneco...
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ProtectionofHIF-1-deficientprimaryrenaltubularepithelialcellsfromhypoxia-inducedcelldeathisglucosedependent
...tcaacacc-3‘.PrimersequencesandreactionconditionsforHif1aexon-2,Bax,Bnip3,Pgk,and-actinwereusedasdescribedbefore(6).Chemicals.2-Deoxyglucose(2-DOG)andphloretinwerepurchasedfromSigma.Statisticalanalysis.Atwo-tailedStudent’st-testwasusedforstatisticalanalysis.Pvaluesof<0.05wereconsideredstatistical...
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BaffledbyBafilomycin:AnAnticancerAgentThatInducesHypoxia-InducibleFactor-1Expression
...aspectsofcancerbiology:VEGFandSDF1stimulatetumorangiogenesis;GLUT1,ENO1,andPGK1stimulateglucosetransportandaerobicglycolysis;EPO,IGF2,andTGFAparticipateinautocrinegrowthfactorsignaling;andmultidrugtransportersABCB1andABCG2promotechemotherapyresistance(HirotaandSemenza,2006).Inaddition,HIF-1activates...
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X染色体与肿瘤的关系研究进展
...21)、TFM(Xp11-q13)、AR(Xp11-q21)、XCE(Xp11.2-q21.1)、PGK(Xp13)、CID(Xp13-q22)、GLA(Xp21-q24)、Alport(Xp22)和HPRT(Xp26)。常用的限制性内切酶有HpaⅡ、HhaⅠ、BgⅠ等[3,4,7,11,12]。用这些方法研究证明急性非淋巴细胞...
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HyperproductionofHyaluronaninNeu-InducedMammaryTumorAcceleratesAngiogenesisthroughStromalCellRecruitment
...ingtoexpressionofHas2mRNA.White,gray,andblacktrianglesrepresentCAGpromoter,PGK-Neo,andHas2-R7primers,respectively.B:PCRanalysisofCre-mediatedgenomicDNArecombination.GenomicDNAsampleswereisolatedfromNeu-initiatedmammarytumors.PCRscreening(#1)withtheCAGpromoterandPGK-Neoprimersgavetheanticipated360-bp...
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15d-ProstaglandinJ2ProtectsBrainFromIschemia-ReperfusionInjury
...tion-defectiverecombinantadenoviral(rAd)vectorahumanphosphoglyceratekinase(PGK)promotertodriveCOX-1(Adv-COX-1),greenfluorescentprotein(Adv-GFP),orPGKalonetoserveasthecontrol(Adv-PGK).IntracerebralVentricularInfusionofAdenoviralConstructsand15d-PGJ2Theprocedurewasperformedaspreviouslydescribed.4Brief...
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低氧诱导因子-1α与肿瘤演进
...白GLUT1、GLUT3以及糖酵解酶HK1、HK3、PFKL、ALDA、ALDC、GAPDH、PGK1、PGM、ENO1、PKM、LDHA[12]。在人类乳腺癌细胞实验中发现,HIF-1α与糖代谢活性密切相关[13]。巨噬细胞依赖于糖酵解产生的ATP而维持其功能,HIF-1α缺失的巨噬细胞ATP...
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MutantFibrinogenClearedfromtheEndoplasmicReticulumviaEndoplasmicReticulum-AssociatedProteinDegradationandAutophagy
...lotanalysisforthepresenceofthecytosolicmarkerproteinphosphoglyceratekinase(PGK),anERluminalmarkerproteinBiP,andfibrinogen.PGKwasseenonlyinthecytosolfraction,asexpected.Conversely,HMWAguadillaDalongwiththeERluminalchaperoneBiPresidedexclusivelywithinthemicrosomefraction(Figure5A).Todeterminewhetherth...
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AbsenceofbacteriallyinducedRELM?reducesinjuryinthedextransodiumsulfatemodelofcolitis
...t.(A)Schematicdiagramoftheknockoutstrategy.Thephosphoglyceratekinase–Neo(PGK-Neo)cassettewasusedtodisrupt2323bpoftheRELM?genelocus,replacingthepromoterregionandthefirst2exons.(B)GenomicPCRusingprimersshowninA.Amplificationofa295-bpproductbyprimersAandBindicatethewild-typeallele(lowerband),whereasa...
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Leisure-timeactivityisanimportantdeterminantoflong-termweightmaintenanceafterweightlossintheSibutramineTrialonObesityReductionandMaintena
...H);theDirectorateofDiabetesMetabolism,RoyalLondonUniversityHospital,London(PGK);theObesityUnit,HuddingeUniversityHospital,Huddinge,Sweden(SR);andtheRowettResearchInstitute,Aberdeen,UnitedKingdom(WPJ).2SupportedbyKnollPharmaceuticals,Nottingham,UnitedKingdom.3AddressreprintrequeststoMAvanBaak,Departm...
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与肾脏发生相关的基因研究进展
...合在肾小球发生中的作用[15],Soriano[16]将lacZ基因与pgk-neo基因相连接以观察PDGFβ基因启动部位在杂合子变异体上的表达。PDGFβ基因在肾小球发生早期表达在输尿管芽,在发生后期主要局限于系膜细胞。 PDGFB与PDGFR基因变...
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HypoxicinductionofCtgfisdirectlymediatedbyHif-
...lability(VEGF,erythropoietin)andenhancingglucoseuptakeandmetabolism(Glut-1,PGK).Inthispaper,wehaveusedprimarytubularepithelialcellculturesfromatetracycline-inducible-Hif-1knockoutmurinemodeltofurtherelucidatetheroleofHif-1inthehypoxic-inductionofCtgfexpression.Weshowthathypoxiaresponseelementspresen...
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丝状真菌瑞氏木霉生产重组蛋白的分子生物学研究进展
...报道从瑞氏木霉中分离了非纤维素酶基因的启动子,包括pgk启动子、pkiA启动子、tefl启动子和一个在葡萄糖培养基上强表达但未知的cDNA1的启动子,这些启动子可以在葡萄糖培养基上启动合成所需要的纤维素酶,产生的酶制剂基...