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Healthyintakesofn3andn6fattyacids:estimationsconsideringworldwidediversity
...#150;3fattyacidsinfluencestissuecompositionsofn3long-chainfattyacids(LCFAs:eicosapentaenoic,docosapentaenoic,anddocosahexaenoicacids)andrisksofcardiovascularandmentalillnesses.Objective:Weaimedtoestimatehealthydietaryallowancesforn3LCFAsthatwouldmeetthenutrientrequirementsof9798%of...
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中链甘油三酯对糖尿病患者体重和体脂的影响研究
...在线粒体内被氧化。中链脂肪酸(MCFA)不像长链脂肪酸(LCFA)那样需要依赖肉毒碱的转运,可以自由穿越线粒体膜后进行β氧化,所以MCFA比LCFA具有较快的氧化清除率。有研究表明MCFA在肝内氧化代谢及转化速率和葡萄糖一样快[5...
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Restorationofhypothalamiclipidsensingnormalizesenergyandglucosehomeostasisinoverfedrats
...dationissufficienttorestorethehypothalamiclevelsoflong-chainfattyacyl-CoAs(LCFA-CoAs)andtonormalizefoodintakeandglucosehomeostasisinoverfedrats.Tothisend,wetargetedtheliverisoformofcarnitinepalmitoyltransferase-1(encodedbytheCPT1Agene)byinfusingeitherasequence-specificribozymeagainstCPT1Aoranisoform...
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Criticalroleofstearoyl-CoAdesaturase–1(SCD1)intheonsetofdiet-inducedhepaticinsulinresistance
...separameterswhencomparedwithSCrats(whitebars).(C)OFcausedadecreaseinhepaticLCFACoAlevels,whichwererestoredtoSClevelsbyScd1ASOtreatment(topleftpanel).LiverScd1deficiencyinOFanimals(blackbars)resultedina3.5-foldincreaseinliverTG(toprightpanel)whencomparedwithSCRASOOFanimals(graybars).JNKphosphorylatio...
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InsitucharacterizationofCD4+Tcellbehaviorinmucosalandsystemiclymphoidtissuesduringtheinductionoforalprimingandtolerance
...ng(2,16).Inchallengeexperiments,animalswereimmunizeds.c.with100μgOVAin50μlCFA10dafterfeedingandserumanti-OVAIgG1levelsdetermined21dlaterbyELISAasdescribedpreviously(2,16–19).Two-photonexcitationmicroscopyExcisedorinsitumesentericandPLNswereimagedessentiallyasdescribedpreviously(9–11).Theexcise...
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LeptinneutralizationinterfereswithpathogenicTcellautoreactivityinautoimmuneencephalomyelitis
...ractiveinductionofEAE,mice(n=6pergroup)wereimmunizeds.c.intheflankwith100μlCFA(Difco;BDDiagnostics—DiagnosticSystems)emulsifiedwith100μgPLP139–151peptideonday0andwith200ngpertussistoxin(Sigma-Aldrichs.r.l.)i.p.ondays0and1.Controlmice(n=5pergroup)wereinjectedwithCFAemulsifiedwithPBSpluspertussi...
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Identificationandcharacterizationofnoncalcemic,tissue-selective,nonsecosteroidalvitaminDreceptormodulators
...5(MEVGWYRSPFSRVVHLYRNGK,PeptidesInternationalInc.)emulsifiedinatotalof200μlCFA(BDDiagnostics)containing500μgM.tuberculosisH37Ra(BDDiagnostics).Immunizedmiceweretreatedwithvehicle(sesameoil),LY2108491(10and100μg/kg),orLY2109866(10and100μg/kg).Tendayslater,splenocyteswereisolatedbyhomogenizingsple...