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南海海洋所揭示L-别异亮氨酸的生物合成机制
...Soc.(2016,138(1),408-415)发表文章首次阐述天然产物中L-allo-Ile结构单元的生物合成机制,论文被同期JACSSpotlights(2016,138,461)作为研究亮点评述。该成果为研究其它生物体中L-allo-Ile的来源和生物医疗提供了思路;“氨基转移酶”和异...
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白细胞介素4受体α链基因多态性与支气管哮喘的相关性研究
...440-44310(广州)为了检测白细胞介素4受体α链(IL-4Rα)基因Ile50Val位点的多态性在汉族人群中的分布频率,探讨Ile50Val位点多态性与支气管哮喘(简称哮喘)的相关性。研究者对2002至2004年间暨南大学附属第一医院呼吸科门诊及住院患...
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SingleMutationsatAsn295andLeu305intheCytoplasmicHalfofTransmembrane-HelixDomain7oftheAT1ReceptorInducePromiscuousAgonistSpecificityfor
...cells—apseudo-constitutiveactivity.ItissurprisingthattheAngIIanalog[Sar1,Ile4,Ile8]AngIIandthenativeangiotensinIIfragmentsAng1-7,AngIV,andAng5-8,whichareinactiveinactivatingthewild-typereceptor,activatedN295SandL305Q.ResultsgeneratedbyloweringtheNa+concentrationsuggestthatthemutantN295SandL305Qmay...
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系统性红斑狼疮患者白细胞介素-4受体基因多态性的研究
...)为了初步探讨白细胞介素-4受体(IL-4R)的两个氨基酸位点ile50val和gln576arg多态性与系统性红斑狼疮(SLE)患者发病的相关性。研究者采用聚合酶链反应-单链构象多态性(PCR-SSCP)和聚合酶链反应限制性片段多态性(PCR-RFLP)及DNA测序方法...
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磷脂酰肌醇3-激酶P85α亚单位基因Met326Ile变异与2型糖尿病的相关关系
...为了探讨磷脂酰肌醇3-激酶(PI3-K)基因P85α调节亚单位Met326Ile突变及基因表达与2型糖尿病(T2DM)的关系。研究者采用双引物竞争PCR法检测T2DM者240例及正常对照(NC)组150例Met326Ile突变,对部分受检者的外周血白细胞RNA进行RT-PCR扩增,观...
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STK15基因单核苷酸多态与大肠癌风险
...中华肿瘤杂志2006Vol.28No.1P.43-467(北京)为了探讨STK15Phe31Ile基因单核苷酸多态与大肠癌风险的关系。研究者以聚合酶链反应(PCR)和限制性片段长度多态性(RFLP)分析方法,检测了283例大肠癌患者和283例配对的正常对照者STK15Phe31Ile基...
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Thehumanorganicaniontransporter3(OAT3;SLC22A8):geneticvariationandfunctionalgenomics
...rogeneityamongOAT3variants,withthreevariants(p.Arg149Ser,p.Gln239Stop,andp.Ile260Arg)thatresultedincompletelossoffunction,andseveralotherswithsignificantlyreducedfunction.Oneofthemorecommonvariants(p.Ile305Phe),foundin3.5%ofAsian-Americans,appearedtohavealteredsubstratespecificity.Thisvariantexhibit...
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ActivationoftheAngiotensinIIType1ReceptorLeadstoMovementoftheSixthTransmembraneDomain:AnalysisbytheSubstitutedCysteineAccessibilityMetho
...monium(MTSEA).Thistreatmentledtoasignificantreductioninbindingof125I-[Sar1,Ile8]AngIItotheF249C,H256C,T260C,andV264Cmutantreceptors,suggestingthattheseresiduesorientthemselveswithinthewater-accessiblebindingpocketoftheAT1receptor.ItisnoteworthythatthispatternofacquiredMTSEAsensitivitywasalteredforTM...
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利用噬菌体肽库确定IL-2Rα表位序列①
...守序列:Ser-Ser-Phe包含在4个序列为Ser-Ser-Phe-Tyr-Pro(Leu)-Ile的噬菌体克隆中;Ser-Ser-Arg包含在10个序列为Lys-Phe(Cys)-Ser-Ser-Arg-Leu(Lys)的噬菌体克隆中(见表2)。表2 DNA测序所得的氨基酸序列 Tab.2 AminoacidsequencesdeducedfromDNAsequencesNo...
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晚期结直肠癌GSTP1基因多态性与奥沙利铂化疗效果关系
【摘要】目的探讨谷胱甘肽S转移酶P1(GSTP1)基因Ile105Val(A→G)多态性与晚期结直肠癌对以奥沙利铂为主方案化疗效果的关系。方法Ⅳ期结直肠癌病人102例,化疗前取静脉血应用TaqMan-MGB探针等位基因分型技术检测GSTP1基因Ile105Val的多...
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内皮脂肪酶与血脂和冠心病的关系
...有潜在功能,其中有4个可引起氨基酸替换(Gly26Ser,Thr111Ile,Thr298Ser,Asn396Ser),其他2个变异在EL基因启动子区域(-410C/G,-303A/C);Mank-Seymour等[18]在2004报道了13个新的EL基因单核苷酸变异,12个与HDL-C水平有显著相关性,其中5个单核苷...
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Renalmedullarytissueoxygenationisdependentonbothcorticalandmedullarybloodflow
...itherthecortexormedulla.Inanesthetizedrabbits,renalarterialinfusionof[Phe2,Ile3,Orn8]-vasopressinselectivelyreducedmedullaryperfusionandPO2(54±24and50±10%,respectively)butdidnotsignificantlyaffectcorticalperfusionortissueoxygenation.Incontrast,stimulationoftherenalnervesresultedinrenalcorticalisch...
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药物代谢酶CYP1家族相关基因的研究进展
...限制性内切酶MspI酶切后,可明确MspI多态性类型。1.1.2m2是Ile/Val多态性(CYP1A1*2C),又称为Exon7多态性。它也有三种基因型:野生型(Ile/Ile型),杂合型(Ile/Val型)及突变纯合型(Val/Val型)三种形式。m2与m1位点突变高度关联。1.1.3m3...
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单核苷酸多态性与静脉血栓栓塞症的相关性分析
...,静脉血栓栓塞症与Asp79His、Arg306The、Arg306Gly、Arg506Gln和Ile359The多态性基本无关;His1299Arg在VTE组中的分布频率高于对照组,但差异无统计学意义。Hisl299Arg与Metl736Ⅴal和As02194Gly多态性在人群中有连锁不平衡特性。深静脉血栓和肺...
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一种现存的氨基酰-tRNA合成酶(AaLeuRS)具有祖先编校特性
...先编校特性的氨基酰-tRNA合成酶,揭示了AaLeuRS可能有着比IleRS及ValRS更为原始的编校特性,其编校结构域可能保留了三种酶共同的祖先编校结构域,该发现为研究氨基酰-tRNA合成酶催化特异性进化提供了的重要线索。氨基酰-tRNA合...
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3-2-5氨基酸的活化与转运
...酸的活化过程。到目前为止,另发现有二种氨基酸(Gln和Ile)的活化有其特殊条件。Gln-tRNA复合物的形成如下,即先由一个Glu经GluRS作用接在tRNAGln上,形成Glu-tRNA;然后再经转氨基酶作用,将Glu(或Asn)的酰胺基接到Glu-tRNA复合物...
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一例乙型肝炎患者体内不同HBV克隆的序列差异
...并提出第三对等位基因亚型决定簇(i/t)的概念,即aa126位Ile为i亚型,aa126位Thr为t亚型,人们才开始认识到这一位点在血清学分型中的重要性[10]。由于迄今已发表的所有adr亚型HBV在该位均为Ile密码子ATT,No.24患者感染的HBV显然是一...
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ProteochemometricMappingoftheInteractionofOrganicCompoundswithMelanocortinReceptorSubtypes
...receptorsubtypesisadifficulttaskthatwouldbecomesimplifiedifonehadinhanddetailedknowledgeofthedeterminantsforligand-receptorinteraction.Wehaverecentlyinventedtheproteochemometricmodelingtechnologyfortheanalysisofprotein-ligandinteractions(Wikbergetal.,2003,2004).Thistechnologyisparticularlysuitedtoga...
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GSTP1在东北地区酒精性肝病发病机制中的研究
...[2]。是GSTs超基因家族中π家族的成员。GSTP1*D等位基因(Ile105-Val114)已被证实:具有Val105的酶比具有Ile105的酶对多环芳烃二醇环氧化作用的效力高7倍,GSTP1第5外显子的一个位点的A→G突变导致两个氨基酸的酶的活性部位被取代即...
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NeutralaminoacidtransportmediatedbyorthologofiminoacidtransporterSIT1/SLC6A20inopossumkidneycells
...transportresembles,butdoesnotmatch,thatdescribedformammalianB0AT1.UsingRNAsilencingtechniquesinOKcellsandX.laevisoocyteexpression,weshowherethattheB0-liketransportofOKcellsismediated,atleastinpart,bytheorthologofthemammalianIMINOsystemtransporterSIT1(SLC6A20).EXPERIMENTALPROCEDURESCellculture.Theest...
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Mass-SpectrometricIdentificationofaNovelAngiotensinPeptideinHumanPlasma
...LDI-TOF/TOF)revealedanangiotensinoctapeptidewiththesequenceAla-Arg-Val-Tyr-Ile-His-Pro-Phe,whichdiffersfromAngIIinAla1insteadofAsp1.Des[Asp1]-[Ala1]-AngII,inthefollowingnamedAngiotensinA(AngA),ismostlikelygeneratedenzymatically.Inthepresenceofmononuclearleukocytes,AngIIisconvertedtoAngAbydecarboxyla...
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邻苯二甲醛柱前衍生反相高效液相色谱法测定水牛角中氨基酸的含量
...mnpackedwith5μmODS(octadecylsilance).AnacetatebufferatpH5.6wasusedasthemobilephaseA,whilemethanolwasemployedasthemobilephaseB.Lineargradientelutionwasadoptedwithafluorescentdetector(Ex=340 nm,Em=460 nm).Twelveaminoacidsinbuffalohornwereseparatedanddetectedin32min.Fromtheresults,agoodlinearrelat...
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关于食道癌相关基因的研究报告
...间的交互作用。在有抽烟嗜好的病人中,GSTM1null型和GSTP1ile/ile基因型有更高罹患食道癌风险。而饮酒且同时带有GSTM1null和GSTP1val/val基因型,会造成中度的食道癌风险。GST基因型于职业上的暴露之间的交互作用,并不会影响食道癌...
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人可溶性IL-6R及其突变体基因在COS7细胞中的表达及活性分析①
...术扩增天然人sIL-6R基因片段,然后将第280位His残基突变成Ile。天然基因和突变体基因分别转染COS7细胞,经ELISA和Westernblot检测证实转染细胞上清中的表达产物,并利用BindingassayELISA和生物学功能分析法检测表达产物与IL-6的结合能...
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IschemicNeoangiogenesisEnhancedby2-AdrenergicReceptorOverexpression
...doIaccarino,MicheleCiccarelli,DanielaSorriento,GennaroGalasso,AlfonsoCampanile,GaetanoSantulli,ErsiliaCipolletta,VincenzoCerullo,VincenzoCimini,GiovannaGiuseppinaAltobelli,FedericoPiscione,OrnellaPriante,LucioPastore,MassimoChiariello,FrancescoSalvatore,WalterJ.Koch,BrunoTrimarcotheDipartimentodiMed...
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BindingSiteofaNovelKv1.5Blocker:A“FootintheDoor“againstAtrialFibrillation
...tagenesisoftheporedomainofKv1.5identifiedtheaminoacidsThr479,Thr480,Val505,Ile508,Val512,andVal516asimportantresiduesforblockbyAVE0118.AhomologymodeloftheporeregionofKv1.5predictsthatthesesixresiduesfacetowardthecentralcavityofthechannel.Inaddition,mutationoftwootherS6residues(Ile502andLeu510)thatar...
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肽的溶解性及储存
...极性氨基酸(疏水性)小的:Cys、Pro、Ala、Thr大的:Val、Ile、Leu、Met、Phe中性氨基酸大的:Trp、Tyr、His极性氨基酸(亲水性)小的:Ser、Gly、Asp、Asn大的:Glu、Gln、Lys、Arg可溶性的问题许多合成的肽粗品,尤其是那些经过几次沉...
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疟原虫抗药性的遗传学研究进展
...报道了6个DHFR基因编码区变异:108位Ser→Asn或Thr,51位Asn→Ile,59位Cys→Arg,16位Ala→Val,164位Ile→Leu。单一点突变所致DHFR108位Ser变异成Asn即可产生乙胺嘧啶抗性,但对环氯胍的反应性仅稍有降低。对乙胺嘧啶产生高度抗性则需其他位...
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Thebeneficialeffectof-linolenicacidincoronaryarterydiseaseisnotquestionable
...talityfromCAD(P0.04)andfromallcauses(P0.02).TheintakeofALAinthehighestquintilewas3.2-foldthatinthelowestquintile.Morerecently,2largeprospectivestudiesin76 283nurses(4)and43 757healthprofessionals(5)showedthatALAwastheonlyfattyacidthatprotectedagainstcardiacdeath(4)andagainstnonfatalmyocard...
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急性呼吸窘迫综合征相关基因的研究
...R4(Asp299Gly)多态性进行分析,发现人TLR4基因的Asp299Gly和Thr399Ile多态性与吸入LPS后低应答相关,增加了由革兰阴性菌引起的脓毒血症的易感性。在这些人中,上皮细胞对LPS的刺激免疫应答能力减弱。进一步研究发现Asp299Gly比Thr399Ile更能...