-
Scenesse获批准成为治疗遗传性疾病的新方法
...e,与红细胞生成protoporPHyria防止光毒性首先在成人医学(EPP)。 在暴露于阳光之后,EPP患者会感觉到暴露于阳光的皮肤有一种刺痛感,延长暴露可导致一种令人伤残的疼痛,随后出现红肿。Scenesse计数器疾病的影响,从而影...
-
抗紫外线疫苗即将面世可助日光过敏者防晒
...疫苗”之称的它,除了能防癌外,还能有效用于那些患有EPP(对自然光过敏)的人。自然光的正常照射,往往使他们的皮肤起泡、灼伤,“疼痛难忍”。 通过将谷粒大小的植物插入患者腰部,并复制人体对紫外线及有害辐射的...
-
“紫外线疫苗”即将面世可帮助日光过敏者“防晒”
...疫苗”之称的它,除了能防癌外,还能有效用于那些患有EPP(对自然光过敏)的人。自然光的正常照射,往往使他们的皮肤起泡、灼伤,“疼痛难忍”。通过将谷粒大小的植物插入患者腰部,并复制人体对紫外线及有害辐射的天然...
-
GC-2050气相色谱仪
...处理系统,并配有通用打印机端口(HOST、USB、SPP、ECP、EPP),使GC9160气相色谱依具有 单机可完成图谱显示、数据分析、结果打印的独特功能。 自我诊断功能,能显示故障部位。 触摸式键盘,方便使用者对各项操作...
-
马来西亚推动使用生物润滑油
...从2011年开始将资本支出激励性补贴(根据入口点计划6,即EPP6)发放给那些有意进一步从事棕榈油下游业务的公司。这些补贴用于生产附加值更高的棕榈油衍生物,比如表面活性剂、农业化学品、生物多元醇、生物润滑油、甘油衍...
-
不稳定骨盆骨折的损伤控制
...1]。2.8骨盆填塞腹膜外填塞技术(extraperitonealpelvicpacking,EPP)成功应用于膀胱和骶骨周围间隙的止血。采用骨盆C型钳或外固定支架提供骨盆后环的即时稳定性,提供了骨盆填塞的机械稳定性和骨折复位而减少骨折出血。骶骨周围...
-
MonitoringofCytomegalovirusInfectioninSolid-OrganTransplantRecipientsbyanUltrasensitivePlasmaPCRAssay
...enefitofanultrasensitiveplasma-basedPCRassayforrenaltransplantrecipients.Thepp65CMVantigen(pp65Ag)assayusingleukocyteswasemployedasaroutinetestforthemonitoringofCMVin23transplantrecipients.Wecomparedthepp65antigenemiawiththeCMVloadquantifiedbyanultrasensitivePCR(US-PCR)withalimitofdetectionof20CMVDN...
-
Heme-regulatedeIF2kinasemodifiesthephenotypicseverityofmurinemodelsoferythropoieticprotoporphyriaand?-thalassemia
...eofHRIin2mousemodelsofhumanrbcdisorders,namelyerythropoieticprotoporphyria(EPP)and?-thalassemia.Inbothcases,lackofHRIadverselymodifiesthephenotype:HRIdeficiencyexacerbatesEPPandrenders?-thalassemiaembryonicallylethal.ThisstudyestablishestheprotectivefunctionofHRIininheritedrbcdiseasesinmiceandsugges...
-
Understandingglobinregulationin?-thalassemia:it’sassimpleas,?,,
...RIdeficiencyisalsoshowntoincreasetheseverityoferythropoieticprotoporphyria(EPP),adiseasecharacterizedbydefectivehemesynthesis.NormalHRIactivityisshowninEPPtopreventthemoresevereaccumulationofbothfree-andfree?-globinchains,whichformerythroidcellinclusionsandaretoxictocells.ThisisdocumentedinHRI–/...
-
InfluenceoftriacylglycerolstructureonthepostprandialresponseoffactorVIItostearicacidrichfats
...iacylglycerolwascollectedbyfinger-prick1,2,4,and5hpostprandiallyintoamicro-Eppindorftubecontaining1mgdipotassiumEDTA.Thebloodsampleswereprocessedwithin1hofcollection.AnalyticmethodsPlasmatriacylglycerolandtotalandHDL-cholesterolconcentrationsweremeasuredbyenzymaticassayaspreviouslydescribed(2).Plasm...
-
SensitiveEDTA-BasedMicrobiologicalAssaysforDetectionofMetallo--LactamasesinNonfermentativeGram-NegativeBacteria
...microbiology.Clin.Microbiol.Rev.3:219-226.Khler,T.,M.Michea-Hamzehpour,S.F.Epp,andJ.C.Pechere.1999.CarbapenemactivitiesagainstPseudomonasaeruginosa:respectivecontributionsofOprDandeffluxsystems.Antimicrob.AgentsChemother.43:424-427.Lee,K.,Y.Chong,H.B.Shin,Y.A.Kim,D.Yong,andJ.H.Yum.2001.ModifiedHodge...
-
SrcFamilyKinaseInhibitorsBlockAmphiregulin-MediatedAutocrineErbBSignalinginNormalHumanKeratinocytes
...iveininhibitingwoundclosure,withmarkedinhibitionbyaslittleas0.2ePD173953or5ePP1.Aspreviouslydemonstrated(Stolletal.,2003),U0126alsoinhibitedwoundclosure,demonstratingeffectivenessatconcentrationsaslowas2e.ItwassurprisingthatlowdosesofbothSFKIsproducedalong-lastinginhibitionofNHKproliferationandmigra...
-
ScavengerReceptorClassBTypeIMediatestheSelectiveUptakeofHigh-DensityLipoprotein–AssociatedCholesterylEsterbytheLiverinMice
FromUniversityHospitalHamburg-Eppendorf(M.B.,A.E.,J.H.,B.B.,H.G.,M.M.,F.R.),Hamburg,Germany;andtheCollegeofPhysiciansandSurgeonsofColumbiaUniversity(R.R.),NewYork,NY.CorrespondencetoDrFranzRinninger,UniversityHospitalHamburg-Eppendorf,DepartmentofMedicine,Martinistrasse52,20246Hamburg,Germany.E-mail...
-
MRImagingoftheMetacarpophalangealJointsoftheFingersPartI.ConventionalMRImagingandMRArthrographicFindingsinCadavers1
...chmentsandbodiesofthepalmarplates(PPs),therecessesofthedistalattachmentofthePP,andthedeeptransversemetacarpalligament(DTML).3.Theextensorhoodformedbythefollowing:radialandulnarsagittalbands,transversefibersoftheinterosseoustendinouspartoftheextensorhoodontheradialandulnarsides(Fig2),andfibrousconnec...
-
CellCycleSignalingbyEndothelin-1RequiresSrcNonreceptorProteinTyrosineKinase
...wellplates.Cells,serumstarvedfor24hinDMEM/0.5%FBSfor24,werepretreatedwith10ePP2orPP3for1hbeforeaddingeither100nMendothelin-1or20%FBS.CellswerefixedandstainedforBrdUincorporationasdescribedabove.WesternBlotting.WesternblottingforPTyrSrc(416)andcyclinD1inHMCswasperformedasreportedpreviouslybyuswithmin...
-
NuclearLocalizationofNADPH:Cytochromec(P450)ReductaseEnhancestheCytotoxicityofMitomycinCtoChineseHamsterOvaryCells
...ygenradicalsandotheroxygen-derivedspecies,inFreeRadicalsinBiologyandMedicinepp23eC85,ClarendonPress,Oxford,UK.HodnickWFandSartorelliAC(1993)ReductiveactivationofmitomycinCbyNADH:cytochromeb5reductase.CancerRes53:4907eC4912.HoeyBM,ButlerJ,andSwallowAJ(1988)ReductiveactivationofmitomycinC.Biochemistry...
-
ExpressionandPhosphorylationoftheNa-PumpRegulatorySubunitPhospholemmaninHeartFailure
.../L):50Tris,1dithiothreitol(DTT),1MnCl2,and2Uproteinphosphate1a(PP1a)plus0.1ePP2a.ImmunoblotsHomogenatesandcelllysates(30eprotein/lane)weresize-fractionatedonSDS-PAGE(10%forNa/K-ATPase;15%forPLM,12%forimmunoprecipitations).Proteinsweretransferredto0.20-enitrocellulose(NaVO3wasaddedtothetransferbuffer...
-
DetectionofPseudomonasaeruginosaProducingMetallo--LactamasesinaLargeCentralizedLaboratory
...monasaeruginosa.Clin.Infect.Dis.37:26-32.Kohler,T.,M.Michea-Hamzehpour,S.F.Epp,andJ.C.Pechere.1999.CarbapenemactivitiesagainstPseudomonasaeruginosa:respectivecontributionsofOprDandeffluxsystems.Antimicrob.AgentsChemother.43:424-427.Lagatolla,C.,E.A.Tonin,C.Monti-Bragadin,L.Dolzani,F.Gombac,C.Bearzi,...
-
SrcFamilyKinaseActivityIsRequiredforMurineEmbryonicStemCellGrowthandDifferentiation
...pendentofthepresenceofLIF.ThenextSFKinhibitortestedwasthepyrazolo-pyrimidinePP2,anotherbroad-spectruminhibitorofSFKactivity(Hankeetal.,1996).AsshowninFig.4A,strongSFKinhibitionwasobservedinEScellsaftertreatmentwith5μMPP2,withnearlycompleteinhibitionat10μM.AsobservedwithSKI-1,partialinhibitionofSFK...
-
第四节 石蜡包埋组织的DNA提取及其应用
...的paget细胞是来源于腺癌细胞而不是表皮的角质层细胞。EPP中的paget细胞也来源于腺癌细胞,但这种腺癌细胞与MPD中的腺癌细胞不同,因为两者基因表达不同。 3.遗传病的基因诊断遗传病的回顾性家族调查,可通过石蜡包埋...
-
DomainsNecessaryforG12BindingandStimulationofProteinPhosphatase-2A(PP2A):IsG12aNovelRegulatorySubunitofPP2A?
...orminganA-Ccoreenzyme.BsubunitsreversiblyinteractwiththecoreenzymetoregulatePP2Afunction,andotherproteinscanmimic(replace)Bsubunits.WedemonstratedpreviouslyG12stimulationofPP2AphosphataseactivityinvitroandinCOScells(Zhuetal.,2004).ManysignalingpathwaysshareinvolvementofG12andPP2A,althoughuntilrecent...
-
第二节 脂质代谢
...物(IPP及DPP)缩合成15C的焦磷酸法呢酯(farnesylpyrophosfhate,EPP)。二分子15C焦磷酸法呢酯在内质网鲨烯合酶(squalenesynthase)的作用下,再缩合,还原即生成30C的多烯烃-鲨烯(squalene)。图1-3胆固醇合成代谢图 (3)胆固醇的合成。鲨烯...
-
Methylmercury-InducedIncreaseofIntracellularCa2+IncreasesSpontaneousSynapticCurrentFrequencyinRatCerebellarSlices
...mentcausedalargeandtransientincreasebeforeeventualsuppressionoffrequencyofMEPPsatneuromuscularpreparations(Juang,1976;AtchisonandNarahashi,1982).TimestoonsetofincreasesinMEPPfrequencywereapproximately30to40minand5to15min,respectively,afterMeHgexposureto20and100µMMeHg(AtchisonandNarahashi,1982)...
-
MolecularApproximationsbetweenResidues21and23ofSecretinandItsReceptor:DevelopmentofaModelforPeptideDockingwiththeAminoTerminusoftheSe
...es37to122.Themodelswerethencompletedbyattachingmissinghydrogens,assigningECEPP/3atomtypesandcharges(Nemethyetal.,1992),followedbyregularizationtoimposeidealcovalentgeometriesonallbondanglesandbondlengths.EighthundredandthreeNMRrestraintswereconvertedtoICM-readableformatandwereimposedonthemodelsusing...
-
Myastheniagravis:past,present,andfuture
...eAChRionchannelopening,permittinginfluxofNa+intothemusclefiber.TheresultingEPPactivatesvoltage-gatedNa+channelsatthebottomofthefolds,leadingtofurtherNa+influxandspreadingoftheactionpotentialalongthemusclefiber.Otherproteins,includingRapsyn,MuSK,andagrin,whichareinvolvedinAChRclustering,arealsopresen...
-
MCP-1contributestomacrophageinfiltrationintoadiposetissue,insulinresistance,andhepaticsteatosisinobesity
...oxyl-terminaltails.Proc.Natl.Acad.Sci.U.S.A.91::2752-2756.Schweickart,V.L.,Epp,A.,Raport,C.J.,andGray,P.W.2000..CCR11isafunctionalreceptorforthemonocytechemoattractantproteinfamilyofchemokines.J.Biol.Chem.275::9550-9556.Lu,D.etal.2005..CloningandfunctionalcharacterizationoftherabbitC-Cchemokinerecep...
-
Proteinphosphatase2AisanegativeregulatorofIL-2productioninpatientswithsystemiclupuserythematosus
...chasAlzheimerdisease(41)andOpitzBBB/Gsyndrome(42).WepresentdatathatintroducePP2AasanovelregulatoroftheIL2promoteractivityandIL-2production.WereportherethattheproteinandcatalyticactivityofPP2AisincreasedinpatientswithSLEbutnotinpatientswithRAandthatitisresponsible,atleastinpart,forthedecreasedproduct...