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IschemicNeuroprotectionWithSelective-OpioidReceptorAgonistIsGenderSpecific
...demonstratedpreviouslythattreatmentwithselective-opioidreceptor(KOR)agonistBRL52537hydrochloride[(±)-1-(3,4-dichlorophenyl)acetyl-2-(1-pyrrolidinyl)methylpiperidine](1)hasalongtherapeuticwindowforprovidingischemicneuroprotection,and(2)attenuatesischemia-evokedNOproductioninvivoinrats.Neuronallyderi...
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动物实验显示卡维地洛可阻断心肌β3受体
...拮抗作用研究。结果显示,在给予正常大鼠β3受体激动剂BRL37344(0.3mg/kg)后,大鼠左室压力-容积曲线右移,倾斜度减少15%。对于体外培养的大鼠心肌细胞,BRL37344(10-8M)可通过减少细胞缩短的百分数、缩短的速度和ICa,L来降低细胞收缩的...
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附录三 全血细胞培养染色技术常用试剂配制
...用现配,置冰上待用。 7.缺口平移试剂盒(Amersham和BRL公司) 8.生物素7-dATP(BRL) 9.糖原(BRL) 10.RNA酶A(Sigma) (1)储备液 称取RNA酶A1g溶于100ml醋酸钠中,置沸水中煮10min,分装后储于-20℃。 (2)...
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以Bacmid-杆状病毒-昆虫细胞系统表达人FGF-9①
... pYEX4T-1购自CLONTECHLab-oratories,INC,pFastBac和DH10Bac购自GIBCO/BRL,pCRTMⅡ质粒购自Invitrogen,菌种DH5α、INVaF由本 室保存。 1.2 酶和试剂 Trizol试剂购自GIBCO/BRL,引物经由上海生工合成,TaqDNA聚合酶、纯化质粒DNA所用的试剂盒(Wizard)...
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第四节 拟β肾上腺素能药物
第四节 拟β肾上腺素能药物 BRL26830A是一种新的β肾上腺素能促效剂,可增加产热,使代谢率上升,体脂含量减少。该药作用于特殊脂肪组织区域,不影响瘦体质密度,可减少氮丢失;对心率血压无影响。常用量每天200~400...
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格拉司琼
药物名称格拉司琼药物别名格雷西龙,康泉,Kytril,BRL-43694英文名称Granisetron说 明注射液:每支3mg(3ml)。功用作用治疗化疗或放疗所致的恶心和呕吐。用法用量将本品以注射用生理盐水20~50ml稀释后,于化疗或放疗前每日1...
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新生物燃料实验室:从“一包”变为“一桶”
...乃尔大学花费600万美元建立了一间生物燃料研究实验室(BRL)。实验室面积11000平方英尺,用于检测可持续节约型生物燃料产品。“在这里,我们可以进行生物燃料生产的所有关键步骤,”康乃尔大学赠日东北英才研究所(Northea...
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Membraneperoxidationbylipopolysaccharideandiron-ascorbateadverselyaffectsCaco-2cellfunction:beneficialroleofbutyricacid
...anTypeCultureCollection,Rockville,MD)weregrownat37 °CinMEM(Gibco-BRL,GrandIsland,NY)containing1%(byvol)penicillin:streptomycinand1%(byvol)MEMnonessentialaminoacids(Gibco-BRL)andsupplementedwith10%(byvol)decomplementedfetalbovineserum(Flow,McLean,VA).Cells(passages3040)weremaintainedw...
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CardiacAnkyrinRepeatProtein(CARP)ExpressioninHumanandMurineAtheroscleroticLesions
...tamine,and20%(v/v)humanserumsupplementedwithpenicillinandstreptomycin(GIBCOBRL).CulturedSMCswerecharacterizedbyimmunofluorescencewiththeanti-SM-actin1A4antibody.15TheSMCsshowuniformfibrillarstaining.ConfluentSMCculturesweremadequiescentbyincubationovernightinserum-freemedium,supplementedwithinsulin(...
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阳离子脂质体对人角膜基质细胞影响的实验研究
...机(Hereus公司)。主要试剂:脂质体LipofectamineTM2000(Gibco/BRL公司),DMEM培养基(Gibco/BRL公司)。1.2方法1.2.1人眼角膜基质细胞的原代培养及传代培养采用组织块培养法,将组织碎块接种于培养瓶壁上,先置入37℃、5%CO2温箱中4h,...
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MethodsfortheDetectionofD-Amino-AcidOxidase
...n.ThefirststrandofcDNAwassynthesizedusingSuperscriptPreamplificationSystem(BRL,Gaithersburg,MD).Asenseprimer(F:5‘-GGTTAACTGAGAGGGGAGTGAA-3‘)andanantisenseprimer(R:5‘-CCATAGTTGTGGATGACCTCTG-3‘)weredesignedfromthesequenceconservedincDNAsencodinghuman,mouse,rabbit,andpigD-amino-acidoxidases.The...
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β3肾上腺素能受体的心血管调节与信号转导
...;用苯肾上腺素预处理大鼠胸主动脉形成动脉环后,使用BRL37344能够引起剂量依赖性血管舒张,用SR58611A亦能得到相似的结果,且不受康加多尔预处理的影响,但β3-AR拮抗剂SR59230A却能够拮抗该效应[7],从而证实了β3-AR的...
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2AAdrenergicReceptorActivationInhibitsEpileptiformActivityintheRatHippocampalCA3Region
...formactivity.ApparentKbvaluescalculatedforatipamezole(1.7nM),MK-912(4.8nM),BRL-44408(15nM),yohimbine(63nM),ARC-239(540nM),prazosin(4900nM),andterazosin(5000nM)correlatedbestwithaffinitiespreviouslydeterminedforthe2AARsubtype(r=0.99,slope=1.0).Theseresultssuggestthat,underconditionsofimpairedGABAergi...
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Stressresponseinhibitsthenephrotoxicityofcisplatin
...s,VA).LLC-PK1cellsweregrowninDMEMsupplementedwith4mML-glutamine(DMEM,GIBCO/BRL,GrandIsland,NY),5%FBS(HycloneLaboratories,Logan,UT),penicillin(50U/ml),andstreptomycin(50μg/ml,GIBCO/BRL).Cellsweremaintainedin5%CO2-95%airat37°C.Subconfluentcultureswerepassagedevery3to4days.Fortoxicityexperiments,LLC-...
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类风湿性关节炎病人关节滑膜液与外周血中G1特异性T细胞的特点
...平均年龄52岁。 1.2 重组人G1(rG1) 人的G1用GIBCO/BRL于Bacmic线病毒表达系统产生。简述之:PCR扩增包括凝集素信号肽与第6外显子终止部分(cDNA:aggrecan cDNA由RONGHLEY博士惠赠)。在分子末端添加6个组氨酸的Tag;PCR产物连...
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Unmutatedandmutatedchroniclymphocyticleukemiasderivefromself-reactiveBcellprecursorsdespiteexpressingdifferentantibodyreactivity
...TotalRNAwasextractedfrom104–105enrichedCLLBcellsusingTRIzolReagent(Gibco-BRL,InvitrogenCorp.).RNAwasreverse-transcribedin10μl1xreactionbuffer(150ngrandomhexamerprimerN6,10mMdNTP,0.1MDTT,0.5%NP40,4URNAsin,and6UPrimeRNAseInhibitor)withSuperscriptII(Gibco-BRL,InvitrogenCorp.),andRT-PCRreactionswerep...
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Urinarykidneyinjurymolecule-1:asensitivequantitativebiomarkerforearlydetectionofkidneytubularinjury
...R,aspreviouslydescribed(4,10).Briefly,primersequenceswerecustommadebyGIBCO-BRL(Gaithersburg,MD).Kim-1primersweredesignedfromtheratKim-1sequence.Thesenseprimerwas5‘-CGGTGCCTGTGAGTAAATAGAT-3‘andantisense3‘-CTGGCCATGACACAAATAAGAC-5‘,whichamplifiedafragmentof418bp,bases1to418.Toevaluateorreducen...
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β3肾上腺素能受体基因Trp64ArgSNP与逼尿肌不稳定的研究进展
...一些与β1-AR、β2-AR呈低亲和力的β-AR激动剂,如BRL37344A[9]、CL316243和SR58611呈高效价;(3)对某些具有内在拟交感活性的β1-AR和β2-AR拮抗剂,如吲哚洛尔和烯苯氧洛尔等,则表现为部分激动剂作用[9];(4)与β1-AR、β2-...
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TissuePlasminogenActivatorInducesPancreaticCancerCellProliferationbyaNon-CatalyticMechanismThatRequiresExtracellularSignal-RegulatedKinase/A
...-1,andBxPC3cellswereculturedinDulbecco??smodifiedEagle??smedium(DMEM;GIBCO-BRL,Gaithersburg,MD)supplementedwith10%fetalbovineserum(FBS)(GIBCO-BRL),nonessentialaminoacids,penicillin,andstreptomycin.Immortalizedhumanpancreaticductalepithelial(HPDE)cellswereobtainedfromDr.M.Tsao(OntarioCancerInstitute,...
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重组人截短型胰岛素样生长因子1在大肠杆菌中的高效表达①
...的λ阻遏蛋白),由Raidoud博士惠赠;DH5αF‘IQTM购自GIBCOL/BRL公司。 1.1.2 质粒 pGEM5zf和pGEM3zf(+)质粒购自Promega公司;pBR322-IGF1质粒由北京医科大学心肺内分泌研究中心汤健教授惠赠;融合蛋白表达载体pMTY4为本室构建,它含...
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我国登革2型病毒prM基因与甲病毒载体重组RNA的构建
...。 甲病毒载体和宿主细胞株:甲病毒载体pSfV为GIBCO/BRL产品。BHK-21/13细胞株由本所细胞库提供,该细胞于含5%小牛血清,2mmol/L谷氨酰胺,10mmol/LHepes,10%tryptosephosphatebroth和1%青、链霉素的DMEM培养基中,置37℃培养至单层。 ...
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马来酸罗格列酮降低2型糖尿病外源胰岛素抵抗的临床研究
...icationofhigh-affinˉitybindingsitesfortheinsulinsensitizerrosiglitazone(BRL-49653)inrodentandhumanadipocytesusingaradioiodinatedligandforperoxisoˉmalprolifetor-activatedreceptorgamma.JpharmacolExpTher,1998,284(2):751.3SmithU.Pioglitazone:mechanismofaction.IntJClinPractSuppl,2001,9:...
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间充质干细胞体内成骨的实验研究
...力。1材料与方法11材料111实验用品LDMEM(Gibco,BRL,USA),胎牛血清(FCS,Gibco,BRL,USA),其它化学用品均购自Sigma公司。多孔β磷酸三钙陶瓷由法国地中海大学卢建熙博士提供,呈圆柱状,长21mm,直径10mm,孔隙率70...
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细胞培养基应用选择
...巢F-12,10%胎牛血清AR42J.大鼠胰腺肿瘤Ham‘sF-12K,20%胎牛血清BRL3A.大鼠肝Coon‘sF-12K,5%胎牛血清Clone9上皮细胞大鼠肝脏F-12K,10%胎牛血清H4--Ⅱ-E-C3上皮样大鼠肝癌F-12K,10%胎牛血清`GH1上皮细胞大鼠垂体肿瘤F-10,15%马血清和2.5%胎牛血清GH3上...
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第十九章 核酸(基因)分子探针--第一节 概述
...素化核酸探针,成功地用于探测一系列基因。随后美国的BRL和Enzobiochem生产了这种生物素标记探针及分子杂交成套试剂盒。1985年澳大利亚合成了光敏生物素,用于直接标记核酸,这是Adelaide大学Forest等学者的重大成功。该大学的B...
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TNF-α诱导类风湿关节炎滑膜成纤维细胞MAPKs通路的活化
...膜组织,立即于无菌条件下剪碎,1.0mg/mlI型胶原酶(Gibco-BRL)37℃消化4h,离心收集细胞,加入20%FBS-DMEM(Gibco-BRL)培养液,置于37℃、5%CO2孵箱中令其贴壁生长,细胞生长至85%汇满时,胰蛋白酶-EDTA消化进行1:3传代。本实验中采用3~5...
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IdentificationofHypertension-RelatedGenesThroughanIntegratedGenomic-TranscriptomicApproach
...oading.Wereverse-transcribedDNA-freetotalRNA(2g)witholigo(dT)primers(Gibco-BRL),SuperscriptIIreversetranscriptase(Gibco-BRL),anddNTP(BoehringerMannheim)in40-uLreactionbuffer(BibcoBRL).WedesignedprimersandprobeswiththePrimerExpress1.0program(PEAppliedBiosystems).ForTaqMananalysis,weusedanAppliedBiosy...
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ProapoptoticEffectsofCaspase-1/Interleukin-ConvertingEnzymeDominateinMyocardialIschemia
...ratagene)wasexpressedinHEK293cellsaftertransfectionwithLipofectamine(Gibco/BRL).StandardcultureconditionswereDulbeccoMinimumEssentialMedium(DMEM)containing4.5mmol/Lglucoseplus1%antibiotic/antimycoticsolution(Gibco/BRL)and10%fetalbovineseruminanatmosphereof5%CO2,95%air.Forhypoxiastudies,standardmediu...
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PeroxisomeProliferator-ActivatedReceptorAgonistsIncreaseNitricOxideSynthaseExpressioninVascularEndothelialCells
...ntificationofhigh-affinitybindingsitesfortheinsulinsensitizerrosiglitazone(BRL-49653)inrodentandhumanadipocytesusingaradioiodinatedligandforperoxisomalproliferator-activatedreceptorgamma.JPharmacolExpTher.1998;284:751–759.XuX,OtsukiM,SumitaniS,SaitoH,KouharaH,KasayamaS.RU486antagonizestheinhibitor...
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N-(Carboxymethyl)lysineDepositionsinIntramyocardialBloodVesselsinHumanandRatAcuteMyocardialInfarction
...-coated,tissue-cultureplatesin“growthmedium“containingmedium-199(Gibco-BRL,Gaithersburg,Md)supplementedwith20%(v/v)fetalbovineserum,2mmol/LL-glutamine,50µg/mLheparin(Sigma),12.5µg/mLECgrowthsupplement(Sigma),and100U/mLpenicillin/streptomycin(Gibco-BRL)undera5%CO2atmosphereat37°C....