-
科学家完成肺孢子菌全基因组测序
...条件下获得P.jirovecii细胞,所以无法获得其基因组DNA,但是Hauser及其同事采取了另外一种方法.他们从肺孢子菌肺炎患者中收集支气管肺泡灌洗液,通过免疫共沉淀的方法收集P.jirovecii细胞,通过随机引物扩增获得混合的DNA双链,这些DNA...
-
Single-Nucleotide-PolymorphismMappingofthePseudomonasaeruginosaTypeIIISecretionToxinsforDevelopmentofaDiagnosticMultiplexPCRSystem
...educedvirulenceinamousemodelofacutelunginfection(11),afindingalsoreportedbyHauseretal.(22).Clinically,isolatesthatsecreteExoUinvitroweremorelikelytohavecausedseverepneumoniainmechanicallyventilatedpatients(21).GiventheevidencethatthetoxinsoftheTTSSareimportantforthedeterminationofstrainvirulenceandp...
-
医生用干细胞制造女性生殖器
...率四千分一两名女子所患的罕见疾病Mayer-VonRokitansky-Kuster-HauserSyndrome(MRKHS),每4,000至5,000名新生女婴,有一人会患有此病。患者一般会拥有正常的乳房、卵巢和子宫,但没有阴道,不能进行性行为或生育。此前,医学界会利用皮...
-
GenotypicStudyofPneumocystisjiroveciiinHumanImmunodeficiencyVirus-PositivePatientsinThailand
...numberofribosomalRNAgenesinPneumocystiscarinii.J.Eukaryot.Microbiol.41:88S.Hauser,P.M.,P.Francioli,J.Bille,A.Telenti,andD.S.Blanc.1997.TypingofPneumocystiscariniif.sp.hominisbysingle-strandconformationpolymorphismoffourgenomicregions.J.Clin.Microbiol.35:3086-3091.Helweg-Larsen,J.,C.H.Lee,S.Jin,J.Y.H...
-
芯片技术在医学实验室应用展望
...量。生物芯片在HIV检测及相关研究中也发挥了一定作用。Hauser等[8]应用DNA芯片技术在艾滋病患者出现抗体反应之前检测艾滋病病毒,对该病的早期诊断意义重大。2.3在肿瘤诊断中的应用肿瘤的发生发展往往涉及到较多基因的...
-
Intraoperativevisualexperiencesofcataractpatientscanbebothpleasantandunpleasant
...dicherry,India4LionsAravindInstituteofCommunityOphthalmology,Modurai,India5Hauser-RossEyeInstitute,Sycamore,IL,USA6AcademicDepartmentofAnaesthesia,TheJamesCookUniversityHospital,UKCorrespondenceto:DrKah-GuanAuEongAlexandraHospital378AlexandraRoadSingapore,Singapore159964;kah_guan_au_eong@alexhosp.co...
-
先天性无阴道
...管细小,卵巢发育及功能正常常为特征的Rokitansky-Kustner-Hauser综合征病人为最多见。睾丸女性化(雄激素不敏感综合征)病人较为少见。很少数为真性两性畸形或性腺发育不全者。【诊断】绝大多数先天性墙角阴道病人在正常阴...
-
先天性无阴道
...管细小,卵巢发育及功能正常常为特征的Rokitansky-Kustner-Hauser综合征病人为最多见。睾丸女性化(雄激素不敏感综合征)病人较为少见。很少数为真性两性畸形或性腺发育不全者。临床表现绝大多数先天性墙角阴道病人在正常阴...
-
先天性无阴道继发阴道外翻一例
...管、卵巢及外生殖器发育正常,称之为Meyer-Rokitausky-Kuster-Hauser-Syndrome(MRKH)综合征。正常情况下女性胚胎发育过程中副中肾管头端发育成输卵管,中、尾端发育成子宫及阴道上段。双侧副中肾管中、尾段发育时相互融合,纵隔消失...
-
基因芯片技术及其在临床检验医学中的应用
...,这也是基因芯片技术被首次应用于临床实践。1998年,Hauser等[7]人应用DNA芯片技术在艾滋病患者出现抗体反应前检测艾滋病病毒,对此病的早期诊断十分重要;同年,Kimizuka[8]与Affymetrix公司合作生产了新一代的诊断试剂盒...
-
癫痫患者95例的临床类型、脑电图及CT分析
...龄与病因的关系本研究中15岁以下儿童癫痫特发性多见,Hauser认为出生时神经缺陷是小儿癫痫的最重要的病因[3];老年人以继发性癫痫为主,其病因以脑血管病最多,其次是脑肿瘤、脑外伤、一氧化碳中毒、代谢性脑病等[4]...
-
肿瘤的抗体治疗
...tastaticbreastcancer.JClinOncol,2002,20:719-726.8DandachiN,DietzeO,Hauser-KronbergerC.Chromogenicinsituhyˉbridization:anovelapproachtoapracticalandsensitivemethodforthedetechionofHER2oncogeneinarchivalhumanbreastcarcinoma.LabInvest,2002,82:1007-1014.9HerbstRS,HongWK.IMC-C225,ananti-...
-
髌骨不稳定的诊治现状
...良好疗效,但术后并发症较高。 (2)髌韧带止点移位术(Hauser法):将髌韧带连同胫骨结节的骨片向远侧和内侧移位固定,通常与外侧松解和股内侧肌前移术联合应用。适用于髌骨脱位复位后伸屈不受影响,高位髌骨,胫骨结节已...
-
InfectionbyChlamydophiliaaviuminanElderlyCoupleWorkinginaPetShop
...001.Chlamydialinfection,p.1075-1084.InE.Braunwald,A.S.Fauci,D.L.Kasper,S.L.Hauser,D.L.Longo,andJ.L.Jameson(ed.),Harrison‘sprinciplesofinternalmedicine,14thedition,vol1.McGraw-Hill,NewYork,N.Y.Wang,S.P.,andJ.T.Grayston.1970.ImmunologicrelationshipbetweengenitalTRIC,lymphogranulomavenereum,andrelate...
-
Blimp-1isrequiredformaintenanceoflong-livedplasmacellsinthebonemarrow
...ofplasmacelldevelopment.Nat.Rev.Immunol.5:230–242.Hoyer,B.F.,K.Moser,A.E.Hauser,A.Peddinghaus,C.Voigt,D.Eilat,A.Radbruch,F.Hiepe,andR.A.Manz.2004.Short-livedplasmablastsandlong-livedplasmacellscontributetochronichumoralautoimmunityinNZB/Wmice.J.Exp.Med.199:1577–1584.Silverman,G.J.,andS.Weisman.2...
-
阴道成形围手术期20例的临床护理分析
...分重要。【关键词】RKH;阴道成形;护理 Rokitansky-Kustner-Hauser综合征:指胚胎在发育期间受到内在或外界因素阻扰,亦可能由于基因突变引起副中肾管发育异常。先天性无阴道的患者通常都具有46XX染色体核型,常合并无子宫或...
-
抑郁症的研究进展
...感反应。抑郁症的发生与额叶功能性和器质性损害有关。Hauser等指出[4],老年抑郁症病人比正常老年人的额叶体积小(220±31)ml∶(250±3.1)ml,P0.01,显示老年抑郁症病人有额叶萎缩的相对特异性,有人用核磁共振(MRI)研...
-
腺病毒载体介导的遗传病基因治疗研究和应用进展
...毒等)载体,将功能性营养蛋白的cDNA导入肌肉细胞内。Hauser等[7]在转基因mdx小鼠中,分别转移6.3kb的一段基因和cDNA全基因,证实可以部分或全部纠正小鼠的病理症状,小鼠的肌肉力量可以恢复。1993年,Vincent等[8]最早通过缺失E1...
-
SelectingBcellsandplasmacellstomemory
...calmemorybypolyclonalactivationofhumanmemoryBcells.Science.298:2199–2202.Hauser,A.E.,G.F.Debes,S.Arce,G.Cassese,A.Hamann,A.Radbruch,andR.A.Manz.2002.ChemotacticresponsivenesstowardligandsforCXCR3andCXCR4isregulatedonplasmablastsduringthetimecourseofamemoryimmuneresponse.J.Immunol.169:1277–1282.Z...
-
CorynebacteriumulceransinanImmunocompromisedPatientwithDiphtheriaandHerDog
...i.2003.CorynebacteriumulceransdiphtheriainJapan.Emerg.Infect.Dis.9:752-753.Hauser,D.,M.R.Popoff,M.Kiredjian,P.Boquet,andF.Bimet.1993.PolymerasechainreactionassayfordiagnosisofpotentiallytoxinogenicCorynebacteriumdiphtheriaestrains:correlationwithADP-ribosylationactivityassay.J.Clin.Microbiol.31:2720...
-
自闭症的病因研究进展
...象,并由此假设颞叶功能障碍可能是一个重要发病因素(Hauser);患者的胼胝体和下后区体积明显小于正常的对照组(Piven);小脑神经元的广泛减少(Williams);小脑发育不良或过度增生(Courchesne);大脑皮质代谢呈弥漫性减弱(Sherman);脑血流灌...
-
DihydropteroateSynthaseandNovelDihydrofolateReductaseGeneMutationsinStrainsofPneumocystisjiroveciifromSouthAfrica
...4.Nahimana,A.,M.Rabodonirina,G.Zanetti,I.Meneau,P.Francioli,J.Bille,andP.M.Hauser.2003.AssociationbetweenaspecificPneumocystisjirovecidihydropteroatesynthasemutationandfailureofpyrimethamine/sulfadoxineprophylaxisinhumanimmunodeficiencyvirus-positiveand-negativepatients.J.Infect.Dis.188:1017-1023.Ri...
-
ImprovementofLymeBorreliosisSerodiagnosisbyaNewlyDevelopedRecombinantImmunoglobulinG(IgG)andIgMLineImmunoblotAssayandAdditionofVlsEandD
...iaburgdorferipathogenicforhumans.Int.J.Med.Microbiol.293(Suppl.37):172-173.Hauser,U.,G.Lehnert,R.Lobentanzer,andB.Wilske.1997.InterpretationcriteriaforstandardizedWesternblotsforthreeEuropeanspeciesofBorreliaburgdorferisensulato.J.Clin.Microbiol.35:1433-1444.Heikkil,T.,I.Seppl,H.Saxen,J.Panelius,H.Y...
-
EarlyImmuneResponsetotheComponentsoftheTypeIIISystemofPseudomonasaeruginosainChildrenwithCysticFibrosis
...sis.Pediatrics100:E2.Feltman,H.,G.Schulert,S.Khan,M.Jain,L.Peterson,andA.R.Hauser.2001.PrevalenceoftypeIIIsecretiongenesinclinicalandenvironmentalisolatesofPseudomonasaeruginosa.Microbiology147:2659-2669.FitzSimmons,S.C.1993.Thechangingepidemiologyofcysticfibrosis.J.Pediatr.122:1-9.Frank,D.W.1997.Th...
-
多胎妊娠
...。2.年龄及产次:年龄对单卵双胎发生率的影响不明显。Hauser等发现单卵双胎发生率在20岁以下妇女为3‰,>40岁者为4.5‰。双卵双胎发生率随年龄的增长显著升高,在15~19岁年龄组仅2.5‰,而30~34岁组上升至11.5‰。产次增加,...
-
AntibodiesfromPatientswithMelioidosisRecognizeBurkholderiamalleibutNotBurkholderiathailandensisAntigensintheIndirectHemagglutinationAssay
...worth,A.Cronin,B.Crossett,P.Davis,D.DeShazer,T.Feltwell,A.Fraser,Z.Hance,H.Hauser,S.Holroyd,K.Jagels,K.E.Keith,M.Maddison,S.Moule,C.Price,M.A.Quail,E.Rabbinowitsch,K.Rutherford,M.Sanders,M.Simmonds,S.Songsivilai,K.Stevens,S.Tumapa,M.Vesaratchavest,S.Whitehead,C.Yeats,B.G.Barrell,P.C.Oyston,andJ.Park...
-
MEF2Asequencevariantsandcoronaryarterydisease:achangeofheart?
...nearly-andlate-settlementpopulationsofFinland.Am.J.Hum.Genet.67::1481-1493.Hauser,E.R.etal.2004..Agenomewidescanforearly-onsetcoronaryarterydiseasein438families:theGENECARDStudy.Am.J.Hum.Genet.75::436-447.Francke,S.etal.2001..Agenome-widescanforcoronaryheartdiseasesuggestsinIndo-Mauritiansasusceptib...
-
美国科学家用人体自身细胞培养出人造阴道
...管细小,卵巢发育及功能正常常为特征的Rokitansky-Kustner-Hauser综合征病人为最多见。睾丸女性化(雄激素不敏感综合征)病人较为少见。很少数为真性两性畸形或性腺发育不全者。临床表现1、青春期前无明显异常表现,常被忽视...
-
FatalReactivationofPostnatalCytomegalovirusInfectionwithRapidEmergenceofGanciclovirResistanceinanInfantafterAllogeneicStemCellTransplantati
...L97protein.J.Med.Virol.65:70-76.Michel,D.,K.Wunderlich,M.Michel,T.Wasner,I.Hauser,M.Just,W.Hampl,andT.Mertens.2003.Fastgenotypicidentificationandestimationofganciclovir-resistantcytomegalovirusfromclinicalspecimens.Monogr.Virol.24:160-170.Pinkel,D.1998.Differentiatingjuvenilemyelomonocyticleukemiafr...
-
VariationsinemmTypeamongGroupAStreptococcalIsolatesCausingInvasiveorNoninvasiveInfectionsinaNationwideStudy
...ealisolatesandtheirrelationshiptoinvasiveisolates.J.Infect.Dis.185:915-920.Hauser,A.R.,D.L.Stevens,E.L.Kaplan,andP.M.Schlievert.1991.MolecularanalysisofpyrogenicexotoxinsfromStreptococcuspyogenesisolatesassociatedwithtoxicshock-likesyndrome.J.Clin.Microbiol.29:1562-1567.Helweg-Larsen,J.,J.S.Jensen,T...