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点突变p53肽诱导肽特异性CTL的研究
...ontNEN,美国)。 4.肽合成:P132F肽(LNKLFFQL)和仙台病毒肽SEV-9(FAPGNYPAL,作为对照肽)的合成见参考文献〔2〕。 5.肽免疫、CTL诱导及其杀伤活性测定:肽与佐剂(IFA或RAS)充分混匀,给C57BL/6小鼠免疫(尾根部皮下注射,免疫剂量为100...
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北大、武大《PNAS》文章介绍MDA5的一种生理抑制剂
...或TLR3介导的IFN-Β感应。DAK过量表达还会抑制细胞质dsRNA和SeV诱导的IFN-β启动子的活化;而RNAi沉默内源性DAK后,IFN-β启动子活化,dsRNA或者Sev激发的INF-β的活化水平上升。另外,DAK的过量表达会抑制MDA5但不会抑制RIG-I介导的抗病毒...
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Alcoholintakeinrelationtobrainmagneticresonanceimagingfindingsinolderpersonswithoutdementia
...fmanandMoniqueMBBreteler1FromtheDepartmentsofEpidemiologyBiostatistics(TdH,SEV,EjvD,NDP,CMvD,AH,andMMMB)andofNeurology(TdH,SEV,EjvD,NDP,andPJK),ErasmusMedicalCenter,Rotterdam,Netherlands.2SupportedbytheNetherlandsOrganizationforScientificResearch(NWOgrant904-61-096),theNetherlandsHeartFoundation(gra...
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AIRE研究
...tients,aged18years,withevidenceofheartfailure3–10daysafterMI;patientswithsevereheartfailure(usuallyNYHAclassIV)orongoingischemiaexcludedFollowupandprimaryendpointAverage15monthsfollowup.Primaryendpointall-causemortalityTreatmentPlaceboorramiprilinitiatedat2.5mgtwicedaily;increasedto5mgtwicedailyaf...
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隔蛋白7逆转裸鼠移植胶质瘤的恶性表型
...TJ905细胞的裸鼠皮下移植瘤模型,将荷瘤鼠分为治疗组(SEVIT/pcDNA3)、空载体组(pcDNA3)和对照组(PBS),观察各组肿瘤的大小及生长速度;采用免疫组化法检测各组肿瘤组织标本中SEPT7、增殖细胞核抗原(PCNA)、胶质纤维酸...
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武汉病毒所天然免疫通路调控研究取得进展
...P抑制MITA介导的I型干扰素信号通路,即使存在仙台病毒(SeV)和细菌第二信号分子(c-di-GMP)的刺激过表达MRP仍可以剂量依赖的抑制MITA介导的天然免疫通路。后期的研究表明MRP的抑制机制主要是通过和MITA作用从而影响MITA和TBK1的...
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NonendothelialMesenchymalCell-DerivedMCP-1IsRequiredforFGF-2-MediatedTherapeuticNeovascularization
...ctor)andarteriogenic(MCP-1)signalsviaindependentsignaltransductionpathways.SeverehindlimbischemiastimulatedMCP-1expressionthatwasstronglyenhancedbyFGF-2genetransfer,andablockadeofMCP-1activityviaadominantnegativemutantaswellasadeficiencyofitsfunctionalreceptorCCR2resultedinthediminishedrecoveryofblo...
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Cognitiveperformanceamongtheelderlyanddietaryfishintake:theHordalandHealthStudy
...slo,Norway(EN,CAD,HRandKS);theDepartmentofPublicHealthandPrimaryHealthCare(SEV,HAN,andGST)andtheInstituteofMedicine(ON),UniversityofBergen,Bergen,Norway;andtheDepartmentofGeriatricMedicine,NorwegianCentreforDementiaResearch,UllevålUniversityHospital,Oslo,Norway(KE)Seecorrespondingeditorialonpa...
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新病毒诱导I型干扰素负性调控机制
...头蛋白VISA的相互作用,从而抑制由仙台病毒(SendaiVirus,SeV)引起的IRF3入核以及I型干扰素的产生。在HCV慢性感染的病人外周血细胞中,发现TBK1s的表达高低与HCV慢性感染相关联。本研究成果对进一步丰富I型干扰素产生的调控网络...
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昆明动物所揭示新型HIV病毒限制因子TRIMCyp的起源和功能
...成的。有趣的是,树鼩TRIMCyp而非TRIM5-4能负调控仙台病毒(SeV)介导的抗病毒天然免疫应答,下调NF-κB和IFN-β的表达,提示树鼩TRIMCyp丧失了HIV病毒限制功能而产生了新的免疫调控功能。该研究不仅拓展了病毒限制因子TRIM5/TRIMCyp的功...
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Incidenceofkeratitisofvaryingseverityamongcontactlenswearers
...Acceptedforpublication29August2004ABSTRACTAim:Todeterminetheincidenceofnon-severekeratitis(NSK)andseverekeratitis(SK)amongwearersofcurrentgenerationcontactlenses.Methods:A12month,prospective,hospitalbasedepidemiologicalstudywasconductedbyexaminingallcontactlenswearerspresentingwithacornealinfiltrate...
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β1整合素表达下调对人结肠癌细胞增殖和化疗敏感性影响
...ta;两个亚基组成的异二聚体,β亚基的胞浆区含有Asp-X-Sev-X-Sev序列,参与细胞骨架蛋白的相互作用和胞内信号传导。整合素将胞外的ECM分子与细胞内的骨架蛋白连接起来形成焦点黏附物。焦点黏附物是整合素内外双向信号传导...
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PlasmavitaminB-6formsandtheirrelationtotranssulfurationmetabolitesinalarge,population-basedstudy
...ysteineandRelatedVitamins,UniversityofBergen,Bergen,Norway(ØM,SH,JS,SEV,andPMU);theHormoneLaboratory,HaukelandUniversityHospital,Bergen,Norway(SH);theSectionforPharmacology,InstituteofMedicine,UniversityofBergen,andHaukelandUniversityHospital,Bergen,Norway(SH);andtheDepartmentofBiomedicalScie...
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MolecularPhenotypesofAtherosclerosis
...ncedlesionisdesirable.Thepresenceatautopsyofatheroscleroticlesionsofvaryingseverityandclassifications1withinthesameindividualreflectsthecomplexityofthischronicinflammatoryprocess.Furthermore,certainarteriallocationsarepredictiveforlesionformationbeforeanydetectablepathologicalchangeisapparent,wherea...
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Patternsandpredictorsoffolicacidsupplementuseamongpregnantwomen:theNorwegianMotherandChildCohortStudy
...ologyandMedicalStatistics,DepartmentofPublicHealthandPrimaryHealthCare(RMN,SEV,andHKG),andtheLOCUSforHomocysteineandRelatedVitaminsandtheSectionforPharmacology,InstituteofMedicine(PMU),UniversityofBergen,Bergen,Norway;theMedicalBirthRegistry,NorwegianInstituteofPublicHealth,Bergen,Norway(SEV);andthe...
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RelationsofglutamatecarboxypeptidaseII(GCPII)polymorphismstofolateandhomocysteineconcentrationsandtoscoresofcognition,anxiety,anddepression
...ord,UnitedKingdom(HRandADS);andtheUniversityofBergen,Bergen,Norway(OKN,PMU,SEV,andGST)2HRiscurrentlyattheDepartmentofNutrition,InstituteofBasicMedicalSciences,UniversityofOslo,Oslo,Norway.3SupportedbyresearchgrantsDK56085andDK35747fromtheUSNationalInstitutesofHealthtoCHHandbyagrantfromtheNormanColli...
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Dietaryfatandplasmatotalhomocysteineconcentrationsin2adultagegroups:theHordalandHomocysteineStudy
...ay(PB,HR,EN,CAD,andGU);theDepartmentofPublicHealthandPrimaryHealthCare(SVK,SEV,andGST)andtheSectionforPharmacology,InstituteofMedicine(HRandPMU),UniversityofBergen,Norway;theOxfordCentreforGeneFunction,DepartmentofPhysiology,AnatomyGenetics,UniversityofOxford,UnitedKingdom(HR);andtheDepartmentofPrev...
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DetectionofHumanInfluenzaAVirusesbyLoop-MediatedIsothermalAmplification
....Earlyidentificationofthevirusisthereforecriticalforthecontrolofthedisease.Severalmoleculartechniqueshavebeendevelopedasrapidtestsforclinicaldiagnosisofinfluenzavirus(1,10).Therecentinventionofloop-mediatedisothermalamplification(LAMP)providesanewalternativeformoleculardiagnosis(8).Oneofthemajoradva...
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Bodymassindexandfatmassaretheprimarycorrelatesofinsulinresistanceinnondiabeticstage3–4chronickidneydiseasepatients
...donESRDpatients,butanincreasingrecognitionofthehighprevalenceofmoderate-to-severeCKDhasredirectedtheattentiontothispatientpopulationtoidentifyriskfactorsassociatedwithhospitalization,death,andprogressiontoESRD.Indeed,studieshaveshownthatthereisagreaterriskofatheroscleroticeventsandahigherriskofdeath...
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Changesinlifestyleandplasmatotalhomocysteine:theHordalandHomocysteineStudy
...dPerMUeland1FromtheDepartmentsofPublicHealthandPrimaryHealthCare(EN,GST,andSEV)andPharmacology(HRandPMU),theInstituteofMedicine(ON),andtheLocusforHomocysteineandRelatedVitamins(EN,GST,SEV,ON,andPMU),theUniversityofBergen,Norway;theDepartmentofPharmacology,UniversityofOxford,UnitedKingdom(HR);andtheM...
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ThemetabolicsyndromeinadultsprenatallyexposedtotheDutchfamine
...49;prenatalfaminebirthweightINTRODUCTIONThemetabolicsyndromeisknownbyseveralnames:syndromeX,thedeadlyquartet,andtheinsulinresistancesyndrome(1-3).Itisaconstellationofinterrelatedmetabolicriskfactorsthatpredisposesapersontodeveloptype2diabetesandcardiovasculardisease(4,5).Clinicalmanifestations...
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ApplicationofaMolecularPanelToDemonstrateEnterotropicVirusSheddingbyHealthyandHumanImmunodeficiencyVirus-InfectedPatients
...Department,VastoHospital,Vasto,ItalyABSTRACTWeusedamolecularpanel,targetingsevenentericviruses,toexploretheadvantageofusingmolecularmethodstoestablishtheetiologyofentericdiseasesandtoevaluatetheprevalenceofentericvirusesinasymptomatichumanimmunodeficiencyvirus-infectedpatients.Thisapproachfavorsrapi...
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EssentialroleofIPS-1ininnateimmuneresponsesagainstRNAviruses
...hecriticalroleofIPS-1inantiviralresponsesinvivo.IPS-1–deficientmiceshowedseveredefectsinbothRIG-I–andMda5-mediatedinductionoftypeIinterferonandinflammatorycytokinesandweresusceptibletoRNAvirusinfection.RNAvirus–inducedinterferonregulatoryfactor-3andnuclearfactorBactivationwasalsoimpairedinIPS-...
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DevelopmentofaTriplexReal-TimePCRAssayforDetectionofPanton-ValentineLeukocidinToxinGenesinClinicalIsolatesofMethicillin-ResistantStaphyloco
...n2002(8).Recently,MRSAstrainsproducingPVLhavebeenassociatedwithanincreaseinsevereskininfectionsandanewsyndromeofcommunity-associatednecrotizingpneumoniainchildren(19).ThePVLgenesarecarriedonthephageSLT(15),buttodate,PVLhasnotbeenidentifiedwithepidemicorendemichospital-acquiredMRSAstrains(12).Withthe...
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脑脊液鼻漏致复发性化脓性脑膜炎的外科诊治探讨
...m1982to2004werereviewed.Allofcasesunderwentsurgicaltreatment.ResultsThirty-sev-enoperationsweredoneon25cases.ThetotalsuccessrateofclosureofCSFrhinorrheawas92%.Elevencasesweretreatedbyneurosurgeon.Ofthe11cases,9receivedrepairsurgeryinextracranialapproachesthroughsphenoidsinus,and2casesinextradura...
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Plasmaconcentrationoffolateasabiomarkerfortheintakeoffruitandvegetables:theHordalandHomocysteineStudy
...ofOslo(AB,EBL,CAD,andLFA);theDepartmentsofPublicHealthandPrimaryHealthCare(SEVandGST)andPharmacology(HRandPMU),UniversityofBergen,Bergen,Norway;andtheDepartmentofPharmacology,UniversityofOxford,Oxford,UnitedKingdom(HR)2SupportedbytheNorwegianInstituteofPublicHealth,TheCommunityHealthServiceofHordala...
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InhaledCorticosteroidsandtheBeneficialEffectofDeepInspirationinAsthma
...orticosteroidsonthesephenomena.Twogroupsofsubjectswithasthma,9withmoderate/severehyperresponsivenesstomethacholine,and12withmild/borderlinehyperresponsivenesstomethacholine,receivedinhaledfluticasone(880edaily)for12weeks.SerialbronchoprovocationswereperformedatWeeks0,6,and12.Theimpactofdeepinspirati...
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保留喉功能下咽癌手术切除与修复
...tsThe3-yearsurvivalratewas73.9%(17/23)and5-yearsurvivalratewas47.8%(11/23).Severecomplicationoccurredin8of23(34.8%).Completerestorationoflaryngealfunctionwasachievedin20cases(87%)anddeglutitionrestoredinallpatients(100%).Themeandurationoforalintakewas11.8days(6~33days)andmeanhospitalizationwas32da...
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AssociationBetweenCarotidPlaqueCharacteristicsandSubsequentIschemicCerebrovascularEvents
...ompositionsuchasthesizeofthelipidcoreandpresenceofintraplaquehemorrhage.1,2Severalprospectivestudiesusingultrasoundhaveshownthatecholucentcarotidplaquesareassociatedwithincreasedriskforcerebrovascularevents.3–6Echolucentplaquesareknowntohaveahighercontentoflipidandhemorrhagethandoechogenicplaques,...
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Paediatriccataractblindnessinthedevelopingworld:surgicaltechniquesandintraocularlensesinthenewmillennium
...edtoavoidnystagmusandalifetimeofpoorvisualacuity. Currently,approximatelysevenmillionadultcataractsurgeriesareperformedannuallyintheworld.15Precisedatarelatedtothetotalnumberofpaediatriccataractsurgeriesperformedannuallyarenotavailable.However,paediatriccataractblindnesspresentsanenormousproblemto...