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CriticalfunctionofBmx/Etkinischemia-mediatedarteriogenesisandangiogenesis
...constitutivelyactiveformofBmxundertheendothelialTie-2enhancer/promoter(Bmx-SK-Tgmice)tostudytheroleofBmxinischemia-mediatedarteriogenesis/angiogenesis.Inresponsetoischemia,Bmx-KOmicehadmarkedlyreduced,whereasBmx-SK-Tgmicehadenhanced,clinicalrecovery,limbperfusion,andischemicreservecapacitywhencompar...
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UseofPhylogeneticandPhenotypicAnalysesToIdentifyNonhemolyticStreptococciIsolatedfromBacteremicPatients
...rposes,24typeand9referencestrainswereanalyzed:S.sanguinisstrainsATCC10556T/SK1,SK4,andSK36(S.sanguinisstrainwhosegenomeiscurrentlybeingsequencedatVirginiaCommonwealthUniversity;www.sanguinis.mic.vcu.edu);S.oralisstrainsNCTC11427T/SK23andSK34;S.gordoniistrainsATCC10558T/SK3andChallis/SK7;S.mitisstrai...
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RenalexpressionandactivityofthegerminalcenterkinaseSK
...inkinases.ThisscreeningdemonstratedexpressionofaproteinkinaseconsistentwithSK2,agroupIIgerminalcenterkinaseandhomologofhumanSte20-likekinase(SLK).SK2mRNA,proteinexpression,andkinaseactivitywereincreasedinratfetalkidneyhomogenates(embryonicdays17-21)comparedwithadultcontrols.Inadultkidneyssubjectedto...
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载脂蛋白E在神经细胞中表达对细胞功能的影响
...时,探讨apoE4在AD发病中的相关机制。研究方法筛选转染SK-N-SH定位观察表达鉴定功能分析构建真核表达质粒重组pEGFP-apoE质粒酶切电泳图apoE3Query:321cgcaggcccggctgggcgcggacatggaggacgtgtgcggccgcctggtgcagtaccgcg380||||||||||||||||||||||||||||||||||||||||||||||...
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CentralEuropeanDobravaHantavirusIsolatefromaStripedFieldMouse(Apodemusagrarius)
InstituteofVirology,HelmutRuskaHaus,ChariteSchoolofMedicine,HumboldtUniversity,D-10098Berlin,GermanyInstituteofVirologyInstituteofZoology,SlovakAcademyofSciences,84206Bratislava,SlovakiaABSTRACTDobravavirus(DOBV)isahantavirusthatcauseshemorrhagicfeverwithrenalsyndrome(HFRS)inEurope.Itishostedbyatlea...
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InhibitoryGatingModulationofSmallConductanceCa2+-ActivatedK+ChannelsbytheSyntheticCompound(R)-N-(Benzimidazol-2-yl)-1,2,3,4-tetrahydro-1-naphtyla
【关键词】InhibitoryGatingModulationSKchannelsaresmallconductanceCa2+-activatedK+channelsimportantforthecontrolofneuronalexcitability,thefinetuningoffiringpatterns,andtheregulationofsynapticmechanisms.TheclassicSKchannelpharmacologyhaslargelyfocusedonthepeptideapamin,whichactsextracellularlybya...
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Bmi-IsEssentialfortheTumorigenicityofNeuroblastomaCells
...eeoftheMedicalUniversityofOhio.CellCultureHumanneuroblastomacelllinesSHEP1,SK-N-AS,SK-N-F1,SK-N-DZ,andSK-N-SHweregrowninDulbecco??smodifiedEagle??smediumsupplementedwith10%fetalbovineserum(FBS);IMR32,SK-N-BE(2),BE(2)-C,SY5Y,andLA1C55Nina1:1mixtureofDulbecco??smodifiedEagle??smediumandHam??snutrientm...
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Incidenceofkeratitisofvaryingseverityamongcontactlenswearers
...ation29August2004ABSTRACTAim:Todeterminetheincidenceofnon-severekeratitis(NSK)andseverekeratitis(SK)amongwearersofcurrentgenerationcontactlenses.Methods:A12month,prospective,hospitalbasedepidemiologicalstudywasconductedbyexaminingallcontactlenswearerspresentingwithacornealinfiltrate/ulcertoahospital...
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ReceptorRegulationofGeneExpressionofAxonGuidanceMolecules:ImplicationsforAdaptation
...s)andmodulationofaxonguidancemolecules,weinvestigatedwhetherGPCRactivityinaSK-N-MChumanneuroepitheliomacellline(whichexpresseslowlevelsofD1dopaminereceptors)affectsgeneexpressionofaxonguidancemolecules(semaphorins,ephrins,netrins,andtheirreceptors).Usingreal-timepolymerasechainreaction,weidentified1...
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小剂量重组织型纤溶酶原激活剂与尿激酶、重组链激酶溶栓治疗急性心肌梗死的临床对比分析
...纤溶酶原激活剂(rt-PA)、尿激酶(UK)和重组链激酶(r-SK)治疗急性心肌梗死的疗效和安全性。方法114例急性心肌梗死患者随机分为rt-PA组38例,UK组37例,r-SK组39例。分别应用纤溶酶原激活剂50mg、尿激酶150万u、链激酶150万u静...
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EndothelialNitricOxideSynthaseActivationbyTumorNecrosisFactorThroughNeutralSphingomyelinase2,SphingosineKinase1,andSphingosine1PhosphateRe
...ialactivationofneutral-sphingomyelinase-2(N-SMase2)andsphingosine-kinase-1(SK1)andgenerationofsphingosine-1-phosphate(Sph1P).InhibitionofN-SMase2inhibitedSph1Pformation,whereasinhibitionofSK1didnotaffectN-SMase2activationbyTNF-.BlockadeofN-SMase2,SK1,ortheSph1PreceptorsS1P1andS1P3,eitherbysilencingo...
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Depressionandcardiovascularmortality:aroleforn3fattyacids?
...sandPrimaryCare,UniversityMedicalCenterUtrecht,Utrecht,Netherlands(MHK,MIG,SK,andDEG);theCentreforPreventionandHealthServicesResearch,NationalInstituteforPublicHealthandtheEnvironment,Bilthoven,Netherlands(MART);andtheDivisionofHumanNutrition,WageningenUniversity,Wageningen,Netherlands(DK)2MarjaARTi...
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EvidenceforInvolvementofBothIKCaandSKCaChannelsinHyperpolarizingResponsesoftheRatMiddleCerebralArtery
...ngIKCachannelsalone,contrastingwithperipheralvesselswhereblockofbothIKCaandSKCaisrequired.AsthecontributionofIKCaandSKCatoendothelium-dependenthyperpolarizationdiffersinperipheralarteries,dependingonthelevelofarterialconstriction,weinvestigatedthepossibilitythatSKCamightcontributetoequivalenthyperpo...
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NeuroprotectiveEffectsof17-EstradiolandNonfeminizingEstrogensagainstH2O2ToxicityinHumanNeuroblastomaSK-N-SHCells
...tiveagents.EpidemiologicalstudiesshowthatearlyestrogentherapycanreducetheriskofneurodegenerativediseasessuchasAlzheimer‘sdiseaseandimprovecognitionandmemoryinADpatients(Hendersonetal.,1994;Birge,1996;Sherwin,1996;Tangetal.,1996;Yaffeetal.,1998;Costaetal.,1999;Baggeretal.,2005).Inaddition,estrogent...
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TheH+-LinkedMonocarboxylateTransporter(MCT1/SLC16A1):APotentialTherapeuticTargetforHigh-RiskNeuroblastoma
...T1mRNAlevelsinfreshneuroblastomabiopsysamplesthatcorrelatedpositivelywithriskoffataldiseaseandamplificationofthe“proto-oncogenic“transcriptionfactorMYCN.WefurtherinvestigatedMCTasapotentialtherapeutictargetinvitro.TheneuroblastomacelllinesevaluatedwereSk-N-SH,CHP134,IMR32,andNGP.Alllinesexhibite...
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IronChelationbyClinicallyRelevantAnthracyclines:AlterationinExpressionofIron-RegulatedGenesandAtypicalChangesinIntracellularIronDistribution
...ithDOXcouldnotprotectcellsfromthetoxicityofsubsequentironloadingbyFAC.HumanSK-Mel-28melanomacellsorratH9c2cardiomyocyteswerepreincubatedwithCONor5µMDOXfor24hat37°C,andthentheywerewashed.Thecellswerethenreincubatedfor16hat37°CwithCONcontainingincreasingconcentrationsofFAC(250,500,750,an...
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脱水蛋白在逆境下的分子作用机制研究进展
...结构保守性强,具有K,S和Y等3个高度保守区域,通常以YSK的顺序结合在一起,中间夹有低保守性、高重复性的φ片段。Y片段为(V/T)DEYGNP,位于N末端,通常有1—3个串连拷贝,推测与植物和细菌中蛋白的核定位有关口;K片段(EKKGIMD...
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ROMKisrequiredforexpressionofthe70-pSKchannelinthethickascendinglimb
...ceexhibitaBartterphenotypeandlackthesmall-conductance(30-pS)apicalKchannel(SK)intheTAL.However,adistinct70-pSKchannelcanalsosignificantlycontributetotheapicalconductanceofTAL.WenowexaminetheeffectofROMKdeletiononthefunctionalexpressionofthe70-pSKchannelintheTAL.Functionalexpressionofthe70-pSKchannel...
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胶质瘤患者树突状细胞在裸鼠体内外诱导抗肿瘤免疫
...来自辽宁省锦州市中心医院临床确诊病人;胶质瘤细胞株SK-MG-1购自北京医科大学;SGC-7901人胃癌细胞株、BeL-7402人肝癌细胞株购自中山大学。4周龄BALB/C裸鼠购自北京医科大学。1.2方法1.2.1制备冻融抗原取107个SK-MG-1胶质瘤细胞,加...
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老年急性心肌梗死溶栓治疗对左心室功能的改善作用
...[1,2],对老年急性心肌梗死(AMI)患者应用链激酶(SK)溶栓治疗,能明显降低AMI的病死率,但其对心功能的影响研究尚少。我们研究了2002年2月~2003年6月初次AMI接受SK静脉溶栓治疗的老年患者29例,探讨溶栓再灌注对左心室...
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患肢动脉内注射r-SK对下肢深静脉血栓形成的效果
[摘要]目的探讨患肢动脉注射重组链激酶(r-SK)治疗下肢深静脉血栓形成的安全性和有效性。方法对31例下肢深静脉血栓形成病人,从健侧股动脉穿刺插管至患侧髂股动脉内。r-SK经下肢动脉注入,并动脉内保留导管,每日溶...
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SK能源涉足海藻类生物能开发
近日,SK能源宣布即将与釜山釜庆大学签订协议,正式涉足海藻类生物能的开发。 通过本次协议,SK能源与釜庆大学决定为确保海藻类生物的数量进行相关的技术开发和合作研究。SK能源相关者表示,釜庆大学拥有海藻类的相...
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Phosphorylation-dependenttranslocationofsphingosinekinasetotheplasmamembranedrivesitsoncogenicsignalling
...tofMedicine,UniversityofAdelaide,AdelaideSA5000,AustraliaSphingosinekinase(SK)1catalyzestheformationofthebioactivelipidsphingosine1-phosphate,andhasbeenimplicatedinseveralbiologicalprocessesinmammaliancells,includingenhancedproliferation,inhibitionofapoptosis,andoncogenesis.HumanSK(hSK)1possesseshig...
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SensitivityandspecificityofscanninglaserpolarimetryusingtheGDx
...s.TheGDxprintoutsofallsubjectswereevaluatedbythreeindependentobserversinamaskedfashionandwithouttheclinicalpictureoftheopticdisc.Twoofthethreeobservers(SK,UH)wereGDxexperts,one(KUL)wasanuntrainedGDxuser.Results:AmongtheGDxexperts,sensitivity/specificitywas100%/100%(SK)and90%/100%(UH)indetectingadvan...
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第三节 纤维蛋白溶解药
...药的工作正在进行。 链激酶 链激酶(streptokinase,SK)是C组β溶血性链球菌产生的一种蛋白质,能与纤溶酶原结合,形成SK-纤溶酶原复合物后,促使游离的纤溶酶原转变成纤溶酶,溶解纤维蛋白。因此,需选合适剂量以发...
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急性心肌梗死溶栓药物的研发和临床评价
...剂量可能为150万IU左右,尿激酶治疗AMI有效[3]。2链激酶(SK)链激酶是一种蛋白质,由C组b溶血性链球菌的培养液提纯精制而得,分子量为47kD,血浆半衰期18-33分钟,血池衰减率每小时20%。SK不直接激活纤溶酶原,而是通过与纤溶...
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国产重组链激酶治疗急性心肌梗死临床分析
...在基层医院。常用的溶栓药物有尿激酶(UK)、链激酶(SK)和组织型纤溶酶原激活剂(rt-PA),但各自的价格和再通率不完全相同,选择一种价位适中,再通率高的溶栓剂尤其重要,本文将对国产尿激酶(UK)及国产重组链激酶...
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SK计划开发生物基丁二烯技术
韩国SK创新公司和低碳燃料生产商朗泽科技近日宣布,双方正合作开发生物基1,3-丁二烯的生产技术。此次合作将加快丁二烯替代生产路线的商业化进程。受美国页岩气革命大获成功的影响,丁二烯正变得日益稀缺。多年来,SK在...
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重组链激酶治疗下肢深静脉血栓形成的疗效观察
【摘要】目的探讨重组链激酶(r-SK)治疗下肢深静脉血栓形成的疗效。方法选取2003年5月~12月湘雅医院收治的38例深静脉血栓形成的病人,按入院先后随机单盲分为两组,试验组(重组链激酶组)20例,采用重组链激酶50万U(思...
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Down-RegulationofInhibitorofApoptosisProteinsbyDeguelinSelectivelyInducesApoptosisinBreastCancerCells
...breastcancerandnormalcells.CellLinesAndMaterials.HumanbreastcancercelllinesSK-BR-3andMCF-7andnormalimmortalizedhumanmammaryepithelialcelllineMCF10AwereobtainedfromtheAmericanTypeCultureCollection(Manassas,VA)andculturedinmediumaccordingtotheirrecommendations.Theprimarynormalhumandermalfibroblastcell...