-
实时荧光定量PCR法检测人骨肉瘤耐MTX细胞系中RFC、DHFR、GST-π的mRNA表达
【摘要】[目的]研究RFC(还原叶酸载体)、GST-π(谷胱甘肽S转移酶π)、DHFR(二氢叶酸还原酶)mRNA在人骨肉瘤U2-OS细胞系及人骨肉瘤耐药细胞系U2-OS/R1-R3中的表达差异,并探讨其在人骨肉瘤MTX化疗耐药中的意义。[方法]采用冲...
-
昆明动物所叶酸代谢与神经嵴发育研究取得新进展
...课题组的博士研究生李杰晶等人发现,还原性叶酸载体(RFC)在非洲爪蛙神经嵴发育中具有重要功能。RFC在非洲爪蛙神经嵴组织中特异性表达,干扰RFC的功能会抑制神经嵴的发生,而过表达RFC或注射5-甲基四氢叶酸可以促进神经...
-
中医药逆转类风湿多药耐药思路
...进入细胞通过两种途径:叶酸盐受体和还原叶酸盐载体(RFC)。叶酸盐受体对叶酸的亲和力比对还原叶酸的亲和力要高,RFC对还原叶酸的亲和力相对较高。甲氨喋呤因其结构与生理性叶酸盐相同,主要通过RFC转运入细胞内,因此...
-
Interactionsamongpolymorphismsinfolate-metabolizinggenesandserumtotalhomocysteineconcentrationsinahealthyelderlypopulation
...izinggenes:methylenetetrahydrofolatereductase(MTHFR),reducedfolatecarrier1(RFC1),andglutamatecarboxypeptidaseII(GCPII).Design:Healthyvolunteers(436menand606women;meanage:77.9y)wererandomlyselectedfromamongresidentsofOxford,UnitedKingdom.WedeterminedtheindividualeffectsandinteractionsoftheMTHFR677CT,...
-
妊娠滋养细胞疾病的实验研究进展
...19]应用基因芯片技术,发现复制因子C(replicationfactorC,RFC)在葡萄胎组织和绒癌中表达水平显著高于正常胎盘组织和绒毛细胞。结合免疫组化SP法检测正常绒毛、葡萄胎、侵蚀性葡萄胎和绒癌组织中RFC2和PCNA(增殖细胞核抗原...
-
Homocysteineconcentrationsinadultswithtrisomy21:effectofBvitaminsandgeneticpolymorphisms
...homocysteinepolymorphismCBSMTRMTRRMTHFRRFCvitaminB-12vitaminB-6INTRODUCTIONDownsyndrome(MIM190685),ortrisomy21,isacomplexmetabolicandgeneticdisorderthatstemsfromthefailureofchromosome21tosegregatenormallyduringmeiosis(1).Morethan80clinicalfeatureshaveb...
-
雷贝拉唑三联方案治疗Hp阳性胃炎96例
...法对照组Hp阳性胃炎患者服用雷贝拉唑为主新三联方案即RFC方案(雷贝拉唑10mg、呋喃唑酮0.1g、克拉霉素500mg,均每日2次)共7天;对照组采用国产奥美拉唑为主的三联方案,即OMA方案(奥美拉唑20mg、甲硝唑0.4g、阿莫西林1.0g,均每日2...
-
TheReducedFolateCarrierGeneIsaNovelSelectableMarkerforRecombinantProteinOverexpression
...olateauxotrophsthattakeupfolatevitaminsprimarilyviathereducedfolatecarrier(RFC).Inthisstudy,weshowedthatthehumanRFC(hRFC)genecanserveasanovelselectablemarkerfortheoverproductionofrecombinantproteins.Towardthisend,ahemagglutinin(HA)epitopetaggedhRFC(hRFC-HA)wasintroducedintoabicistronicvector(pIRES2-...
-
杨宝峰校长会见韩国代表团
...峰校长受孙尧副省长委托亲切会见了韩国RNLBIO株式会社和RFC三味食品研究所代表团一行六人,省医学科学院金连弘院长、袁重胜副院长、省外事办韩朝处杨鸿鹏处长等陪同会见。杨校长和金院长向客人详尽地介绍了我校的历史、...
-
维生素B1缺乏是造成糖尿病人血管损伤的原因
...酶来间接测量维生素B1浓度,而这一酶的活性由于THTR-1和RFC-1蛋白维持正常,因此科学家一直没有发现维生素B1的下降。科学家发现血管细胞中维生素B1的减少和微血管并发症密切相关,这似乎和内皮细胞有关,并且会使得患...
-
2008年度“中国高等学校十大科技进展”揭晓
...布) ⑤清华大学 下一代互联网核心技术国际标准RFC5210 (IPv6真实源地址体系结构及测试床) 当前互联网体系结构对分组的源地址不进行验证,带来了网络安全、管理和计费等多方面的问题。清华大学吴建平团队...
-
骨肉瘤治疗的研究进展
...后不良的主要原因,运输MTX跨膜入胞的还原性叶酸载体(RFC)成为肿瘤耐药的新视点。Hattinger等[8]在MTX耐药的变种Saos-2细胞系研究中发现RFC基因表达显著减小,并指出RFC基因表达量与MTX耐药程度有相关性。 2手术治疗 随着化...
-
复方肝必治治疗慢性乙型肝炎高球蛋白血症110例临床观察
...反相血凝法)、抗-HBc、抗-HBe(酶联法)、E-玫瑰花结(E-RFC)、IgG、IgM和IgA。1.4疗效判断显效:主要症状消失,肝、脾、肿大相应回缩,血清总胆红素(TBil)、白蛋白(A)、球蛋白(G)、谷丙转氨酶(ALT)恢复至正常范围。有...
-
He-Ne激光治疗慢性单纯性鼻炎的机制研究
...血红蛋白、血小板、嗜酸性粒细胞、IgG、IgA、IgM、C3、A-RFc、T-RFc和LCT。1.2.3脑血流图测量每例照射一疗程前后的脑血流图。 1.2.4抑菌试验从50例实验对象中任选10例作鼻腔分泌物培养,获金黄色葡萄球菌、溶血性链球菌和大肠...
-
武汉大学生命科学学院细胞分裂新发现登国际刊物
...国家重点实验室的研究人员发表了题为“ReplicationfactorC1(RFC1)isrequiredfordouble-strandbreakrepairduringmeiotichomologousrecombinationinArabidopsis”的文章,发现了一种基因:AtRFC1能通过影响同源染色体和姐妹染色单体的均等分离,调控花粉遗传物...
-
Thesinglenucleotidepolymorphism(80GA)ofreducedfolatecarriergeneintrisomy
...e;hautJeromeLejeuneInstituteParisFranceDearSir:Thereducedfolatecarriergene(RFC1orSLC19A1)locatedonchromosome21(21q22.3)codesforthereducedfolatecarrier,whichisresponsiblefor5-methyltetyrahydrofolateinternalizationwithincells.Wepublishedearlierthe80GAsingle-nucleotidepolymorphism(SNP)inhumanreducedfol...
-
Effectoffolateoversupplementationonfolateuptakebyhumanintestinalandrenalepithelialcells
...gnificantdecreaseintheproteinandmRNAlevelsofthehumanreduced-folatecarrier(hRFC)andadecreaseintheactivityofthehRFCpromoter.ItwasalsoassociatedwithadecreaseinmRNAlevelsoftheproton-coupledfolatetransporter/hemecarrierprotein1(PCFT/HCP1)andfolatereceptor(FR).Conclusions:Long-termoversupplementationwithf...
-
免试剂离子色谱测定阳离子中的离子抑制器应用研究
...部分2.1仪器及试剂仪器:美国DioneXICS90离子色谱仪,DionexRFC淋洗液发生器,Chromeleon色谱工作站,DS-5型电导检测器,CSRS-I(4mm)抑制器,色谱柱为IonPacCG16保护柱(3mm×50mm)。2.2实验条件淋洗液:采用RFC产生的高纯甲基磺酸溶液(18mmo...
-
小儿反复呼吸道感染发病机制及最新防治
...儿童的外周血T淋巴细胞转化率(LTT)以及活性E花环(Ea·RFC)的变化,实验结果发现复感儿组LTT为(59.74±7.2)%,Ea·RFC为(21.71±4.12)%,明显低于健康对照组,说明复感儿T淋巴细胞数目减少,辅助细胞免疫和体液免疫...
-
Diagnosisofherpessimplexvirus-1keratitisusingGiemsastain,immunofluorescenceassay,andpolymerasechainreactionassayoncornealscrapings
...mark).Theresultswereassessedunderareflectedlightfluorescencemicroscope(BH2-RFC,Olympus)withcubeBhavingfiltercombinationsforexcitationspectrumregionnear490nmforFITCstain.PositivestainingforHSVwasrepresentedbythepresenceofoneormorebasalepithelialcellsexhibitingspecificbrightapplegreenfluorescence.Apre...
-
无创正压通气治疗AECOPD并发Ⅱ型呼吸衰竭的疗效观察
...[4]。 AECOPD表现为高气道阻力、高VD/VT、高功能残气量(RFC)和高呼吸功耗等特点,高气道阻力和内源性呼气末正压(PEEPi)的形成造成呼吸机疲劳,呼吸功耗增加[5]。AECOPD合并Ⅱ型呼吸衰竭使病情复杂,增加了治疗难度和死亡率。...
-
CRPornotCRP?ThatIstheQuestion
...geMedicalSchool,RowlandHillStreet,LondonNW32PF,UnitedKingdom.E-mailm.pepys@rfc.ucl.ac.ukC-reactiveprotein(CRP)andcardiovasculardiseaseisaveryhottopicatpresent.Excitementandinteresthavespilledoverdramaticallyfromthescientificliteratureintothemediaandpopularpressleadingtomuchspeculativecomment.However...
-
Sodium-dependentmethotrexatecarrier-1isexpressedinratkidney:cloningandfunctionalcharacterization
...gorganicaniontransporter(OAT)kidney1/2(OAT-K1/OAT-K2),reducedfolatecarrier(rfc),theABCpumpmrp2,andthesodium-dependentratliverMtxtransporter-1(RL-MTX-1).Sekineetal.(34)andSweetetal.(39)independentlyclonedthefirstmemberofthesodium-independentOATfamily(OAT1)fromratkidney.ItsmRNAistranslatedintoaprotein...
-
虚证
...党参、白术等药,能提高免疫机能,若细胞免疫(E—RFC、LTT)低下时,用之更具意义。益气解毒汤的原方设计,用于各型各期单纯疱疹性角膜炎的治疗,系属辨病论治。临床上,当刺激证状重者,邪盛之时,应以驱邪为主,...
-
Folateandhumanreproduction
...ynthase(A2756G),methioninesynthasereductase(A66G),andreduced-folatecarrier(RFC1;A80G)inadultswithDownsyndromeandfoundthatonlythedistributionofthevariantintheRFCgenewasdifferentfromthatofcontrolsubjects.Becauseofapossibleinfluenceoffolateinadequacyongeneticexpression,theeffectoffolicacidfortification...
-
ThekidneyinvitaminB12andfolatehomeostasis:characterizationofreceptorsfortubularuptakeofvitaminsandcarrierproteins
...rbedmostlikelybyacarrier-mediatedmechanisminvolvingthereducedfolatecarrier(RFC)(86).Thedominantfolateforminserumis5-methyl-tetrahydrofolate(5-MTHF)presenteitherfree,boundtohigh-affinityfolate-bindingprotein(FBP),orlooselyassociatedwithotherserumproteinsincludingalbumin(61,132).Thefractionoffolatebou...
-
Advancedglycationendproductsandthekidney
...pelGB,CartwrightK,FoilesPG,FreedmanBI,RaskinP,RatnerRE,SpiniowitzBS,WhittierFC,andWuerthJPfortheACTIONIInvestigatorGroup.Randomizedtrialofaninhibitorofformationofadvancedglycationendproductsindiabeticnephropathy.AmJNephrol24:3240,2004.BoumaB,Kroon-BatenburgLMJ,WuYP,BrünjesB,PosthumaG,KranenburgO,de...
-
Rosiglitazonepromotesdevelopmentofanoveladipocytepopulationfrombonemarrow–derivedcirculatingprogenitorcells
...onjugatedisotype-matchedcontrolantibodies.CellswereanalyzedonaBeckmanCoulterFC500flowcytometer.PloidyanalysiswasperformedonadipocytessuspendedinPBScontainingsaponin(0.3%finalconcentration),PI(25μg/ml),andEDTA(0.1mM).CellswereanalyzedbyflowcytometrywithsingletdiscriminationtodetectGFP+andPI-stained,...
-
RoleofCaspasesinDeathandSurvivalofthePlaqueMacrophage
...ofDNase,substratesimplicatedincelldeath,includingDFF45andreplicationfactor,RFC140,remainedintactintheseproliferatingTcellsdespitethepresenceofactivecaspases.Caspase-SensitiveKinases:ANewSignalingParadigmSurprisingly,ifcaspasesareconsideredonlyasexecutioners,manykinasesareactivatedbycaspases.Inatleas...