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利用噬菌体肽库确定IL-2Rα表位序列①
...r-Pro(Leu)-Ile的噬菌体克隆中;Ser-Ser-Arg包含在10个序列为Lys-Phe(Cys)-Ser-Ser-Arg-Leu(Lys)的噬菌体克隆中(见表2)。表2 DNA测序所得的氨基酸序列 Tab.2 AminoacidsequencesdeducedfromDNAsequencesNoSequence2Lys-Phe-Ser-Ser-Arg-Leu3Ser-Arg-Arg-Trp-His-Asn5Lys-P...
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北大学者最新文章:泛素化的功能及其意义
...tin-ligaseenzymes).首先,E1利用ATP提供的能量在泛素C端赖氨酸(Lys)残基上的羧基基团与自身的半胱氨酸(Cys)残基上的巯基基团间形成高能硫酯键,从而活化泛素分子.然后,激活的泛素通过硫酯键再被接合到E2的Cys残基上.最终,激活的泛素...
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6-1蛋白质分选的基本原理
...02表1一些典型的分选信号功能信号序列输入细胞核-Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val-输出细胞核-Leu-Ala-Leu-Lys-Leu-Ala-Gly-Leu-Asp-Ile-输入线粒体+H3N-Met-Leu-Ser-Leu-Arg-Gln-Ser-Ile-Arg-Phe-Phe-Lys-Pro-Ala-Thr-Arg-Thr-Leu-Cys-Ser-Ser-Arg-Tyr-Leu-Leu-输入质体+H3N-Met-Val-...
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乙酰乙酸脱羧酶的X射线晶体结构被确定
...,FrankWestheimer提出,在活性点上的一个关键赖氨酸残迹(Lys115)的pKa所发生的很大变化是由于它靠近另一个带电的赖氨酸残迹Lys116。现在,乙酰乙酸脱羧酶的X射线晶体结构已被确定,该结构显示,Lys115的pKa变化不是由于Lys116,...
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第三节 遗传性载脂蛋白代谢异常
...变异体无明显血脂的变化,仅有ApoAⅠMarburg病在107位上的Lys缺失,引起轻度的TG升高。ApoAⅠ的Milano变异体(173Arg→Cys)血浆中HDL有所降低,然而冠心病发病率未见增加,ApoAⅠ和ApoCⅢ基因重排导致的变异是可引起家族性ApoAⅠ和ApoC...
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儿科肾小球疾病时肾小管功能的前瞻性对照观察
...行前瞻性观察。将尿β2-微球蛋白(β2-MG)及尿溶菌酶(LYS)作为近端肾小管功能、24h尿Tamm-Horsfall蛋白(THP)作为远端肾小管功能的评估指标,并与血尿素氮(BUN)、血肌酐(BCr)(肾小球功能)相对照。结果BUN、BCr及尿β2-MG、L...
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高血压定位区域犬尿氨酸酶基因多态性与高血压病相关
...相色谱分析(DHPLC)技术对位于编码区且改变氨基酸编码的Lys412Glu(A/G)多态在456例高血压病患者和430例正常对照者中进行分型和关联研究。结果共检测得KYNU基因的16个SNP,其中启动子区6个,编码区2个(均改变氨基酸编码)。Lys412Glu多...
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MllpartialtandemduplicationinducesaberrantHoxexpressioninvivoviaspecificepigeneticalterations
...dHoxa10inspleen,BM,andblood.AnincreaseinhistoneH3/H4acetylationandhistoneH3lysine4(Lys4)methylationwithintheHoxa7andHoxa9promotersprovidesanepigeneticmechanismbywhichthisoverexpressionoccursinvivoandanetiologicroleforMLLPTDgainoffunctioninthegenesisofAML.IntroductionMixed-lineageleukemia(MLL,alsokno...
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B1KininReceptorDoesNotContributetoVascularToneorTissuePlasminogenActivatorReleaseinthePeripheralCirculationofPatientsWithHeartFailure
...rtrial.Duringweek6ofeachtreatment,patientsreceivedanintrabrachialinfusionofLys-des-Arg9-bradykinin(B1agonist;1to10nmol/min),bradykinin(30to300pmol/min),Lys-[Leu8]-des-Arg9-bradykinin(B1antagonist;1to10nmol/min),andnorepinephrine(60to540pmol/min).Bloodflowandt-PAreleaseweremeasuredusingvenousocclusio...
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丁建平研究组CellResearch揭示调控新机制
...域三维结构的研究,发现其催化活性中心附近严格保守的Lys274残基发生了乙酰化修饰,并通过质谱进行了验证。生物化学和乙酰转移酶活性实验表明Lys274的乙酰化修饰是hMOF以分子内催化的方式进行的,而且这种自身乙酰化能促...
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参考资料
...白酶、胃蛋白酶和嗜热菌蛋白酶。 胰蛋白酶专一断裂Lys,Arg的羧基侧肽键,如果对Lys,Arg,CysH进行化学修饰可改变胰蛋白酶的断裂性质。 (1)赖氨酸的修饰。将Lys用顺丁烯二酸酐或甲基顺丁烯二酸杆修饰,则胰蛋白酶仅使Arg...
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InductionofvascularleakagethroughreleaseofbradykininandanovelkininbycysteineproteinasesfromStaphylococcusaureus
...ependenthypotension.ScpAandSspBtogetherinducedthereleaseofleucyl-methionyl-lysyl-BK,anovelkininwithVLandbloodpressure–loweringactivitiesthatareequivalenttoBK.Collectively,thesedatasuggestthatproductionofBKandleucyl-methionyl-lysyl-BKbystaphopainsisanewmechanismofS.aureusvirulenceandbacterialshock....
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Contributionofintestinalmicrobiallysinetolysinehomeostasisisreducedinminipigsfedawheatglutenbaseddiet
...tges{at}fbn-dummerstorf.de.ABSTRACTBackground:Wepreviouslyreportedmicrobiallysinecontributiontoplasmalysinehomeostasisinhumanswithanadequatelysineintake.Objective:Wesoughttoexplorewhetherthelowlysineintakefromawheatglutenbaseddietisbalancedbyenhancedmicrobiallysinecontributioninapigmodel.Desig...
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12-1核被膜
...原,它在胞质中合成后很快积累在核中。其NLS为:pro-pro-lys-lys-lys-Arg-Lys-val,即使单个氨基酸被替换,亦失去作用。图12-7金标记的核质素穿越核孔 NLS由4-8个氨基酸组成,含有Pro、Lys和Arg。对其连接的蛋白质无特殊要求,并且...
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Heparin-BindingDomainsinVascularBiology
...d.6Amongthereasonsthatmakethistasksodifficultisthatheparinisapolydispersepolysaccharidewithaheterogeneoussaccharidesequencethatbindsalargenumberofproteins.Consequently,thereisawiderangeofpossiblebindingsitesalongtheheparinchainaswellasadiversityofheparin-bindingsitesamongthenumerousproteinsthatbindt...
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Aminoacidsasmodulatorsofendothelium-derivednitricoxide
...dedtotheperfusate.Theadditionof10-4or10-3Mcationicaminoacids(L-ornithine,L-lysine,orL-homoarginine)orneutralaminoacids(L-glutamine,L-leucine,orL-serine)totheperfusatedecreasedNOandincreasedrenalvascularresistance.Perfusionwithanionicaminoacids(L-glutamateorL-aspartate)hadnoeffectoneitherparameter.Th...
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微生物来源的具有促进纤溶作用的低分子化合物的研究进展
...flowmolecularweightcompoundsfromthemicrobialmetabolitesthatenhancingfibrinolysisWUWen-hui,BAOBin.DepartmentofMarinePharmaceuticalBiotechnologyCollegeofFoodScience,ShanghaiFisheriesUniversity,Shanghai200090,China【Abstract】Thepaperintroducesfibrinolyticsystem,structureandactivationofplasminogenw...
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南海海洋所揭示L-别异亮氨酸的生物合成机制
...希夫碱的形式形成PLP-L-Ile复合物;“氨基转移酶”MfnO的Lys206(或DsaD的Lys198)对PLP-L-Ile复合物中l-Ile的a碳位去质子化,形成醌式中间体复合物;异构酶MfnH的Arg107(或DsaE的Arg115)先对醌式中间体复合物的b碳位进行去质子化,形成...
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SLC4base(HCO3
...sport,andthespecificionsthataretransported,SLC4familymemberscanbeconvenientlyseparatedintothreefunctionalgroupswithpotentialtransportmodesforHCO3and/orCO32(Fig.1):1)Na+-independentCl/HCO3exchangersmediatingelectroneutralexchangeofClforbase(HCO3,CO32);2)Na+-HCO3cotransportersmediatingcotransportofNa+...
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清华大学生物化学试题(硕士)1998年-1996年
...哪种氨基酸溶液不使平面偏振光发生偏转(a)Pro(b)Gly(c)Leu(d)Lys()⒉对哺乳动物来说,下列哪种氨基酸是非必须氨基酸(a)Phe(b)Lys(c)Tyr(d)Met()⒊作为典型的催化剂酶具有下面哪种能量效应(a)降低反应的自由能(b)降低反应的活化能(c)...
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刘勇军教授Nature子刊免疫学新文章
...SPRY-PRY结构域与DDX41的DEADc结构域发生了相互作用。DDX41的Lys9和Lys115位点是TRIM21介导的泛素化作用的靶点。这些研究结果表明TRIM21是一种干扰素诱导的E3连接酶,其可诱导DDX41Lys48(K48)关联的泛素化及降解,负调控了对于细胞内dsDNA...
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tuftsin及其抑制物的化学合成和对巨噬细胞抗胆管癌作用的影响
...c方案,起始采用0.0125mmol/L树脂酸(美国ABI公司),按照Thr-Lys-Pro-Arg的序列使肽链从C端逐个向N端延伸。各种氨基酸的保护基团是:各氨基酸的α-氨基为Fmoc保护,其它侧链保护基团为Thy(TrT),Lys(Boc),Arg(PMC)。每步缩合都加入HoBt/Dcc...
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OntheMechanismofActionof9-O-ArylalkyloximeDerivativesof6-O-Mycaminosyltylonolide,aNewClassof16-MemberedMacrolideAntibiotics
...pendentpoly(Phe)synthesis.However,theyseverelyinhibitpoly(A)-dependentpoly(Lys)synthesisandcompetewitherythromycinortylosinforbindingtocommonorpartiallyoverlappingsitesintheribosome.Accordingtofootprintinganalysis,thelactoneringofthesecompoundsseemstooccupytheclassicbindingsiteofmacrolidesthatisloca...
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MastoparanChangestheCellularLocalizationofGq/11andGthroughItsBindingtoGangliosideinLipidRafts
...e,wehavedemonstratedpreviouslythatmastoparansuppressedphosphoinositidehydrolysisinducedbycarbacholinhumanastrocytomacells(FEBSLett206:91–94,1990).Inthepresentstudy,weexaminedwhethermastoparanaffectedphosphoinositidehydrolysisbyinteractingwithlipidraftsinPC-12cells.MastoparaninhibitedUTP-inducedinc...
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RoleofNuclearFactor-BandProteinKinaseCSignalingintheExpressionoftheKininB1ReceptorinHumanVascularSmoothMuscleCells
...A)]allincreasedthedensityofbindingsitesforthetritiatedB1receptoragonist[3H]Lys-des-Arg9-bradykinin(withoutchangeinreceptoraffinity)incell-basedassays.SmallinterferingRNAassaysindicatedthatNF-Bp65isnecessaryfortheeffectiveexpressionofthecellsurfaceB1receptorunderbasalorIL-1,fetalbovineserum(FBS),orPM...
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XPD/ERCC2多态性与晚期结直肠癌对奥沙利铂敏感性关系
【摘要】目的了解XPD/ERCC2Lys751Gln(C→A,35931)多态性与晚期结直肠癌对奥沙利铂为主化疗敏感性的关系。方法70例Ⅳ期结直肠癌病人化疗前取静脉血并提取DNA,以real-timePCR法对XPD/ERCC2基因进行单核苷酸多态性(SNP)分型。对病人行...
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RoleofEctodomainLysinesintheSubunitsoftheHeteromericP2X2/3Receptor
【关键词】ectodomainLysineresiduesneareachendofthereceptorectodomain(inratP2X2Lys69andLys308)havebeenimplicatedinATPbindingtoP2Xreceptors.WerecordedmembranecurrentsfromhumanembryonickidneycellsexpressingP2Xsubunitsandfoundthatlysine-to-alaninesubstitutionsatequivalentpositionsintheP2X3receptor(...
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MolecularDeterminantsoftheAgonistBindingDomainofaP2XReceptorChannel
...dues.Here,weusedtheknownsequenceandsecondarystructuresimilaritiesbetweentheLys180-Lys326ectodomainregionofP2X4andtheclassIIaminoacyl-tRNAsynthetasesasaguidetogenerateathree-dimensionalmodelofthereceptor-bindingsiteandtodesignmutants.Theinterplaybetweenhomologymodelingandsite-directedmutagenesissugge...
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Mitochondrialaldehydedehydrogenase-2(ALDH2)Glu504Lyspolymorphismcontributestothevariationinefficacyofsublingualnitroglycerin
...y,weshowthatthecommonG-to-Apolymorphisminexon12ofALDH2—resultinginaGlu504LysreplacementthatvirtuallyeliminatesALDH2activityinbothheterozygotesandhomozygotes—isassociatedwithalackofefficacyofsublingualGTNinChinesesubjects.Wealsoshowthatthecatalyticefficiency(Vmax/Km)ofGTNmetabolismoftheGlu504prot...
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HeritabilityofClotFormation,Morphology,andLysis
...摘要】Objective—Therelativebalancebetweenclotformationandfibrinolysisisconsideredtoreflectthromboticpotentialfollowingvascularinjury.Theaimsofthepresentstudywereto(1)todeterminethecontributionofgeneticandenvironmentalfactorstovarianceinmeasuresofclotstructure/functionintheLeedsFamilyStudy,a...