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电化学发光免疫分析法测定TSH、FT3、FT4的临床应用
...3 电化学发光免疫分析法(electro-chemiluminescenceimmunoassay,ECLI)是继放射免疫、酶免疫、荧光免疫、化学发光免疫分析法之后的新一代标记免疫测定技术,是电化学发光(ECL)和免疫测定相结合的产物[1]。它包括了电化学和化学发...
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第三节 化学发光免疫测定
... 在电化学发光免疫测定(electrochemluminescenceimmunoassay,ECLI)中应用的标记物为电化学发光反应的底物三联吡啶钌,其衍生物N-羟基琥珀酰胺(NHS)酯可通过化学反应与抗体或不同化学结构的抗原分子结合,制成标记的抗体或抗...
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UniqueMatrixStructureintheRoughEndoplasmicReticulumCisternaeofPseudoachondroplasiaChondrocytes
...timatelyprecipitateschondrocytedeath.28,29Thislossofchondrocytesresultsintheclinicalphenotypeofshortstatureandstructuralabnormalitiesofthebonethatcontributestojointabnormalities.Previously,weevaluatedCOMP,typesIIandIXcollagens,andMATN3usingimmunostainingandelectronmicroscopytodescribeandlocalizethes...
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SuperoxideenhancesNa-K-2Clcotransporteractivityinthethickascendinglimb
...throughtheNa-K-2Clcotransporter.Therefore,wetestedwhetherO2couldalsoincreaseCli.Inisolated,perfusedTHALs,additionofXOandHXtothebathdecreasedSPQfluorescenceby28%(P<0.004;n=5;Fig.2).BecausewepreviouslyshowedthatO2increasesnetJCl,thesedatalikelyindicatethatO2increasesNaClentry.BecauseO2increasedbothNai...
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Double-blind,Placebo-controlledTrialofPirfenidoneinPatientswithIdiopathicPulmonaryFibrosis
...nicalstudyofpirfenidoneinpatientswithadvancedIPF(23),weundertookaprospectiveclinicaltrialtodetermineitseffectonSpO2withexerciseanditscorrelationwithrestingPFTs.ThestudywasabortedinfavorofpirfenidonetreatmentduetoanincreasednumberofacuteexacerbationsofIPFintheplacebogroup.Theresultsofthistrialwerepre...
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DecorinDeficiencyEnhancesProgressiveNephropathyinDiabeticMice
...econcentrationsbyhigh-performanceliquidchromatography.Therewasasignificantdeclineinrenalfunctionby6monthsofdiabetesastheDcnC/CdiabeticgroupsshowedplasmacreatinineconcentrationssignificantlyabovethenormallevelandthediabeticDcn+/+group(P<0.05)(Figure2,BandC).At10monthsofdiabetes,therewasanevengreateri...