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GeneticsforOphthalmologists:theMolecularGeneticBasisofOphthalmicDisorders
...thorbusy,thereadercansitbackintheconfidencethatanextensiveliteraturereviewhasalreadybeendoneallowingmoretimetobespentwiththepatientdiscussingtheimpactofthiswealthofinformation.作者:AmandaChurchill
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转基因鹰嘴豆植物对黑豆蚜的抗性
...转基因鹰嘴豆。该转基因植物表达一种大蒜凝集素基因(asal)。这种大蒜凝集素基因编码甘露糖结合同型二聚体蛋白。凝集素是糖结合蛋白,能介导多个生物过程,如细胞-细胞和宿主-病原物之间的相互作用。部分凝集素的杀虫...
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异丙酚-芬太尼复合用于人工流产的临床观察
....1一般资料选门诊孕6~10周,年龄18~40岁,体重41~74kg,ASAl~Ⅱ级,一般情况良好,术前均无明显心肺疾病史,手术时间3~12min。1.2方法(1)麻醉前常规禁食、禁饮6h,入室后患者取截石位,测血压、脉搏、呼吸,用0.9%生理盐...
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Exaggeratedrelativenasal-temporalasymmetryofmacularcapillarybloodflowinpatientswithclinicallysignificantdiabeticmacularoedema
...MOcomparedwithagematchednon-diabeticsubjects(ANCOVA,p=0.0011)whilerelativenasal-temporalasymmetryofmacularcapillarybloodflowwassignificantlyhigher(p=0.0125).Nasal-temporalasymmetryofmacularcapillarybloodflowwassignificantlyhigherforthepatientswithDMOandcapillaryleakagewithinthescanarea(twotailedttes...
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腺病毒载体疫苗免疫途径的研究(基金项目)
...redividedinto5groups,eachgrouphad10mice.TheAdHBVvaccineswereinoculatedbynasal,subcutaneoustissueineverygrouprespectively.ThespecificantibodywastestedbyELISAin35d,72dandcomparedwiththegroupinjectedwithaluminium-adjuˉvantedvaccines,anddiscussedtherelationshipofinoculativeapproachandthelevelof...
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重建腮腺咬肌筋膜预防Frey综合征
...和错位交叉再生联合,近年来许多学者进行了不懈探索,如Asal等[3]用胸锁乳突肌瓣填塞于术腔,Sinha等[4]报道术中应用无细胞真皮材料(AlloDerm)预防Frey综合征,曲延刚等[5]报道术中采用涤轮薄片作为组织屏障,均取得了一定的效果。...
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CritiqueoftherequirementforvitaminE
...rticularlysuitedfortheintendedresearch.Briefly,onegroupof19subjectswasfedabasaldietcontaining34mgtocopherol/d,anothergroupof9subjectsreceivedthesamedietplusasupplementof15mgRRR--tocopherylacetate/d,andathirdgroupof10subjectswasfedtheregularhospitaldiet.(Theplanwassimilarto3previouslong-termstu...
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基底细胞样乳癌临床病理分析
...xploretheclinicopathologiccharacteristics,diagnosticcriteriaandprognosisofbasal-likebreastcancer(BLBC).MethodsByusingimmunohistochemicaltechnique,121patientswithBLBCwereselected,andtheirER,PR,HER2,AR,EGFRandP-cadherinweredetectedandtheclinicopathologicfeaturesaswellasprognosis-concerningfactorsanaly...
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第四节 肝脏疾病的酶学诊断
...氨酰基转移酶(GGTP),其它有精氨酸代琥珀酸裂解酶(ASAL)、酰苷脱氨酶(ADA)、5’-核苷酸酶、甘露醇脱氢酶等。总的说来这些酶都能比较确切反映肝胆系统的炎症或坏死性病变,在我国对反映肝脏合成功能方面的酶试验重...
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RiskfactorsforagerelatedcataractinaruralpopulationofsouthernIndia:theAravindComprehensiveEyeStudy
...easingage(p0.001).Themostcommonlocationforcorticallensopacitieswasthelowernasalquadrant(n=799,57.2%).Evenamong235eyeswithminimalcorticalchangesbetween0to1LOCSIIIgrade,themostcommonlocationwasthelowernasal(n=130,55.3%),andlowertemporalsegment(n=95,40.4%).Wefoundhypertension(OR1.3,95%CI:1.1to1.6,deff=...
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Alteredpostprandialglucose,insulin,leptin,andghrelininlivercirrhosis:correlationswithenergyintakeandrestingenergyexpenditure
...cirrhosisarenotcompletelyunderstood.Bothpoordietaryintake(3-5)andincreasedbasalenergyexpenditurehavebeenreportedtocontributetoanegativeenergybalanceinpatientswithcirrhosis(1,6-11).Insulinresistanceiscommoninpatientswithcirrhosis(3,7,12)andispossiblyassociatedwithimpairmentofnutritionalstatus(12).Ane...
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Anunfermentedgelcomponentofpsylliumseedhuskpromoteslaxationasalubricantinhumans
...presentsunfermentedPSH,whichcontributestothelaxationpropertiesofPSHbyactingasalubricatingemollienttofacilitatethepropulsionofcoloncontents.SUBJECTSANDMETHODSSubjectsTwenty-oneof33individualswhorespondedtolocaladvertisementswereenrolledinthescreeningphase.Criteriaforexclusionfromthescreeningphasewere...
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Predictorsofbodyfatgaininnonobesegirlswithafamilialpredispositiontoobesity
...eradjustmentforbaselineweight,group,time,ethnicity,andTannerstage,sleepEE,basalEE,24-hEE,andpeakoxygenuptakewerenegativelyassociatedwithFMand%BF(P0.04).Afteradjustmentforthesamevariables,muscleoxidativecapacityandfree-livingtotalEEwerenegativelyandpositivelypredictive,respectively,ofchangesin%BFbetw...
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Acyl-CoenzymeA(CoA):CholesterolAcyltransferaseInhibitioninRatandHumanAorticSmoothMuscleCellsIsNontoxicandRetardsFoamCellFormation
...remightbeadose-dependentorcelltype-specificcytotoxiceffectofACATinhibition.Asalludedto,inatheroscleroticlesionstherearealsoarterialsmoothmusclefoamcells.Intissueculture,SMCscanbeinducedtoformfoamcells,11–13butthepreviouslyreportedcholesterol-loadingmethodshavebeenrelativelyslowandinconvenient.11,1...
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儿童慢性鼻窦炎鼻内镜手术的护理进展
...ion,infection,edemaandallergicreactionbecauseoftheanatomyofthechildren‘snasalcavity.ThenursingcarebecomesmoreandmoreimportantastheconceptofComprehensiveTreatmentwasacceptedandaffectstheresultofthesurgery.Thearticlesummarizestheprogressofthenursingcareaboutendoscopicsinussurgeryforpediatricchronics...
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MerReceptorTyrosineKinaseSignalingParticipatesinPlateletFunction
...NAwasthenusedtoamplifythe3receptors.Astrongexpressionofmerinhumanplateletswasalsodetected(Figure1A).However,axlandrsewerenotdetectedinplatelets,but623-and558-bpbandscorrespondingtotheaxlandmergenes,respectively,weredetectedinbraintissue(Figure1A).Asimilarexpressionpatternwasseeninplateletprecursorce...
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黄疸
...冬近酸氨基移换酶(AST),其他还有氨酸琥珀酸裂解酶(ASAL)、醛缩酶(ALD)、精近酸酶(ARG)和鸟氨酸氨甲基移换酶(ChE),在肝炎时,血内含量下降,②反映胆道病变的酶类,如碱性磷酸酶(ALP)和γ-谷氨酰转肽酶(GGT)...
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ChronicdDAVPinfusioninratsdecreasestheexpressionofP2Y2receptorininnermedullaandP2Y2receptor-mediatedPGE2releasebyIMCD
...ectively.P2Y2-R-mediatedPGE2releasebyfreshlypreparedIMCDwasassayedusingATPSasaligand.ChronicdDAVPinfusionresultedinlow-outputofconcentratedurineandsignificantlyincreasedtheAQP2proteinabundanceinIM.Onthecontrary,dDAVPinfusionat5or20ng/hsignificantlydecreasedP2Y2-Rproteinabundance(40%ofsaline-treatedg...
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TempolreducesoxidativestressandrestoresrenaldopamineD1-likereceptor-Gproteincouplingandfunctioninhyperglycemicrats
...totestthehypothesisthatoxidativestressassociatedwithhyperglycemiaincreasesbasalD1-likereceptorserinephosphorylationviaactivationofthePKC-Gproteinreceptorkinase(GRK)pathway,resultinginlossofD1-likereceptor-Gproteincouplingandfunction.WeobservedthatSTZ-treatedratsexhibitedoxidativestressasevidencedbyi...
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StructuralRequirementsofTransmembraneDomain3forActivationbytheM1MuscarinicReceptorAgonistsAC-42,AC-260584,Clozapine,andN-Desmethylclozapine:
...scarinicagonists(LuandHulme,1999).Theprimaryfeatureofacetylcholinebindingisasaltbridgebelievedtoexistbetweenthecholineheadgroupofacetylcholineandaspartate105(Asp105)inTM3.Thisresiduereactedwiththeaffinitylabelacetylcholinemustard,inwhichtheoniumheadgroupofacetylcholineisreplacedbyahighlyreactiveazir...
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Ouabainmodulationofendothelialcalciumsignalingindescendingvasarecta
...onwithSEA-0400(10-6M)elevated[Ca2+]CYT,supportingaroleforNCXinthesettingofbasal[Ca2+]CYT.SEA-0400abolishedthe[Ca2+]CYTresponsetoouabainimplicatingNCXasamediator.Thetransientpeakphaseof[Ca2+]CYTelevationthatfollowedeitherouabainorreductionof[Na+]owasabolishedby2-aminoethoxydiphenylborate(5x10-5M).Cat...
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CellulardistributionofthepotassiumchannelKCNQ1innormalmousekidney
...cidsecretion.GenomicmicroarrayanalysisofisolatedgastriccellsidentifiedKCNQ1asalikelycandidatefortheK+-effluxchannelassociatedwithH+-K+-ATPaseinthestomach(19).KCNQ1-knockoutmiceexhibithypochlorhydria,hypergastrinemia,gastrichyperplasia,andvacuolationoftheparietalcells,allofwhichsuggestthatKCNQ1isnece...
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CriticalRoleforSphingosineKinase-1inRegulatingSurvivalofNeuroblastomaCellsExposedtoAmyloid-βPeptide
...F-Iprotectiveeffect.SimilarlytoIGF-I,theneuroprotectiveactionofTGF-β1wasalsodependentonSphK1activity;activationofSphK1aswellascellsurvivalwereimpededbyadominant-negativeformofSphK1.Takentogether,theseresultsprovidethefirstillustrationofSphK1roleasacriticalregulatorofdeathandsurvivalofAβ-tr...
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B-Crystallinisanoveloncoproteinthatpredictspoorclinicaloutcomeinbreastcancer
...bstractRecentgeneprofilingstudieshaveidentifiedanewbreastcancersubtype,thebasal-likegroup,whichexpressesgenescharacteristicofbasalepithelialcellsandisassociatedwithpoorclinicaloutcomes.However,thegenesresponsiblefortheaggressivebehaviorobservedinthisgrouparelargelyunknown.Herewereportthatthesmallhea...
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StructuralFeaturesoftheGlutamateBindingSiteinRecombinantNR1/NR2AN-Methyl-D-aspartateReceptorsDeterminedbySite-DirectedMutagenesisandMolecular
...n.Thecurrentresponsetoamaximalagonistconcentrationforallconstructsusedherewasalwayslessthan3e藺ataholdingpotentialofeC40mV.TheopenprobabilityofNMDAreceptorscontainingmutationsineithertheS1orS2bindingsitewasassessedbycoexpressingthesesubunitswithNR1subunitscarryingtheA652Cmutation(Jonesetal.,2002;H....
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KininsPromoteB2ReceptorEndocytosisandDelayConstitutiveB1ReceptorEndocytosis
...lyactive(Leeb-Lundbergetal.,2001).Furthermore,B1Risnotphosphorylatedeitherbasallyorinresponsetoagonist(Blaukatetal.,1999)anddesensitizesonlymarginallyuponfurtherstimulationbytheagonist(Mathisetal.,1996).Ontheotherhand,B2Rissubjecttobothbasalandagonist-promotedphosphorylation(Blaukatetal.,1999,2001)a...