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人间质肺病毒引起的儿童呼吸道感染逐渐增多
...杂志》(JMedVirol2004;75:101-104)上将报告,人间质肺病毒(hMPV)似乎是儿童呼吸道感染一种新出现的病因。米兰大学的普林西皮(NicolaPrincipi)和同事指出,自从2001年发现hMPV以后它的报告越来越多,但几乎还没有儿童期hMPV总负荷...
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保定地区儿童偏肺病毒感染的研究
...呼吸道感染常见病原体检测阴性的标本,进行偏肺病毒(hMPV)基因检测。抽取10份阳性扩增产物进行测序和比较。结果315份标本中共检测到82份阳性扩增产物,阳性率为26.0%。占总检测例数的15.0%。结论保定地区部分患儿的急性呼...
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儿童偏肺病毒感染的探讨
...道感染常见病原体检测阴性的标本,进行人类偏肺病毒(hMPV)基因检测。抽取10份阳性扩增产物进行测序和比较。结果157份标本中共检测到41份阳性扩增产物,阳性率为26.1%。占总检测例数的15.0%。结论保定地区部分患儿的急性呼...
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重庆市“hMPV感染发病机制及疫苗研究”取得重大进展
近日,我市首批杰出青年科学基金项目“hMPV感染发病机制及疫苗研究”通过验收。项目研究取得一系列重大进展:首次阐明N连接糖基化在融合功能和病毒复制中的重要作用,初步明确了hMPV分子流行病学特点;建立了目前国内最...
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人偏肺病毒的分离研究
...见呼吸道病毒病原。上清接种于Vero-E6和LLC-MK2细胞,进行hMPV分离,出现细胞病变者,扩增hMPVN、F基因,F基因扩增产物序列递交GenBank。收集并分析hMPV感染患儿的临床资料。重庆医科大学附属儿童医院P2实验室毛华伟等研究人员研...
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ELISA方法检测儿童中人类偏肺病毒感染
...息-《感染疾病杂志》报道,间接酶联免疫吸附试验利用hMPV中分离得到重组可溶性融合(F)糖蛋白,筛选来自泰国的1380对急性及回复阶段患儿的血清样本。人类偏肺病毒(hMPV)是一种新发现的引起的急性呼吸系统疾病的副粘病毒...
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北京地区人群中人偏肺病毒血清抗体水平的初步调查
...志2005Vol.43No.12P.904-9076(北京)为了通过对人类偏肺病毒(hMPV)特异性IgG抗体检测,初步了解北京地区人群中该病毒感染的状况,研究者以大肠杆菌表达的hMPV核蛋白(N蛋白)为抗原,用Western-blot检测随机选取的116例血清标本中hMPVN蛋...
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双重病毒感染可能引起婴儿的重度细支气管炎
...志》(JInfectDis2005;191:382-386)上报告,人类间质肺病毒(hMPV)和人类呼吸道合胞病毒(hRSV)的双重感染可能引起婴儿的重度细支气管炎。第一作者、利物浦皇家儿童医院的山普(MalcolmG.Semple)告诉路透社记者,“hMPV和hMSV双重感...
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双重病毒感染可能引起婴儿的重度细支气管炎
2005年10月03日JInfectDis2005;191:382-3865人类间质肺病毒(hMPV)和人类呼吸道合胞病毒(hRSV)的双重感染可能引起婴儿的重度细支气管炎。第一作者、利物浦皇家儿童医院的山普(MalcolmG.Semple)告诉路透社记者,“hMPV和hMSV双重感染和...
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DetectionofSevereHumanMetapneumovirusInfectionbyReal-TimePolymeraseChainReactionandHistopathologicalAssessment
...lity,especiallyinimmunocompromisedhosts,buttheimpactofhumanmetapneumovirus(hMPV)inthissettingwaspreviouslyunknown.Methods.Weevaluatedconsecutivebronchoalveolarlavageandbronchialwashfluidsamplesfrom688patients72%wereimmunocompromisedandwerepredominantlylungtransplantrecipientsforhMPVbyuseofquantitati...
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DetectionofHumanMetapneumovirusinClinicalSamplesbyImmunofluorescenceStainingofShellVialCentrifugationCulturesPreparedfromThreeDifferentCell
...TRACTMonoclonalantibodyMAb-8wasevaluatedfordetectionofhumanmetapneumovirus(HMPV)inshellvialcentrifugationcultures(SVCC).DetectionofHMPVwassimilarinA549,HEp-2,andLLC-MK2SVCC,andMAb-8stainingwasoptimalonday2postinoculation.AvailabilityofSVCCforHMPVwillbeofsignificantbenefittoclinicallaboratories.TEXTS...
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RapidDetectionofHumanMetapneumovirusStrainsinNasopharyngealAspiratesandShellVialCulturesbyMonoclonalAntibodies
...diPavia,27100Pavia,ItalyABSTRACTMonoclonalantibodiestohumanmetapneumovirus(hMPV)weredevelopedfordirectfluorescentantibody(DFA)stainingofnasopharyngealaspiratesfrom40infantswithrespiratoryinfections,aswellasforhMPVidentificationinshellvialcultures.Withreferencetoreversetranscription-PCR,DFAstainingsh...
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HumanMetapneumovirusInfectionPlaysanEtiologicRoleinAcuteAsthmaExacerbationsRequiringHospitalizationinAdults
...Medicine,Baltimore,MarylandWedeterminedtheprevalenceofhumanmetapneumovirus(hMPV)infectioninadultswithasthmawhowereprospectivelyenrolledafterhospitalizationforanacuteasthmaexacerbation.Nasalwashspecimenscollectedatadmissionand3monthsafterdischargeweretestedforhMPVbyreal-timereverse-transcriptionpolym...
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RespiratoryViruses:FrequentPrecipitatorsofAsthmaExacerbationsandBeyond
...dadultswithasthma.Williamsetal.reportontheprevalenceofhumanmetapneumovirus(hMPV)infectioninadultshospitalizedforanacuteasthmaexacerbation.Intheirprospectiveobservationalstudyconductedovera4-yearperiod,hMPVwasdetectedinnasalwashspecimensfrom7(6.9%)of101enrolledadultsatthetimeofhospitalizationforanacu...
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HumanCoronavirusNL63InfectioninCanada
...ialviruses(RSVs),adenoviruses,andtherecentlyidentifiedhumanmetapneumovirus(hMPV).However,theetiologicalagentsforalargenumberofrespiratoryinfectionsremainunknown.Anewhumancoronavirus,NL63(HCoV-NL63),hasbeendiscoveredinTheNetherlands[2].Theviruswasidentifiedinclinicalspecimensfrombothinfantsandadultss...
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DetectionofHumanMetapneumovirusRNASequencesinNasopharyngealAspiratesofYoungFrenchChildrenwithAcuteBronchiolitisbyReal-TimeReverseTranscript
...ultedeMedecinedeReims,Champagne-Ardenne,FranceABSTRACTHumanmetapneumovirus(HMPV)wastheuniqueviralpathogendetectedbyareal-timereversetranscriptasePCR(RT-PCR)assayin6(6.4%)of94consecutiveFrenchchildrenhospitalizedforacutebronchiolitisfromSeptember2001toJune2002.Thisviruswasidentifiedasthethirdetiologi...
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DualInfectionofInfantsbyHumanMetapneumovirusandHumanRespiratorySyncytialVirusIsStronglyAssociatedwithSevereBronchiolitis
...ssociationbetweenseverebronchiolitisanddualinfectionbyhumanmetapneumovirus(hMPV)andhumanrespiratorysyncytialvirus(hRSV)wasinvestigatedin2-year-oldinfantswithbronchiolitiswhowereadmittedtothehospitalduringthe20012002winterseason.hMPVinnasopharyngealaspirateand/orcellsandfluidcollectedbynonbronchoscop...
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国内首次发现人类博卡病毒可感染儿童呼吸道
...病毒感染主要病原就有呼吸道合胞病毒(RSV)、人偏肺病毒(hMPV)、冠状病毒、流感病毒A型及腺病毒等,而且还有一部分儿童患急性呼吸道感染虽被认为是由病毒引起,但其确切病原并不清楚。根据国外现有研究资料,博卡病毒可能...
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AnalysisofReplicationKineticsoftheHumanMetapneumovirusinDifferentCellLinesbyReal-TimePCR
...iredeQuebec“andLavalUniversity,Quebec,CanadaABSTRACTHumanmetapneumovirus(hMPV)isassociatedwithacuterespiratorytractdiseaseespeciallyinyoungchildren.Usingaquantitativereal-timeTaqManPCR,weanalyzedthereplicationkineticsofhMPVindifferentcelllines.OurresultsindicatethathMPVreplicatesslightlymoreeffici...
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EpidemiologicalSurveyofHumanMetapneumovirusInfectioninaLargePediatricTertiaryCareCenter
...HpitalSainte-Justine,Montreal,Quebec,CanadaABSTRACTThehumanmetapneumovirus(hMPV)wasrecentlyidentifiedandlinkedtoacuterespiratorytractinfections(ARTI).Toassesstheclinicalimportanceofthisvirusininfantsandchildren,wedevelopedarapidandefficientreversetranscription-PCR-basedscreeningmethodforalargevolume...
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CDC:肺炎无畏疫苗,住院率不减
...的样本。研究结果发现了一些病原体,包括人偏肺病毒(HMPV),呼吸道合胞体病毒(RSV)和一些尚未有疫苗针对的病毒。这就表明,我们需要更先进的技术去抵御导致肺炎的病原体,以及推广现有的疫苗。Jain说:“导致老年人...
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ComparativeEvaluationofReal-TimePCRAssaysforDetectionoftheHumanMetapneumovirus
...ctionMaterialsandMethodsResultsDiscussionReferencesThehumanmetapneumovirus(hMPV)isanewmemberoftheParamyxoviridaefamilyassociatedwithacuterespiratorytractinfectionsinhumans.Theobjectiveofthisstudywastocomparethesensitivityofreal-timeRT-PCRassaysperformedinaLightCyclerinstrumentanddesignedtoamplifythe...
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HumanCoronavirusNL-63InfectionsinChildren:a1-YearStudy
...ARIresultinginhospitalization(9,13).Morerecently,humanmetapneumovirusvirus(hMPV)hasalsobeenidentifiedasanimportantrespiratorypathogeninchildren(14).In2004,anewhumancoronavirus(HCoV),NL63(HCoV-NL63),wasidentifiedinclinicalspecimensfrombothinfantsandadultswithARIinTheNetherlands(15).Theviruswasalsoide...
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DetectionofHumanMetapneumovirusAntigensinNasopharyngealSecretionsbyanImmunofluorescent-AntibodyTest
...iversityGraduateSchoolofMedicine,Sapporo,JapanABSTRACTHumanmetapneumovirus(hMPV)isarecentlydiscoveredpathogenassociatedwithrespiratorytractinfections,primarilyinyoungchildren,immunocompromisedindividuals,andelderlyindividuals.Reversetranscription-PCR(RT-PCR)hasbeenreportedtobeamoresensitivemethodfor...
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为何安吉丽娜·朱莉测基因要三千美元?
...如HPV的某个亚型序列被Digene专利保护;呼吸道黏液病毒(hMPV)序列被欧洲某公司申请的专利保护起来。”韩健说。 而对于麦利亚德基因公司而言,无论专利案胜负如何,它都不得不思考如何在竞争中获得发展。2015年,该公司...
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EvidenceofaNovelHumanCoronavirusThatIsAssociatedwithRespiratoryTractDiseaseinInfantsandYoungChildren
...atoryandhospitalizedpatients,werescreenedforpresenceofhumanmetapneumovirus(hMPV)byuseofanRT-PCRbasedapproachdescribedelsewhere[12,13]andweresubmittedtotheClinicalVirologyLaboratory,YaleNewHavenHospital,fordiagnostictesting.Specimenswereobtainedfrom1January2002to14February2003.Childrencouldbecountedm...