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联合HCV抗原抗体免疫分析方法早期诊断HCV
...行该项研究。为评价敏感性,新方法采用了76个PCR-阳性和anti-HCV阴性血清,7份天然或商业用血清转换平板包括了17份RNA-阳性和anti-HCV阴性血清及31份anti-HCV阳性血清,20份anti-HCV弱阳性血清,80份病毒感染血清及anti-HCV-阳性血清,10...
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丙型肝炎病毒感染的血清学检测
...epatitisCvirusinfectionLiShoubang,WuYanping,HuGuangwei,etal.JilinProvincialAntiepidemicStation,Changchun130021 Abstract Wetestedanti-HCV,anti-HCVIgMandHCV-RNAinserumsamplesfromgeneraladults,blooddonors,acutehepatitispatientsandlivercirrhosispatientsbythemethodsofELISAandRT-PCRsoastostudythere...
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某农村人群甲、乙、丙、戊和庚型肝炎病毒感染的现况研究
...ctionsinaruralpopulation.Methods Enzymeimmunoassayswereusedfordetectionofanti-HAV,HBsAg,anti-HBs,anti-HCV,anti-HEVandanti-HGV.Anti-HGVpositiveserawerefurthertestedforHGVRNAusingareversetranscriptionnestedPCR.Results Theprevalenceratesofanti-HAV,HBsAg,anti-HCV,anti-HEV,anti-HGVinthestudiedpopulat...
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岳阳市吸毒人群中艾滋病、丙肝、乙肝、梅毒的流行病学调查
...对岳阳市强制戒毒所吸毒人员进行问卷调查,并采血检测Anti-HIV、Anti-HCV、HBsAg和梅毒血清学试验。结果277名吸毒人员中HIV感染率4.69%,HCV感染率81.95%,HBV感染率14.08%,梅毒感染率2.53%,13名HIV感染者均合并感染HCV,静脉吸毒者中HCV...
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HCV感染和肾移植
...敏感。 2HCV的检测方法 HCV的检测方法主要有:①anti-HCV的检测;②应用PCR(polymerasechainreaction)检测HCV-RNA;③HCV基因型的检测等;④另外,临床诊治过程中常辅以肝功能的血清学指标及肝穿结果判明HCV感染的进展程度。因...
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输血前感染性指标检测的结果分析
...关感染性检查项目设置的重要性。方法:ELISA法检测HBsAg,Anti-HCV,Anti-TP,Anti-HIV1/2;用速率法检测ALT。结果:HBsAg阳性505例,占31%,Anti-HCV阳性71例,占4.36%,Anti-TP阳性76例,占4.66%,Anti-HIV1/2阳性65例,占4%,ALT异常457例,占28.04%。结论:输...
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西安市1995~2004年十年血液质量监测分析
...血站,每年随机整群抽检1500人份血样,用ELISA进行HBsAg和Anti-HCV感染指标控制监测。结果从1995~2004年抽检15000人份血样中,检出HBsAg阳性者1人份,检出率为0.0067%;Anti-HCV阳性者5人份,检出率为0.033%;病毒总感染率为0.04%。其中:1...
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丙型肝炎病毒非结构区抗原制备及其相应抗体的检测
...个新的手段和依据。PreparationofhepatitisCvirusnonstructuralregionantigensanddetectionoftheirrelativeantibodiesPENGXiangxin (DepartmentofGeneralInternalMedicine,China-JapanFriendshipHospital,Beijing,100029,China) WANGQinhuan 【Abstract】 Objective Toinvestigatetherel...
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新疆艾滋病高发区献血人群HIV、HCV和HBV核酸检测的探讨
...IV-RNA阳性样本,其中包括3份ELISA漏检HBV(HBsAg阴性)和1份HCV(Anti-HCV阴性)窗口期(核酸阳性)标本,窗口期标本在新疆地区健康献血者中的发生率HBV为0.052%(3/5751),HCV为0.017%(1/5751),HIV为0.034%(2/5751)。结论与HBV/HCV/HIV核酸检测(PCR-荧光法)...
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新一代丙肝抗体检测试剂Elecsys特异性与灵敏度俱佳
...试剂的多中心应用评估研究。结果表明,罗氏诊断Elecsysanti-HCVII检测试剂表现出更突出的检测灵敏度和特异性,同时检测只需18分钟,是临床进行丙肝抗体筛查的理想选择。HCV抗体检测的发展史HCV基因结构HCV最早发现于1989年,它...
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代谢综合征(MS)与HBV/HCV感染
...能性较HbsAg阴性患者为低(校正后比率比:0.84(0.76-0.93))。Anti-HCV阳性也与HDL值较低(男性<40mg/dl,女性<50mg/dl)呈正相关(校正后比率比:1.61(1.37-1.88))。高甘油三酯与anti-HCV阳性呈负相关(校正后比率比:0.63(0.55-0.71)。以上数据表明:MS...
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第二节 传染病科门诊记录
...ryexaminationUrinebilirubin+,urobilinogen1:20+ Bloodforlivenfunctions,anti-HAVIgM,HBsAg,anti-HBs,anti-HBc, anti-HBcIgM,HBeAg,anti-HBe,HBVDNA,DNAP,anti-HCV RpTabVitBcono40S.no.2tid TabGlucurolactonsomg×40S.100mgtidImpression:ChonicpersistenthepatitisB徐耀 复诊记录 ...
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陕西人群TTV病毒的基因序列特征
...。均静脉抽取血液5ml,分离血清,-20℃保存。检测HBsAg、Anti-HBs、HBeAg、Anti-HBe、Anti-HBc、Anit-HCV、TTV-DNA、TTV基因测序。1.2材料和方法1.2.1仪器全自动酶联分析仪(美国)、PCR分析仪(美国)、紫外分光透射仪(上海)、自动凝胶成...
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Nature:终结HCV后,HBV治愈又现曙光?
...。此外,birinapant的应用可以促进HBsAg(乙肝表面抗原)和anti‑HBs(乙肝表面抗体)的早期血清转化(治疗开始后7周),而这正是慢性感染患者的初步治疗目标。考虑到birinapant会促进HBV感染的肝细胞凋亡,HBsAg的转阴就很容易...
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吸毒人群丙型和乙型肝炎病毒感染的血清流行病学研究
...ethods Serumsamplesfromdrugusersandnormalcontrolpeoplewerecollected.SerumantibodiesagainsthepatitisCvirus(HCV)andhepatitisBvirus(HBV)weredetectedbyELISA.Results Theresultsshowedthathepatitisprevalenceissignificantlyhigheramongthedrugusersthanthatinnormalcontrolgroup(forHCV67.49%to3.75%,forHBV90....
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献血者血清抗-HCV阳性与ALT活性的关系
...HCV;丙型肝炎病毒核酸;丙氨酸氨基转移酶Therelationshipbetweenanti-HCVpositiveandtheactivityofALTinserumofblooddonors. ZHONGTu-quan,ZHANGXiao-yun. (ZhanjiangMunicipalCentralBloodStation,Zhanjiang524002,Guangdong,P.R.China)Abstract:ObjectiveToinvestigatetherelations...
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献血员单纯ALT升高59例HBV、HCV标志物及ALT追踪观察
...ooddonorswithpureˉlyALTincreasingatthefirsttestwerefollow-upfortheHBsAg,anti-HBS,anti-HBC,ALT.ResultsNoHBVorHCVmarkerturnedintopositiveinthis59casesduringfollow-up.ALTdecreasedin46cases,with37casesdeˉcreaseddownbelownormallevel.ConclusionMerelyALTincreasingcannotberegardedasthemonitormarke...
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NovelAssayUsingTotalHepatitisCVirus(HCV)CoreAntigenquantificationforDiagnosisofHCVInfectioninDialysisPatients
...erologicalassaysandnucleicacidamplificationtechnology(NAT)forassessingserumanti-HCVantibodyandHCVviremia,respectively.However,currentNATtechniquesareexpensiveandlabor-intensiveandoftenlackstandardization.AnassayprototypedesignedtodetectandquantifytotalHCVcoreantigen(totalHCVcoreAg)proteininserumandp...
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蚌埠市大学生无偿献血5项感染指标检测结果分析
...derstandthestatusoffiveunpaidblooddonationinfectionindices(ALT,HBsAg,Anti-HCV,Anti-HIV,TP)forcol-legestudents,andtoprovideevidenceforthedevelopmentofunpaidblooddonation.MethodsAlltheunpaiddonationpersonnelweredividedintocollegestudentsgroupandordinarycrowdgroup,thenfiveinfectionindic...
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1638例孕妇产前肝炎病毒及梅毒感染分析
...ntouandanalyzethetransmissionrouteandriskfactors.MethodsThesixmarkersofHBV,anti-HCVweredetectedbyusingenzyme-linkedimmunosorbentassay(ELISA);HBV-DNAandHCV-RNAweredeterminedbyusingFQ-PCR.SyphiliswasdetectedbyusingRPR.ResultsThere189outofthe1638pregnantwomenwerepositiveforHBsAg(11.5%),eightof30infants...
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UsefulnessoftheHepatitisCVirusCoreAntigenAssayforScreeningofaPopulationUndergoingRoutineMedicalCheckup
UniteVirusetPseudovirus:MorphogeneseetAntigeniciteLEA3865,UniversiteFranoisRabelais,ToursInstitutinterRegionalpourlaSante,LaRiche,FranceABSTRACTWestudiedtheusefulnessoftherecentlydesignedTrak-CassayforthedetectionandquantificationofthehepatitisCvirus(HCV)coreantigen(Ag)forthescreeningofHCVinfectioni...
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丙型肝炎病毒感染的献血者多项指标的动态变化*──26例12年追踪研究
...ubjectsperiodically,observethedynamicchangesofserum(plasma)HCV-RNA,anti-HCVandALT.Andtoperformliverbiopsies,physicalexaminationandothers.ResultsIn12years,morethan400serialsampleswerecollectedandanalyzedfrom26blooddonorsinfectedwithHCVandliverbiopsywasperformedin10voluntarysubjects.Accord...
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医务人员肝炎病毒感染状况分析
...alprofessionals.Methods140medicalprofessionalswerestudiedontheirserumHBsAg,anti-HBs,anti-HCVusingEIA,andHEV-IgG,HGV-IgGwithELISA.ResultsTheinfectionratesofHBsAg,HCV,HEVandHGVofmedicalprofessionalswere7.8%,1.4%,7.1%and7.1%respectivelywithanoverallinfectionrateof23.5%.Theinfectionratesfortheclinicalgr...
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SimultaneousDetectionofHepatitisCVirus(HCV)CoreAntigenandAnti-HCVAntibodiesImprovestheEarlyDetectionofHCVInfection
...meimmunoassaydevelopedforthesimultaneousdetectionofhepatitisCvirus(HCV)coreantigen(Ag)andanti-HCVantibodies(anti-HCVAb)(MonolisaHCVAg/AbULTRA;Bio-Rad)couldimprovetheearlydetectionofHCVinfection,wecompareditssensitivitytothatofanti-HCV,HCVcoreAg,andHCVRNAassays.Thepopulationsstudiedincluded12blooddon...
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用重组结构区抗原检测抗丙型肝炎病毒IgM抗体方法的建立
...较高的敏感度和特异性。 Establishmentofamethodfordetectinganti-HCVIgMusingrecombinatedstructuralregionantigensWangJian,MinFuyuan,ZhangShumin,etal.BeijingYouanHospital100054 Abstract PresenceofIgMantibodytoviralstructuraleproteincanbetakenasthemarkerofitsrdplication,sothedetecti...
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复检试管标本与复检血辫标本检测结果的差异
...实际工作中仍可检测出输血相关传染病标志物如:HBsAg,Anti-HCV等。由于对献血者的血液检测标本缺乏严格统一的规定,复检试管标本留样五花八门,有使用EDTA-NA2抗凝血液标本的,也有使用枸橼酸盐抗凝血液标本的,也有使用肝...
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丙肝病毒核心抗原与丙肝病毒抗体检测的比较研究
...码]A [文章编号]1004-8685(2007)04-0624–02ComparisonandstudyoncoreantigensandantibodiesresponsetohepatitisCvirusSuoFeng2shuang,LiPing2yan,KangXi2xiong(LaboratoryDiagnosisCenter,BeijingTiantanHospital,Beijing100050,China)[Abstract] Objection:Toinvestigatethesignificanceofdetectionofcoreantig...
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庚型肝炎病毒NS5区部分基因的表达及其在EIA中的初步应用
...Objective TocloneandexpressHGVNS5gene,anEIAmethodwasestablishedtodetectanti-HGVNS5antibody.Methods PartialNS5geneofHGVwasobtainedfromahealthyplasmadonorbyRT-PCR,theninsertedintopRSETAvector,andidentifiedbyrestrictionenzymedigestionandDNAsequencing.ThegenewasclonedintoexpressionvectorpPROEX-1.E.c...
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丙型肝炎病毒感染的献血者15年追踪观察──27例多项HCV感染指标的动态变化研究
...ctswerecollectedperiodically,andmulti-markersincludingserum(plasma)HCV-RNA,anti-HCV,ALTandthrombinogentimeweredetected.Liverbiopsieswereperformedon9volunteereddonorsafter15years‘infection.GeneralphysicalexaminationandB-ultrasoundsonliverandspleenwerealsoperformedonallsubjects.ResultsIn15years,more...
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广州市荔湾区重点、高危人群HIV及HCV感染调查
...tandhighriskpopulationinLiwanarea.MethodsTheepidemiologicalsurveywasmadeandantibodylevelsofof3873samplestoHIVandHCVweredeterminedbyELISA.ResultsTherewere10samplespositiveforHIVAND191positiveforHCVotally.ThepositiveratesofHIVintargetandriskpopulationswere0.83%and0,whilethpositiveratesofHCVindrugusers...