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利用噬菌体肽库确定IL-2Rα表位序列①
...定。结果:选择出31个与5G1结合较强的克隆。获得Ser-Ser-Phe和Ser-Ser-Arg两种保守序列。生物学活性鉴定表明:含Ser-Ser-Arg序列克隆较含Ser-Ser-Phe被抑制效果好。结论:Ser-Ser-Arg是IL-2Rα表位的关键序列。以此表位序列合成的小分子肽...
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GhrelinReceptorInverseAgonists:IdentificationofanActivePeptideCoreandItsInteractionEpitopesontheReceptor
【关键词】Identification[D-Arg1,D-Phe5,D-Trp7,9,Leu11]SubstancePfunctionsasalow-potencyantagonistbutahigh-potencyfullinverseagonistontheghrelinreceptor.Throughasystematicdeletionandsubstitutionanalysisofthispeptide,theC-terminalcarboxyamidatedpentapeptidewFwLXwasidentifiedasthecorestructure,whi...
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DifferentialSpatialApproximationbetweenCholecystokininResidue30andReceptorResiduesinActiveandInactiveConformations
...nitylabelingprobes,replacingthenaturallyoccurringtryptophanwithp-benzoyl-L-phenylalanine(Bpa)orp-nitro-phenylalanine(NO2-Phe).TheBpaprobewasshowntorepresentanantagonist,whereastheNO2-Pheprobestimulatedintracellularcalciumasafullyefficaciousagonist(EC50=81±15nM).Bothligandsboundtothereceptorspecific...
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DifferentialModulationofGlutamatergicTransmissionby3,5-Dibromo-L-phenylalanine
...schizophrenia.Here,wedescribeahalogenatedderivativeofthearomaticaminoacidL-phenylalaninethat1)activatesNMDAreceptors,2)depressespresynapticglutamaterelease,and3)blocksAMPA/kainatereceptors.Theexperimentswereconductedinratcerebrocorticalculturedneuronsbyusingthepatch-clamptechnique.3,5-Dibromo-L-phen...
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STK15基因单核苷酸多态与大肠癌风险
...31日中华肿瘤杂志2006Vol.28No.1P.43-467(北京)为了探讨STK15Phe31Ile基因单核苷酸多态与大肠癌风险的关系。研究者以聚合酶链反应(PCR)和限制性片段长度多态性(RFLP)分析方法,检测了283例大肠癌患者和283例配对的正常对照者STK15Phe31Il...
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30.2遗传病的营养治疗
... (1)苯丙酮尿症与其他苯丙氨酸血症苯丙酮悄症(phenylketonuria,PKU)是遗传性氨基酸代谢异常中研究最多的疾病。属于常染色体隐性遗传病。病者血中苯丙氨酸显著升高。根据苯丙氨酸血症的严重程度及酶缺乏的程度分为8型...
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FunctionalGeneticDiversityintheHigh-AffinityCarnitineTransporterOCTN2(SLC22A5)
...】FunctionalC.WefoundeightaminoacidsequencevariantsofOCTN2,ofwhichthree(Phe17Leu,Leu144Phe,andPro549Ser)werepolymorphicinatleastoneethnicgroup.Whenassayedforfunctionalactivitybyexpressioninhumanembryonickidney293cells,usingasprobesboththeendogenoussubstrate(L-carnitine)andtheorganiccationtetraethyl...
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铁岭市新生儿疾病筛查结果分析
...甲状腺功能减退症4例,发病率为1/3849,筛出苯丙氨酸(Phe)增高患儿6例,其中经典型PKU患儿5例,高苯丙胺酸血症1例,PKU发病率为1/3079。结论新生儿疾病筛查对提高我国人口素质有重大意义,应多环节协作,做好该项工作。 ...
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6-1蛋白质分选的基本原理
...la-Gly-Leu-Asp-Ile-输入线粒体+H3N-Met-Leu-Ser-Leu-Arg-Gln-Ser-Ile-Arg-Phe-Phe-Lys-Pro-Ala-Thr-Arg-Thr-Leu-Cys-Ser-Ser-Arg-Tyr-Leu-Leu-输入质体+H3N-Met-Val-Ala-Met-Ala-Met-Ala-Ser-Leu-Gln-Ser-Ser-Met-Ser-Ser-Leu-Ser-Leu-Ser-Ser-Asn-Ser-Phe-Leu-Gly-Gln-Pro-Leu-Ser-Pro-Ile-Thr-Leu-Ser-Pro-Phe-...
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IdentificationofLeu276oftheS1P1ReceptorandPhe263oftheS1P3ReceptorinInteractionwithReceptorSpecificAgonistsbyMolecularModeling,Site-Direc
...-raycrystalstructureofbovinerhodopsin.ThemodelspredictthatS1P1Leu276andS1P3Phe263contributetotheS1P1/S1P3selectivityofthetwoS1P1-selectiveagonists.Theseresiduesweresubjectedtosite-directedmutagenesis.Thewild-typeandmutantS1PreceptorswereexpressedinChinesehamsterovarycellsandexaminedfortheirabilities...
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四氢生物蝶呤负荷试验诊断四氢生物蝶呤反应性苯丙氨酸羟化酶缺乏症的临床研究
...分析、红细胞二氢蝶啶还原酶测定。对其中血苯丙氨酸(Phe)浓度<600μmol/L者给予口服Phe-BH4联合负荷试验,对部分BH4反应性PAH缺乏症患儿,在普食条件下给予BH4片剂(10~20mg/kg)替代治疗6~7天,观察其疗效。结果(1)在BH4负荷试验中...
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OntheMechanismofActionof9-O-ArylalkyloximeDerivativesof6-O-Mycaminosyltylonolide,aNewClassof16-MemberedMacrolideAntibiotics
...ibitpeptidyltransferase,peptidyl-tRNAtranslocation,orpoly(U)-dependentpoly(Phe)synthesis.However,theyseverelyinhibitpoly(A)-dependentpoly(Lys)synthesisandcompetewitherythromycinortylosinforbindingtocommonorpartiallyoverlappingsitesintheribosome.Accordingtofootprintinganalysis,thelactoneringoftheseco...
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小肠内营养对于成年Crohn病患者蛋白质转换的急性效应
...稳定的同位素对空腹和摄食后检测亮氨酸(Leu)和苯丙氨酸(Phe)动力学。结果可见,为适应小肠内营养,Leu和Phe(反射性蛋白分解)出现(Ra)的内源性率显著减低40%。内脏摄入Leu和Phe的百分比分别为35+/-10%和13+/-12%。在摄食后,Phe对于蛋...
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Thehumanorganicaniontransporter3(OAT3;SLC22A8):geneticvariationandfunctionalgenomics
...lotherswithsignificantlyreducedfunction.Oneofthemorecommonvariants(p.Ile305Phe),foundin3.5%ofAsian-Americans,appearedtohavealteredsubstratespecificity.ThisvariantexhibitedareducedabilitytotransportES,butapreservedabilitytotransportCIM.ThesedatasuggestthatgeneticvariationinOAT3maycontributetovariatio...
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Renalmedullarytissueoxygenationisdependentonbothcorticalandmedullarybloodflow
...owineitherthecortexormedulla.Inanesthetizedrabbits,renalarterialinfusionof[Phe2,Ile3,Orn8]-vasopressinselectivelyreducedmedullaryperfusionandPO2(54±24and50±10%,respectively)butdidnotsignificantlyaffectcorticalperfusionortissueoxygenation.Incontrast,stimulationoftherenalnervesresultedinrenalcortica...
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Restingmetabolicrateandrespiratoryquotient:resultsfromagenome-widescanintheQuebecFamilyStudy
...on-basedandvariancecomponentsbasedgenome-wideautosomalscansonRMRandRQphenotypes,obtainedfromindirectcalorimetry,wereperformedin169familiesascertainedviaanobeseprobandorfromthegeneralpopulation.Results:WefoundevidenceforlinkagetoRMRonchromosomes3q26.1(lod=2.74),1q21.2(2.44),and22q12.3(1.33).QTL...
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苯丙酮尿症的筛查和治疗进展
...型与亚洲其他地区相似,但与欧洲人群有明显的差异[3]。Phe转化为酪氨酸的过程中除需PAH外,还必须有四氢生物蝶呤(tetrahydrobiopterin,BH4)作为辅酶参与。人体内的BH4是由鸟苷三磷酸(guanosinetriphosphate,GTP),经过鸟苷三磷酸环化水...
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6-3内质网
...一些信号肽序列蛋白质信号序列PreproalbuminMet-Lys-Trp-Val-Thr-Phe-Leu-Leu-Leu-Leu-Phe-Ile-Ser-Gly-Ser-Ala-Phe-Ser↓Arg...Pre-IgGlightchainMet-Asp-Met-Arg-Ala-Pro-Ala-Gln-Ile-Phe-Gly-Phe-Leu-Leu-Leu-Leu-Phe-Pro-Gly-Thr-Arg-Cys↓Asp...PrelysozymeMet-Arg-Ser-Leu-Leu-Ile-Leu-Val-Leu-Cys-P...
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中性氨基酸对帕金森病发生干预的实验研究
...前后腹腔注射中性的左旋缬氨酸(L-Val)、左旋苯丙氨酸(L-Phe)、右旋缬氨酸(D-Val)及碱性的左旋赖氨酸(L-Lys),采用免疫组化、Western印迹法观察小鼠脑黑质部位百草枯沉积以及多巴胺能神经元变性和α-突触核蛋白(α-Syn)聚集的改变。...
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High-ThroughputScreeningforSmall-MoleculeActivatorsofNeutrophils:IdentificationofNovelN-FormylPeptideReceptorAgonists
...ogroupsofactivatorsofROSproductioninmurineneutrophils:t-butylbenzeneandthiophene-2-amide-3-carboxylicesterderivatives.Furtherstudiesoftheactivecompoundsrevealed11compoundsthatactivatedROSproductioninhumanneutrophils,andsixofthesecompoundsalsoactivatedintercellularCa2+mobilizationandchemotaxisinhuman...
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Supplementationofsoyproteinwithbranched-chainaminoacidsaltersproteinmetabolisminhealthyelderlyandevenmoreinpatientswithchronicobstructive
...examinedon2testdays.SimultaneouscontinuousintravenousinfusionofL-[ring-2H5]phenylalanine(Phe)andL-[ring-2H2]tyrosinetracerswasdonepostabsorptivelyandat2hofingestionofamaltodextrinsoyormaltodextrinsoy+BCAAproteinmeal(rateofingestion:0.02gprotein·kgbodyweight1·20min1)inacrossover...
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参考资料
...要作用点其它作用点胰蛋白酶胰Arg,Lys 糜蛋白酶胰Tyr,Phe,TrpLeu,Met,His,Asu,Gln弹性蛋白酶胰Leu,Ile,Ala其它等胃蛋白酶胃粘膜Tyr,Phe,Trp,Met,LenAla,Glu,Asp,其它等木瓜蛋白酶Papayplant木瓜植物Arg,Lys,Gly其它等嗜热菌...
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CultivationofNeisseriagonorrhoeaeinLiquidMediaandDeterminationofItsInVitroSusceptibilitiestoQuinolones
...gFB.ColonieswereremovedfromGCIIagarafter24hofincubationat35°Cinamoistatmospherecontaining5%CO2.Thecoloniesweredispersedinsterilesaline,andtheinoculumturbiditywasadjustedtoapproximately1.0atanopticaldensityof520nm(ca.108to109CFU/ml).Atotalof100μlofthissuspensionwastransferredtotesttubescontaining10...
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生物化学考研习题-是非题
...沉淀蛋白质时,溶液的PH值应稍大于蛋白质的等电点。5、Phe-Leu-Ala-Val-Phe-Leu-Lys是一个含有7个肽键的碱性七肽。6、溶液中蛋白质表面上的各个原子之间可形成氢键。7、赖氨酸是必需氨基酸,而粮食中含量甚少,因此,作为食物添...
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邻苯二甲醛柱前衍生反相高效液相色谱法测定水牛角中氨基酸的含量
...酸),Gly(甘氨酸),Arg(精氨酸),Met(蛋氨酸),Val(缬氨酸),Phe(苯丙氨酸),Ile(异亮氨酸),Leu(亮氨酸),Lys(赖氨酸)(均为Sigma产品),实验用水为去离子重蒸水。流动相经过滤、脱气处理。试样溶液用0.45 μm滤膜过滤备用。2.2 色谱分...
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NeutralaminoacidtransportmediatedbyorthologofiminoacidtransporterSIT1/SLC6A20inopossumkidneycells
...ptakes.Na+-dependenttransportrateswereexpressedrelativetotheNa+-dependentL-Phetransportrate.Forsubstrateselectivitymeasurements,5mML-aminoacidsweregivenfor20s.Forcompetitionexperiments,theuptakeof5mML-Phe(20s)wasperformedintheabsence(control)orpresenceof25mMcompetingcoldL-aminoacid,MeAIB,or10mMBCH.F...
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淋球菌对氟喹诺酮耐药性的研究进展
...药菌株均存在GryA基因91位点上丝氨酸(Ser)→苯丙氨酸(Phe)的突变,而所有敏感菌株则无一突变。据此,作者认为:对于氟喹诺酮耐药的淋球菌来说,GryA基因Ser91→Phe突变可能是一个原始突变。而Onodera[10]的研究又发现对氟喹...
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超级恶菌或已出现“最后抗生素防线”对其无效
...发现的MCR-1,结束了多黏菌素的神话。英格兰公共卫生局(PHE)其后在英格兰及威尔斯3个猪场抽取2.4万个样本,其中15个样本发现带有MCR-1的恶菌。科学家忧虑MCR-1会与其他带抗药性的细菌结合,演变成无药可治的“超级细菌”。抗...
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ProteochemometricMappingoftheInteractionofOrganicCompoundswithMelanocortinReceptorSubtypes
...sHindIII,XhoI,andXbaIwerefromPromega(Madison,WI).125I-NDP-MSH(125I-[Nle4,D-Phe7]-MSH)wascustom-synthesizedbyEuro-DiagnosticaAB(Malm,Sweden).ThegenesoftheMC1andMC5Rshadbeenclonedearlierinourlaboratory(Chhajlanietal.,1992,1993).TheMC3andMC4RgenesweregiftsofDr.IraGantz(UniversityofMichiganMedicalSchool...
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肽的溶解性及储存
...疏水性)小的:Cys、Pro、Ala、Thr大的:Val、Ile、Leu、Met、Phe中性氨基酸大的:Trp、Tyr、His极性氨基酸(亲水性)小的:Ser、Gly、Asp、Asn大的:Glu、Gln、Lys、Arg可溶性的问题许多合成的肽粗品,尤其是那些经过几次沉淀和干燥的,...