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酪氨酸酶及其抗体与自身免疫性白癜风
...抗体可以作为白癜风活动性的一个指标。 酪氨酸酶(TYR)是一种75kD含铜酶,来源于胚胎神经峭细胞,是黑素代谢和儿茶酚胺的关键酶。Spritz等[1]证实人TYR是一种铜结合蛋白,有铜A和铜B位点。离子铜特异性能与人TYR结合,一...
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StimulationofLiver-DirectedCholesterolFluxinMicebyNovelN-Acetylgalactosamine-TerminatedGlycolipidsWithHighAffinityfortheAsialoglycoproteinRec
...cvalueinhypercholesterolemia.MethodsandResults-Abifunctionalglycolipid(LCO-Tyr-GalNAc3)withahigh-nanomolaraffinityfortheASGPr(inhibitionconstant2.1±0.2nmol/L)wassynthesizedthatshowedrapidassociationwithlipoproteinsonincubationwithserum.PriorincubationofLCO-Tyr-GalNAc3withradiolabeledlow-densi...
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与CD28有关的协同刺激信号转导
...CD28分子胞浆域含有4个保守的酪氨酸位点(JurkatT细胞中为Tyr170,185,188,197)。其中Tyr170曾倍受关注[4]。Tyr170磷酸化后,可招募含SH-2的信号分子,如PI3-K。后者曾一度成为CD28转导的协同刺激信号途径中的研究热点[5~7,8]...
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FAK在组织损伤中的研究进展及其法医学意义
...酶(focaladhesionkinase,FAK)是非受体蛋白酪氨酸激酶(proteintyrosinekinase,PTK)的一种,主要分布在胞浆中,起着介导细胞黏附、迁移、生存及细胞周期调控等重要作用。FAK与其他信号转导途径的相互作用机制及其在细胞凋亡中的作用...
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外用脱色剂的研究进展
...用机理将外用脱色剂大致分类综述如下:一、酪氨酸酶(TYR)活性抑制剂 TYR是黑素生成的关键酶,对TYR活性的调节,可直接影响黑素的合成。 氢醌:氢醌是最常用的脱色剂,它对TYR活性具有抑制作用。它能有效的治疗各...
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[177Lu-DOTA0,Tyr3]奥曲肽多肽受体靶向放射治疗中骨髓放射量测定
...clearMedicineandMolecularImaging》上的一篇文章指出用[177Lu-DOTA0,Tyr3]奥曲肽进行多肽受体靶向放射治疗(PRRT),骨髓和血液的放射活性相关,同时放射性药物与干细胞的结合没有明显差异,放射性药物有从组织器官和肿瘤向骨髓分布,而...
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生物物理所在金属蛋白理性设计研究方面获得进展
...9日,中科院生物物理所王江云研究组以ProbingtheFunctionoftheTyr-CysCrosslinkinMetalloenzymesthroughtheGeneticIncorporationof3-Methylthiotyrosine为题,在AngewandteChemieInternationalEdition上发表了最新研究成果。该研究通过扩展基因密码子,实现了在活细胞...
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Effectoftyrosinekinaseblockadeonnorepinephrine-inducedcytosoliccalciumresponseinratafferentarterioles
【摘要】Weusedgenistein(Gen)andtyrphostin23(Tyr-23)toevaluatetheimportanceoftyrosinephosphorylationinnorepinephrine(NE)-inducedchangesinintracellularfreecalciumconcentration([Ca2+]i)inratafferentarterioles.[Ca2+]iwasmeasuredinmicrodissectedarteriolesusingratiometricphotometryoffura2fluorescence....
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StructuralBasisforEpibatidineSelectivityatDesensitizedNicotinicReceptors
...inthe104eC117/106eC119regionaredeterminantsofepibatidineselectivity:Lys104/Tyr106,Ser111/Tyr113,andTyr117/Tyr119.Inthe-subunit,simultaneousmutationoftheseresiduestotheirequivalentproduceshighaffinity,-likeepibatidinebinding.However,convertingtoaffinityrequiresreplacementofthe104eC117segmentwithseque...
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Jak/STAT信号转导途径研究新进展
...胞中存在的非跨膜型蛋白酪氨酸激酶(nontransmembraneproteintyrosinekinase,NM-PTKs)可以介导细胞因子与其受体结合后的信号蛋白分子级联活化反应。Jak蛋白酪氨酸激酶(Janusproteintyrosinekinase)即是近几年来鉴定出来的在细胞因子信号...
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SuppressionofthePhosphorylationofReceptorTyrosinePhosphatase-ontheSrc-IndependentSiteTyrosine789byReactiveOxygenSpecies
【关键词】SuppressionOxidationofreceptorproteintyrosinephosphatase-(RPTP)isemergingasanimportantyetpoorlycharacterizedregulatorymechanismforRPTPsignalingincells.RPTPhasbeenshowntobereversiblyoxidizedandinhibitedbyreactiveoxygenspecies.However,itisnotknownwhetheroxidativestresscouldregulatetheph...
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MolecularInteractionsofCCR5withMajorClassesofSmall-MoleculeAnti-HIVCCR5Antagonists
...singmutationdataarefurtherrefinedandvalidatedusingavailablestructure-activityrelationshipinformation.Thesubpocketsarefullycharacterizedforshapeandelectrostaticnature,andarefinedpharmacophoremodelforleadidentificationcanbegeneratedwithease.ThisfullymappedbindingpocketforCCR5hasbeeneffectivelyusedasas...
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HumanChorionicGonadotropinModulatesProstateCancerCellSurvivalafterIrradiationorHMGCoAReductaseInhibitorTreatment
...JNK1/2,andp38MAPK)antibodies,phospho-/total-AKT(S473),phospho-/total-ERBB1(Tyr1068,Tyr1173,Tyr845,andTyr992),andanti-PARP1antibodieswerepurchasedfromCellSignalingTechnologyInc.(Danvers,MA).Theanti-PARP1ADP-ribosylationspecific10HmonoclonalantibodywaspurchasedfromAxxoraLifeSciences,Inc.(SanDiego,CA)....
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AminoAcidsCriticalforSubstrateAffinityofRatOrganicCationTransporter1LinetheSubstrateBindingRegioninaModelDerivedfromtheTertiaryStructu
.../or1-methyl-4-phenylpyridinium(MPP)weredecreased.AfterreplacementofTrp218bytyrosine(W218Y)andTyr222byleucine(Y222L),theKmvaluesforbothTEAandMPPweredecreased.InmutantsY222FandT226A,onlytheKmvaluesforTEAandMPPweredecreased,respectively.ThedatasuggestthataminoacidsTrp218andTyr222participateinthebinding...
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6-1-3多瘤病毒科
...;中T抗原定位于细胞质膜,也是一种膜结合蛋白,其中在Tyr-250、Tyr-315和Tyr-322处存在3个酪氨酸部位,体外均可被酪氨酸特异性激酶系统磷酸化,目前一般认为Tyr-315本身的磷酸化是中T抗原引起细胞转化表型形成的关键过程。小T基...
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ProbingtheExistenceofGProtein-CoupledReceptorDimersbyPositiveandNegativeLigand-DependentCooperativeBinding
...rLifeandAnalyticalSciences(Courtaboeuf,France)Products.4-OH-Phenylacetyl-D-Tyr(Me)2,Phe3,Gln4,Asn5,Arg6,Pro7,Arg8-NH92,(HO-LVA)(Barberisetal.,1995),d(CH2)5-[Tyr(Me)2,Thr4,Orn8,Tyr-NH92]vasotocin(OTA)(Elandsetal.,1988b),d(CH2)5[D-Thi2,Thr4,Orn8,Eda9Tyr10]vasotocin(Manningetal.,2001),and[Phe2,Orn8]vas...
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MastoparanChangestheCellularLocalizationofGq/11andGthroughItsBindingtoGangliosideinLipidRafts
...fmastoparanonphosphoinositidehydrolysis.Furthermore,fluorescenceintensityoftyrosineresidueof[Tyr3]mastoparanwaspotentiatedbygangliosidemixtures,suggestingthedirectbindingofmastoparantogangliosides.MastoparancausedcytotoxicityofPC-12cellsinaconcentration-dependentmanner,determinedbyLDHrelease.Themast...
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参考资料
...裂解Try处的肽键,五十年代研究较多。但由于它也能断裂TyrHis肽键,因此应用不广。 2)酶解法 酶水解法较化学法具有更多的优越性,使用也更广泛。因其具有较高专一性,而且水解产率较高,所以可以选择各种不同专...
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银屑病中T细胞活化的研究进展
...子内流,蛋白激酶C(PKC)的活化、蛋白酪氨酸磷酸化(p-tyr)是调控T细胞激活的中心步骤。Ockenfels等[21]研究了多种治疗或可激发银屑病的药物对银屑病T细胞p-tyr的影响作用,结果发现:与正常对照一样,银屑病T细胞在经PHA刺激...
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高血糖与心血管疾病关系再认识
...含量(%)多数肥胖患者存在瘦素抵抗瘦素信号转导LRbJAK2Tyr985Tyr1138肥胖细胞质JAK2Tyr985Tyr1138SHP2ERKPOMCAgRP细胞核STAT3LL神经肽基因表达PI3激酶细胞膜SOCS3对抗瘦素和胰岛素的信号瘦素受体胰岛素受体瘦素胰岛素细胞因子抵抗素FFAJAK2SOC...
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TargetingtoC-TerminalMyosinHeavyChainMayExplainMechanotransductionInvolvingFocalAdhesionKinaseinCardiacMyocytes
...Pressureoverloadlastingfrom3to60minuteswasshowntoinduceFakphosphorylationatTyr-397,-576/7,-861,and-925asdetectedbyphosphospecificantibodies.ThiswasparalleledbyincreasesofFak/SrcassociationandSrcactivity(Tyr-418phosphorylation).Yeasttwo-hybridscreeningofanadultratcDNAlibraryrevealedaninteractionbetwe...
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IdentificationandCharacterizationofTwoAminoAcidsCriticalfortheSubstrateInhibitionofHumanDehydroepiandrosteroneSulfotransferase(SULT2A1)
...tion.Basedonthestructuralanalyses,substitutionofthesmallerresiduealanineforTyr-238(Y238A)significantlyincreasestheKivaluefordehydroepiandrosterone(DHEA)andtotallyeliminatessubstrateinhibitionforandrosterone(ADT).Inaddition,Met-137wasproposedtoregulatethebindingorientationsofDHEAandADTinSULT2A1.Compl...
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MicrosomalEpoxideHydrolase,Endotoxin,andLungFunctionDeclineinCottonTextileWorkers
...yearprospectivestudyof265workersexposedtoendotoxinand234controlsubjects.mEHTyr113HisandHis139Argpolymorphismsweregenotypedbythe5‘nucleaseassay,anddatawereanalyzedusingmultivariatelinearregressionmodels,adjustingforimportantcovariates.Overall,theannualdeclinerateofFEV1was29.47mlduringthe20-yearfoll...
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清华大学生物化学试题(硕士)1998年-1996年
...ansylchloride)⑺丝氨酸-甘氨酸-酪氨酸-丙氨酸-亮氨酸(Ser-Gly-Tyr-Ala-Leu)⑻7-脱氢胆固醇(7-Dehydrocholesteol)⑼烟酰胺腺嘌呤二核苷酸(辅酶I,NAD+)(Nicotinamideadeninedinucleotide)⑽鸟苷酸(Ornithine)⑾1,3-二磷酸甘油酸(1,3-Bisphosphoglycerate)⑿磷酸烯醇...
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基因与色素沉着和皮肤癌
...,而黑色素瘤则是由色素沉着引发的一种恶性皮肤肿瘤。TYR基因负责编码皮肤黑色素所需要的酶,他们报告了与TYR基因相关的变异。他们所提及的每种基因都与一种常见但不太致命的皮肤癌——基底皮肤癌有关。在第二项独立的...
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利用噬菌体肽库确定IL-2Rα表位序列①
...得到两种保守序列:Ser-Ser-Phe包含在4个序列为Ser-Ser-Phe-Tyr-Pro(Leu)-Ile的噬菌体克隆中;Ser-Ser-Arg包含在10个序列为Lys-Phe(Cys)-Ser-Ser-Arg-Leu(Lys)的噬菌体克隆中(见表2)。表2 DNA测序所得的氨基酸序列 Tab.2 Aminoacidsequencesdeducedfro...
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AT1receptor-mediatedaccumulationofextracellularangiotensinIIinproximaltubulecells:roleofcytoskeletonmicrotubulesandtyrosinephosphatases
...r-mediatedendocytosis,2)thisprocessisregulatedbycytoskeletonmicrotubule-andtyrosinephosphatase-dependentmechanisms,and3)AT1receptor-mediatedendocytosisofANGIIhasafunctionalrelevancebymodulatingintracellularcAMPsignaling.InculturedPTCs,[125I]Tyr-labeledANGIIandfluoresceinlabeled-ANGIIwereinternalized...
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心肌细胞FAK机械信号转导中肌球蛋白C端重链的作用
...结果。认为如果过度的压力负荷持续达3-60分钟,就可在Tyr-397,-576/7,-861和-925位点诱导FAK的磷酸化,这种作用大小与Fak/Src之间的联系性及Src活力(Tyr-418磷酸化)的增加相平行。一项成年鼠cDNA文库的酵母双杂交技术表明了FAK与-肌球...
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InsightsintotheCholecystokinin2ReceptorBindingSiteandProcessesofActivation
...20(ECL1)andinteractedwiththeimidazolegroupofHis381(H7.39)andthephenylringofTyr385(H7.43).Mutagenesisandpharmacologicalstudiesvalidatedtheseinteractions.ItisnoteworthythatthemutationofPhe120toTrpconferredconstitutiveactivitytotheCCK2R.Molecularmodelingandexperimentalworksidentifiedtheresiduesinvolved...
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二、CD45在信号转导中的调节作用
...分子的505位点氨基酸被磷酸化。p56lck激酶活性的升高是与Tyr505去磷酸化相一致。CD45可使有活性激酶中主要自身磷酸化Tyr395发生去磷酸化,从而引起激酶活性的抑制。 2.MAP-2K的去磷酸化 T细胞活化中,CD45另一种可能催化的底...