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HPLC法检测血清中伏立康唑含量的研究
...olecontentinhumanserumsamples.MethodsEliterC18column(200mm×4.6mm,5μm)wasusedwiththemobilephaseofwater-acetonitrile-aceticacid-triethylamine(64:36:0.1:0.05).Theflowratewas1.2ml.min-1andthewave-lengthwas258nm.ResultsThecalibrationcurvewaslinearintherangeof0.14-13.96μg/ml(r=0.998...
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双试剂苦味酸法测定血清肌酐的改进
...口。仪器:BECKMAN-CX5型全自动生化分析仪。1.2方法(1)20μl血清(TBIL:408μmol/L)与200μl试剂R1(300mmol/L氢氧化钠)混合后,连续检测该混合物分别在520nm和620nm处吸光度的变化。结果发现48s以后520nm吸光度下降缓慢或基本不...
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两种诊断标准下2141名学龄前儿童血铅水平分析
...DepartmentofHealthandAmericanCDC.Results:Themeanbloodleadlevelwas73.90μg/L,and224children(10.46%)werefoundwithabloodleadlevel≥100μg/L.Thebloodleadlevelandprevalencerateofbloodloadlevel≥100μg/Lhavenodifferencewithdifferentdistricts.Thehighestlevelofleadwasfoundinchildre...
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新疆吐鲁番盆地新生儿脐带血TSH及孕妇碘营养水平评价
【摘要】30mu/L者278名,占10.9%;新生儿全血滤纸TSH测定分析统计计算正常值范围在4.3~16.4mu/L。测定的2514份孕妇尿碘<25μg/L者112份,占4.5%;<50μg/L者206份,占8.2%;<100μg/L者429份,占17.1%;计算的孕妇尿碘值范围下限为88μg/L,上限为276μg...
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丙泊酚不同效应室靶浓度输注对老年人镇静程度和血液动力学的影响
...老年病人静脉靶控输注丙泊酚,血浆靶浓度的设定从0.5μg/ml开始,当效应室靶浓度上升到血浆靶浓度水平时,将血浆靶浓度调高0.5μg/ml,使丙泊酚血浆靶浓度阶梯式上升,直到效应室靶浓度达到3.5μg/ml。记录每个效应室靶浓...
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反相高效液相色谱法测定头孢米诺的血药浓度
...MethodsChromatographicconditionsincludedDiamonsilC18(250mm×4.6mm,5μm),ammoniumacetatebuffer(pH4.5):methanol(85:15V/V)asmobilephase,paracetamolasinternalstandard.Thedetectivewavelengthwas273nmandtheflowratewas1.0ml/min.ResultsThecalibrationcurvewaslinearintheconcentrationsrangi...
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梅毒螺旋体重复蛋白K的克隆表达
...-guo,XIONGli-kuan,WANGXiao-hong,etal. (Departmentoflaboratory,ShenzhenMunicipalFuyongHospital,Shenzhen518103,Guangdong,P.R.China) Abstract:ObjectiveTocloneandinvitroexpressTprKandprovideagoodfoundationforpreparingprophylacticvaccine.MethodsPCRandgenerecombinantmethodswereusedandthegeneenco...
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HBV前C区部分序列亚克隆与FQ-PCR标准曲线的制备
...luorescencequantitativePCRwas0.995.Thelinearrangewasfrom5×103copies/μlto5×109copies/μl.Allthecoefficientofvariationwithinbatchandbetweenbatchweresmallerthan5%.Bothprecisionandrepeatabilityweregood.ConclusionHBVpre-cregiongenestobeusedintheearlydetectionofHepatitisBareprospective.St...
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90μm与130μm角膜瓣LASIK的对比研究
...原位磨镶术准分子激光Contrastanalysisofcornealflapthicknessof90μmand130μminLASIKGUOShi-hong,ZHUYing-fang,CHENMing,etal.DepartmentofOphthalmology,AffiliatedHospitalofXiangnanUniversity,Chenzhou423000,China[Abstract]ObjectiveToanalyzethetherapyandcomplicationofcornealflapthicknessof...
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变形链球菌基因疫苗pcDNA3gtfB鼻黏膜免疫家兔的实验研究
...链球菌;葡糖基转移酶StudyongenevaccinepcDNA3gtfBbyintranasalimmunizationinrabbits WANGJing1LIUJianguo2YANGDeqin2 1DepartmentofOralMediane,AffiliatedHospitalofBinzhouMedicalUniversity,Binzhou256603 2SchoolofStomatology,ZunyiMedicalCollege 【Abstract】ObjectiveToinvest...
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梅毒螺旋体青霉素结合蛋白的克隆及表达
...合成。 1.2.2克隆表达载体的构建Primer1和Primer2各5μl(10pmol/μl),2mMdNTP5μl,10×PCRbuffer10μl,TPDNA10μl,ExpandTMTaq酶2.5U(1μl),无菌无离子水67μl,混匀后快速离心后,加入30μl无菌石蜡油共100μl。同时设置一...
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凯里市2004-2006年学龄儿童尿碘检测结果分析
...的样本进行复检。 1.2.3摄入量估算 按Dunn尿碘100μg/L相当于150μg/d摄入量的方法,以尿碘中位数推算每人每天碘的摄入量。碘摄入量的适宜范围为150~200μg/d,安全范围为100~1000μg/d。 1.2.4判定标准100μg/L(标准...
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双花对人黑素细胞增殖及黑素合成的影响
...育24h,去上清液,每孔加入含一定药物浓度的培养基200μl,双花设6个浓度(0.1μmol/L、1μmol/L、10μmol/L、100μmol/L、400μmol/L、1000μmol/L),每一浓度设4个复孔,对照组直接加培养基200μl,空白孔不加细胞,只加培养基...
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SpotFluorescence法定量DNA
...nsparentPlexiglastrayoverUVTransilluminator(ageltrayisfine).2.Spotseveral3μldropsofTEcontaining2mg/mlEtBrontray.3.Add1μlofaseriesofknownDNAconcentration(lDNAat0,1,2.5,5,1020ng/μl)standardstotheEtBrdrops.Mixbypipettingupanddownseveraltimes.4.Add3dilutions(1/2,1/4,1/8)ofeachofyourunknownDNAsa...
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人正常胃黏膜细胞全长cDNA文库的构建及鉴定
...;cDNA文库;全长ConstructionandidentificationofacDNAlibraryofhumangastricmucosa HEZhen1,XIEYubo2,XIAOQiang1 (FirstAffiliatedHospitalofGuanxiMedicalUniversity,1.DepartmentofGastrointestinalGlandSurgery,2.DepartmentofAnesthesiology,Nanning530021,China) 【Abstract】ObjectiveTocons...
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5-氮杂胞苷对乳腺癌细胞RASSF1A甲基化状态及其基因表达的影响*
...ng,etal.ShenzhenCenterforDiseasePreventionandControl,Shenzhen518020,China5μmol/L),thedemethylationofRASSF1AgeneoccurredandRASSF1AmRNAexpressionwasincressed.ConclusionHymethylationofRASSF1Apromoterwasfoundinhumanbreastcancer.5-AzacytidinemayeffectivelyenhanceRASSF1Agenedemethylationandenhancetheex...
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乙脑病毒鼠源单链抗体的构建及表达
...7株稳定的具有乙脑抗原结合活性的阳性克隆,对其中的mu2H4-3和mu2H4-36进行了序列测定。经分析,2个克隆的基因序列一致,其重链及轻链分别属于Kabat鼠抗体基因家族中第Ⅰ及κ第Ⅵ亚群。构建为融合表达载体pG51-mu2H4后,此单链...
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延边地区500例正常人尿沉渣的参考值调查与分析
...果2.1500例正常人尿液沉渣检测结果(1)尿红细胞:男性0~10/μl,女性0~22/μl,男女性别之间差异有显著意义(P<0.01);(2)白细胞:男性0~12/μl,女性0~27/μl,男女性别之间差异有显著意义(P<0.01);(3)上皮细胞:男性0~9/μl,女性0~19/μl,男女性...
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石墨炉原子吸收法测定人发和血清中痕量钼的研究
...rptionspectrometry. CHENRen-qiang,CAIGang,LIShu-long,etal. (SanyaMunicipalCenterforDiseaseControlandPrevention,Sanya572000,Hainan,P.R.China) Abstract:ObjectiveToestablishanewmethodfordetectionofmolydenuminhumanhairandserum.MethodsCaCl2wasusedasmidifierand1%HNO3asmediatorwithashtempe...
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高效液相色谱法测定心达康片的含量
...thodsThecontentsofquercetin,kaempferolandisofhamnetinweredeterminedbyHPLCsimultaneously.C18column(250×4.6mm,5μm)wasusedwiththemixtureofCH3OH-0.2%phosphate(45∶55)(pH2.27)asthemobilephaseataflowrateof1.0mL·min-1withthedetectionwavelengthat368nm.ResultsThecalibrationcurvesshow...
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氧化苦参碱对细胞共培养体系中酪氨酸酶及黑素的影响
...SO)、四甲基偶氮唑蓝(MTT)、1%抗生素/抗真菌溶液、1μg/ml转移因子、1μg/ml维生素E、5μg/ml胰岛素、0.6ng/ml人碱性成纤维细胞生长因子、10-8mol/Lα-MSH、10-9mol/LET-1购自Sigma公司,M154基础培养基、角质形成细胞生长添...
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DNA定量和质量控制
...sLaboratory1QuantificationandQualityControlofDNAUVQUANTIFICATIONOFDNAAdd15μlofeachsampleto735μlTE,mixwell,andreadOD260andOD280todeterminepurity.RefertotheAppendicesSectionforinstructionsonhowtousetheBeckmanDU-65spectrophotometerandforprogramlistingforautomatedsamplereading.AfterUVquantificatio...
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乙草胺对生物的急性毒理研究
...sforloach werecarriedout.ResultsThesafetyconcentrationofacetochloris1.41μl/L.Loach’sredbloodcellmutationrateintheacetochlorwaterwasmuchhigherthanthatinnormalwater.ConclusionTheherbicideacetochlorcaninducesignificantincreasesinloach’sredbloodcellmutationrate.【Keywords】acetochlor,...
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克氏锥虫的新过氧化物酶基因克隆表达研究
...acutestageofthediseaseandisunsatisfactoryduetotoxicityandlimitedefficacy.Somuchresearcheffortisdirectedatthedevelopmentofnewchemotherapeuticagents. T.cruziisexposedreactiveoxygenspexies(POS)throughoutitslifecycle.ROSssuchasthesuperoxideanion(O-2.)andhydrogenperoxide(H2O2)causecellulardamagedire...
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鞘内不同剂量舒芬太尼在腰硬联合阻滞
...(PCA)。鞘内镇痛药物为舒芬太尼,六组剂量分别为3.0μg、4.0μg、5.0μg、6.0μg、7.0μg、8.0μg;各组病例硬膜外维持用药相同,均为舒芬太尼0.25μg/ml+0.1%罗哌卡因+0.9%NS。观察记录起效时间;鞘内给药后10、15、30、60、90mi...
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关节内注射医用臭氧对大鼠膝退行性关节炎的影响△
...oups:A(groupnormal)、B(groupmodel)、C(groupair),D(groupO2~O335μg/ml)andE(groupO2~O370μg/ml)(n=8ineachgroup).TheOAmodelwasduplicatedbyperformedanterioreruciateligamenttransection(ACLT)andpartialmeniscectomyof1/3inside.AirwasinjectedintojointsofgroupC,accordingtoconcentrati...
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乙型脑炎病毒TaqMan逆转录聚合酶链反应检测方法的建立
...halitisvirus. HUANGFu-xin,GAOShi-tong,ZHANGRen-li,etal. (ShenzhenMunicipalCenterforDiseaseControlandPrevention,Shenzhen518020,Guangdong,P.R.China) Abstract:ObjectiveToestablishaspecificandsensitivemethodfordetectingJapaneseencephalitisvirus(JEV)nucleicacidwithTaqManreversetrascripti...
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糖尿病性黄斑水肿与激光治疗
...以下一项或一项以上:①视网膜水肿增厚在距黄斑中心500μm区域,或小于500μm;②硬性渗出位于距黄斑中心500μm区域,或小于500μm,并伴有邻近视网膜增厚;③视网膜增厚至少有1个视盘直径(diskdiameter,DD)范围,其任何部位病...
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连云港市沿海地区居民碘营养水平调查
...季共检测沿海地区居民的尿样1116份,尿碘中位数为133.4μg/L,尿碘范围为4.2~988.9μg/L,尿碘值小于20μg/L的有14例,占1.25%;尿碘值小于100μg/L的有371例,占33.24%。其中,春季尿样668份,尿碘中位数为114.8μg/L;秋季尿样448份...
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HPLC-ELSD测定灵芪加口服液中黄芪甲苷的含量
...wasusedinthequantitativeanalysisofAstragalosideⅣ.Thecolumnwaspackedwith5μmDiamonsilC_(18)stationaryphase.Themobilephaseconsistedofacetonitrile-water.Thetemperaturewas40℃andthepressurewas3.5Bar.ResultThelinearrangeofthecomponentwas0.864~12.96μg.Theaveragerecoveryratewas99.2%withRSD1.7...