-
国内自身抗体检测的现状及质控分析
...tochondrialantibody,AMA)和抗平滑肌抗体(antismoothmuscleantibody,ASMA)。结果ANA、抗dsDNA抗体、抗ENA抗体、AMA和ASMA的检测正确率分别是86.2%、75.4%、62.7%、100%和61.1%。可见与2002和2003年的质控相比,各项抗体检测的正确率呈上升趋势,但差...
-
异基因造血干细胞移植后自身抗体与cGVHD有关
...体包括抗核抗体(ANA)、抗线粒体抗体(AMA)、抗平滑肌抗体(ASMA)、抗心肌磷脂抗体(ACLA)、抗肝肾微粒体抗体(LKM)、抗DNA抗体、抗中性粒细胞胞质抗体(ANCA)及抗甲状腺抗体,还检测外周血表达抗CD3抗体、CD4抗体、CD8抗体、CD19抗体、CD20抗...
-
IsolationofSidePopulationProgenitorCellsFromHealthyArteriesofAdultMice
...cubationwith10%serumofappropriatespeciesinPBS.Mouseanti--smoothmuscleactin(ASMA)(clone1A4;OncogeneResearchProducts,Boston,Mass),mouseanti-calponin(clonehCP;Sigma),ratanti-CD31(cloneMEC13.3),ratanti-VE-cadherin(clone11D4.1)(BDPharmingen),polyclonalrabbitanti-humanvonWillebrandfactorIg(DAKOCytomation)...
-
CirculatingMatrixMetalloproteinase-9LevelsasaPossibleMarkerofAorticStiffness
...atartificiallyhigherMMP-9concentrationsarefoundinserumsamplescomparedwithplasmasamples.2–5Importantly,althoughahighcorrelationexistsbetweenMMP-9levelsinplasmaseparatedfromblooddrawnintotubeswithdifferentanticoagulants,nocorrelationwasfoundbetweenMMP-9levelsinserumandinplasma.Insummary,preanalytica...
-
第二节 自身抗体的检测及临床意义
...现假阳性。 (三)抗平滑肌抗体 抗平滑肌抗体(ASMA)产生机制不明,但与肝和胆管的自身免疫疾病有关,尤其是慢性活动性肝炎(狼疮性肝炎)和原发性胆汁性肝硬化(PBC)。该类疾病的发生与自身免疫性密切相关,...
-
Isdes-acylghrelincontributingtouremicanorexia?
...rsforhemodialysis-associatedappetitedisorders.Consistentevidenceshowsthatplasmaghrelinconcentrationsareincreasedinadvancedrenalfailure(4)andthatthosehigherconcentrationsdirectlycorrelatewiththeimpairmentofkidneyfunction,whichsuggeststhatreducedrenalexcretionofghrelinisthemaincausativefactorofghrelin...
-
ReplytoDSKalmanandCMColker
...icksonD.Estimationoftheconcentrationoflow-densitylipoproteincholesterolinplasma,withoutuseofthepreparativeultracentrifuge.ClinChem1972;18:499502.SchaeferE,McNamaraJ.Overviewofthediagnosisandtreatmentoflipiddisorders.In:RifaiN,WarnickG,eds.Laboratorymeasurementoflipids,lipoproteinsandapolipopro...
-
Evidence-basedmedicineandvitaminEsupplementation
...yfindingsfromthetrialperiodoftheATBCStudy.Insubjectsinthelowestquintileofplasma-tocopherolconcentration,thesimilarmortalityinthegroupswithsupplementintakesof50and0mg-tocopherol(n=1628and1610,respectively;seeTable3inreference2)refutesthenotionsthatalow-tocopherolintakeie,9.4mg/disthespeci...
-
Disappearanceofeyelidxanthelasmafollowingoralsimvastatin(Zocor)
...cology.comAcceptedforpublication13September2004Keywords:simvastatin;xanthelasmaThemajorriskfactorsforcoronaryheartdiseaseincludesmoking,elevatedbloodpressure,andelevatedserumcholesterol.1Riskreductionstartswithidentificationofthoseatriskandthenalterationoffactorssuchasdiscontinuationofsmoking,loweri...
-
Anewmethodfordeterminingplasmawatercontent:applicationinpseudohyponatremia
...aisaclinicalconditioncharacterizedbyanincreasedfractionofproteinorlipidinplasma,therebyresultinginanartificiallylowplasmasodiumconcentration([Na+]p).Sincetheautomatedmethodofmeasuring[Na+]pinmostlaboratoriesinvolvestheuseofanindirection-selectiveelectrode(I-ISE),thismethoddoesnotcorrectforelevatedpr...
-
Zincdeficiencyinpatientswithsicklecelldisease
...rdiagnosingzincdeficiencyinpatientswithSCDincludeslowzincconcentrationsinplasma,erythrocytes,hair,lymphocytes,andgranulocytesandlowactivitiesofcertainzinc-dependentenzymes,suchascarbonicanhydraseinredbloodcells,alkalinephosphataseinneutrophils,andthymidinekinaseinnewlysynthesizingskinconnectivetissu...
-
大陆地区引用次数最高的生物类论文
...ma,H.,...Experimentalandtheoreticalchallengesinthesearchforthequark-gluonplasma:TheSTARCollaboration‘scriticalassessmentoftheevidencefromRHICcollisions(Cited464time(s))2005;NuclearPhysicsA;Adams,J.,Aggarwal,M.M.,Ahammed,Z.,Amonett,J.,Anderson,B.D.,...Obesityandtheriskofmyocardialinfarctionin27000p...
-
原发性肝癌中自身抗体的检测
... 百分比(%)自身抗体类型占阳性病例 百分比(%)HGG+NNG+ASMAAMAG+N+LMAH+MLMA总计全阴1712.2121000000410.5HBsAg+抗HBe+抗HBc+9870.541431201102668.4抗HBe+抗HBc+128.6120000001410.5抗HBs+抗HBe+抗HBc+42.901000100025.3抗HBs+32.210000000012.6HBsAg+HBeAg+抗HBe+抗HBc+21.40000000...
-
Tacklingthegreatestchallengeincataractsurgery
...precedescapsularbagimplantationofaPMMAIOL.ThetechniqueiscommonlyabbreviatedasmanualSICS(smallincisioncataractsurgery).Anefficient,highvolumesystemutilisinglowcost,sub-5minuteprocedurestotackleadvancedcataractswithminimalcomplicationsisclearlythebestwaytousethescarcestandmostpreciousassetofthesystem...
-
Influenceof10wkofsoyconsumptiononplasmaconcentrationsandexcretionofisoflavonoidsandongutmicroflorametabolisminhealthyadults
...whetherchronicsoyconsumptioninfluencesisoflavonebioavailabilityasjudgedbyplasmaisoflavoneconcentrationsandmodifiedgutmicrofloraactivities[ß-glucosidehydrolysisandequolandO-desmethylangolensin(O-DMA)production].Wealsoexaminedwhethersexdifferencesinisoflavonemetabolismexist.Design:Arandomized,par...
-
Covalently-BoundHeparinMakesCollagenThromboresistant
...entorthroughbypasssurgery.Unfortunately,attemptstoimplantvasculargraftswithasmalldiameterarenotsuccessfulbecauseofthromboticandinflammatoryreactions.1–6Thus,toenhancebloodcompatibility,asurfacehastobedesignedthatisbothanti-immunogenicandthromboresistant.Collagenhasbeenwidelyusedinmedicalapplicatio...
-
UniquePathwayforCholesterolUptakeinFatCells
...eapoE,awell-recognizedapoproteinthatinfluencestheprocessingandclearanceofplasmalipoproteincholesterolandalsoinfluencesintracellularcholesterolhomeostasisinseveralcelltypes.10Inthecurrentstudy,theauthorshavepainstakinglydefinedthemechanismsforthisSR-BI–independentCEselectiveuptakefromHDLinadipocyte...
-
Lipidmetabolismduringenduranceexercise
...ing-inducedincreaseinfatoxidationisdueprimarilytoincreasedoxidationofnon-plasma-derivedfattyacids,perhapsfromintramusculartriacylglycerolstores.Fatoxidationislowerinhigh-intensityexercisethaninmoderate-intensityexercise,inpartbecauseofdecreasedfattyaciddeliverytoexercisingmuscles.Parenterallipidsupp...
-
Lowerplasma-carboxyethyl-hydroxychromanafterdeuterium-labeled-tocopherolsupplementationsuggestsdecreasedvitaminEmetabolisminsmokers
...othesizedthatsmokerswouldhaveless-tocopherolavailableandconsequentlylowerplasma-carboxyethyl-hydroxychroman(-CEHC),the-tocopherolmetaboliteproducedbyacytochromeP450mediatedprocess.Design:Smokersandnonsmokers(n=10pergroup)weresupplementedwithdeuterium-labeled-tocopherylacetates(75mgeachd3-RRR--...
-
男性不育患者免疫因素AsAb与TNF-α的相关性研究
...,Wuhu241001.【Abstract】ObjectiveTostudytherelationshipbetweenseminalplasmaandserumofTNF-inmaleinfertilˉity.MethodswedetectedTNF-αconcentrationsinseminalandserumplasmathroughradioimmunoassay;anddeˉtectedAsAbinseminalandserumplasamathroughELISA.Results(1)TheTNF-αconcentrationsinseminalpla...
-
Smokingandexposuretoenvironmentaltobaccosmokedecreasesomeplasmaantioxidantsandincrease-tocopherolinvivoafteradjustmentfordietaryantioxidan
...etomacromolecules,contributingtocardiovasculardiseasesandcancer.Decreasedplasmaantioxidantconcentrationsmayindicatecigarettesmokerelatedoxidativestress.Objective:Wecomparedtheeffectsonplasmaantioxidantconcentrationsincotinine-confirmedactiveandpassivesmokerswiththoseinnonsmokers,independentofd...
-
AdenoviralTransferofEndothelialNitricOxideSynthaseAttenuatesLesionFormationinaNovelMurineModelofPostangioplastyRestenosis
...olesterolemia,tendstobehypercellular,highlyenrichedinsmoothmusclecellactin(ASMA)content,andrelativelydevoidofextracellularmatrixcomponents.13–15Inclinicalpractice,however,atheroscleroticlesionsformthelipid-richandinflammatory“soil“ofrestenosis,whichisreflectedhistologicallybythepresenceoffoamc...
-
Atrialnatriureticpeptide:anessentialphysiologicalregulatoroftransvascularfluid,proteintransport,andplasmavolume
...s,California,USA.AbstractAtrialnatriureticpeptide(ANP)actsacutelytoreduceplasmavolumebyatleast3mechanisms:increasedrenalexcretionofsaltandwater,vasodilation,andincreasedvascularpermeability.AuthorsofastudyinthisissueoftheJCIperformedaknockoutofthereceptorforANPinvascularendotheliainordertodistinguis...
-
BiologicallyActiveCD40LigandIsElevatedinSickleCellAnemia
...entiallyplaysaroleinSCA.MethodsandResults-ELISAsdemonstratethatSCApatientplasmacontains30-foldmoresCD40Lthancontrolplasma.Correspondingly,plateletsfromthesepatientscontainlessthanhalftheCD40Lfoundincontrolplatelets.PlateletsfrompatientsinpainfulcrisesarefurtherdepletedofCD40L,withevenhigherplasmalev...
-
不孕妇女β-内啡肽与生殖激素水平及其与精神心理因素关系的探讨
...sweremeasured,andtheovulationmonitoredsimultraneously.Results(1)Theplasmalevelofβ-EPininfertilegroupandcontrolwerelowestinmid-follicularphase,elevatedalittleinperiovulatoryphaseandraisedsignificantlyinmid-lutealphase(P0.05),displayingaascendingtrendincycles.Ininfertilegroupplasmaβ-EP...
-
S-Adenosylhomocysteine—abetterindicatorofvasculardiseasethanhomocysteine?
...il:conrad.wagner{at}vanderbilt.edu.ABSTRACTItiswidelyacceptedthatelevatedplasmatotalhomocysteineisanindependentriskfactorforvasculardisease.Therelationisbelievedtobecausal,butthereisnogenerallyacceptedmechanismforthepathophysiologyinvolved.ThemetabolicprecursorofhomocysteineinalltissuesisS-adenosylh...
-
Two-photoninvivomicroscopyofsulfonefluoresceinsecretioninnormalandcysticratkidneys
...an‘scapsuleaveraged0.15±0.04(n=17)timesthatofglomerularcapillaryplasma,consistentwithextensiveplasmaproteinbindingofSF.InnormalHan:SPRDrats,steady-statecellcytoplasmSFfluorescenceinproximaltubuleanddistaltubulecellsaveraged,respectively,2.7±1.4(n=99tubules)and0.2±0.2(n=17)time...
-
老年人冠心病发病与血浆同型半胱氨酸水平相关性研究
...。【参考文献】1UelandPM,RefsumH,StablerSP,etal.Totalhomocysteineinplasmaofserum:Methodsandclinicalapplications.ClinChem,1993,39(9):1764-1769.2JacobsenDW,GatautisVJ,GreenR,etal.RapidHPLCdeterminationoftatalhomocysteineandotherthiofinserumandplasma:sexdifferentandcorrelationwithcobafaminand...
-
IdentificationandCharacterizationofClinicalIsolatesofMembersoftheStaphylococcussciuriGroup
...2strains).Staphylocoagulase(freecoagulase)activitywasdeterminedwithrabbitplasma(Torlak,Belgrade,Serbia)byusingthetubemethod(5).Oxidaseactivitywasdeterminedwithoxidasediagnostictablets(Rosco,Taastrup,Denmark).NovobiocinsusceptibilitywasdeterminedonMueller-Hintonagar(OxoidLimited,Basingstoke,Hampshire...
-
第四章 受体的术前评估和治疗--第一节 受体的术前评估
...抗体(ANA);④抗线粒体抗体(AMA);⑤抗平滑肌抗体(ASMA);⑥EB病毒抗体;⑦巨细胞病毒(CMV)抗体;⑧甲胎蛋白(AFP)和癌胚抗原(CEA);⑨人类免疫缺陷病毒(HIV)。 对于Budd-Chiari综合征、酒精性肝硬化及其他原因...