CellCycle–DependentRegulationofSmoothMuscleCellActivation
...peroxidasecomplex.Antibodybindingwasvisualizedwith3-amino-9-ethylcarbazole(AEC;Zymed).Cellswerecounterstainedwithhematoxylin.OmissionofprimaryantibodiesandthestainingwithmousenonimmuneIgGservedasanegativecontrol.FlowCytometryCellswereharvestedbytrypsinization,fixedovernightwith75%methanol,washed,and...
医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2004年第24卷第5期A20-YearProspectiveCommunityStudy
...ildhoodanxiety,andafamilyhistoryofallergywereimportantconfoundersoftheasthmaeCpanicassociation.Conclusions:Thisisthefirstlong-termfollow-upstudyonasthmaandpanic.ItshoweddoseeCresponse-typerelationshipsbetweenpanicandasthma,andbidirectionallongitudinalassociationsbetweenthetwoconditions.Itprovidedevi...
医源资料库;在线期刊;美国呼吸和危急护理医学;2005年第171卷第6期SynergisticProductionofLungFreeRadicalsbyDieselExhaustParticlesandEndotoxin
...httobethebasisofXDH/XOinvolvementinvariouspathologicconditionssuchasischemiaeCreperfusioninjury(40,41),influenzavirusinfection(42),andneutrophil-mediatedlunginjury(43).Inthepresentstudy,wefoundthattheintratrachealcoadministrationofDEPandLPSsynergisticallyenhancedfreeradicalsinthelung,primarilyinthef...
医源资料库;在线期刊;美国呼吸和危急护理医学;2005年第171卷第2期Interdependencyof-AdrenergicReceptorsandCFTRinRegulationofAlveolarActiveNa+Transport
...ialsandMethodsAdenovirusPropagationandPurificationReplication-incompetentE1aeC/E3eChumantype5adenovirusescontainingeitherafull-lengthhumanCFTRcDNAunderthecontrolofahumanCMVimmediate-earlypromoterenhancergene(adCFTR,fromDrsM.WelshandJ.Zabner,UniversityofIowa,IowaCity,Iowa),ahuman2ARcDNAunderthecontro...
医源资料库;在线期刊;循环研究杂志;2005年第95卷第5期LeukocyteTransglutaminase2ExpressionLimitsAtheroscleroticLesionSize
...our;Vectastain(Vector)for30minutesanddevelopedwith3-amino-9-ethylcarbazole(AEC;Vector).ForimmunohistochemicalanalysisoflesionalTG2andanti-N(-glutamyl)lysinecross-links,acetone-fixedfrozensectionswereblockedandstainedusingtheHistoMouse-kit(ZymedLaboratories).DetectionofperoxidaseusedAEC.Controlsectio...
医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2006年第26卷第3期RegulationofEndopeptidasesEC3.4.24.15andEC3.4.24.16inVascularEndothelialCellsbyCyclicStrain:RoleofGiProteinSignaling
...sionandenzymaticactivityofEP24.15andEP24.16inbovineaorticendothelialcells(BAECs).MethodsandResults—BAECswereculturedunderconditionsofdefinedcyclicstrain(0%to10%stretch,60cycles/min,0to24hours).StrainsignificantlyincreasedEP24.15andEP24.16solubleactivityinaforce-andtime-dependentmanner,withelevatio...
医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2004年第24卷第3期HighPrevalenceofCirculatingCD4+CD28–T-CellsinPatientsWithSmallAbdominalAorticAneurysms
...mplex(SABC;DAKOCytomation).Theenzymewasdetectedby3-amino-9-ethyl-carbazole(AEC;Sigma)yieldingabrownish-reddishcolor.Sectionswerecounter-stainedwithhematoxylin.Fordoublestaining,donkeyanti-mouseboundalkalinephosphatasewasdevelopedwithfastblueBBsalt(Sigma)andHRPwithAEC.Formoredetailspleaseseetheexpand...
医源资料库;在线期刊;动脉硬化血栓血管生物学杂志;2005年第25卷第7期FasLigand-DependentInflammatoryRegulationinAcuteMyocarditisInducedbyTrypanosomacruziInfection
...peroxidase(Sigma)in1-hourincubationandrevealedwith3-amino-9-ethylcarbazole(AEC;Sigma)asdescribedelsewhere.26Directquantificationofmastcellswasperformedbytoluidinebluestainingincardiacslices16-µmthicktoconfirmfluorescence-activatedcellsortinganalysis(seebelow).InflammatoryCellHarvestingHeartsfr...
医源资料库;在线期刊;美国病理学杂志;2007年第169卷第7期ChlamydophilapneumoniaeandMycoplasmapneumoniae
...ma,andacuteasthmaexacerbations.CHLAMYDOPHILAPNEUMONIAEANDMYCOPLASMAPNEUMONIAEC.pneumoniaeisaubiquitousobligateintracellularbacterium,whichisentirelydependentonenergyproducedbythehostforitsreplicationwithinthehostcellcytoplasm,wherethebacteriaformcharacteristicintracellularinclusions(6).C.pneumoniaei...
医源资料库;在线期刊;美国呼吸和危急护理医学;2005年第171卷第11期Controloftightjunctionalsealing:roleofepidermalgrowthfactor
...fcanineoxynticmucosalcells(15),LLC-PK1cells(51,57),alveolarepithelialcells(AEC)(5),andMDCK-IIcells(55).InAEC,EGFstimulatestheexpressionofcln-4and-7anddownregulatescln-3and-5(16).SinghandHarris(55)reportedthatEGFtreatmentofMDCK-IIcellsincrementsboththeexpressionandlocalizationtotheTJofcln-1,-3,and-4,...
医源资料库;在线期刊;美国生理学杂志;2007年第290卷第2期