OntheMechanismofActionof9-O-ArylalkyloximeDerivativesof6-O-Mycaminosyltylonolide,aNewClassof16-MemberedMacrolideAntibiotics
...pendentpoly(Phe)synthesis.However,theyseverelyinhibitpoly(A)-dependentpoly(Lys)synthesisandcompetewitherythromycinortylosinforbindingtocommonorpartiallyoverlappingsitesintheribosome.Accordingtofootprintinganalysis,thelactoneringofthesecompoundsseemstooccupytheclassicbindingsiteofmacrolidesthatisloca...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第10期tuftsin及其抑制物的化学合成和对巨噬细胞抗胆管癌作用的影响
...c方案,起始采用0.0125mmol/L树脂酸(美国ABI公司),按照Thr-Lys-Pro-Arg的序列使肽链从C端逐个向N端延伸。各种氨基酸的保护基团是:各氨基酸的α-氨基为Fmoc保护,其它侧链保护基团为Thy(TrT),Lys(Boc),Arg(PMC)。每步缩合都加入HoBt/Dcc...
合作平台;医学论文;临床医学与专科论文;检验医学MastoparanChangestheCellularLocalizationofGq/11andGthroughItsBindingtoGangliosideinLipidRafts
...e,wehavedemonstratedpreviouslythatmastoparansuppressedphosphoinositidehydrolysisinducedbycarbacholinhumanastrocytomacells(FEBSLett206:91–94,1990).Inthepresentstudy,weexaminedwhethermastoparanaffectedphosphoinositidehydrolysisbyinteractingwithlipidraftsinPC-12cells.MastoparaninhibitedUTP-inducedinc...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第5期RoleofNuclearFactor-BandProteinKinaseCSignalingintheExpressionoftheKininB1ReceptorinHumanVascularSmoothMuscleCells
...A)]allincreasedthedensityofbindingsitesforthetritiatedB1receptoragonist[3H]Lys-des-Arg9-bradykinin(withoutchangeinreceptoraffinity)incell-basedassays.SmallinterferingRNAassaysindicatedthatNF-Bp65isnecessaryfortheeffectiveexpressionofthecellsurfaceB1receptorunderbasalorIL-1,fetalbovineserum(FBS),orPM...
医源资料库;在线期刊;分子药理学杂志;2007年第69卷第3期刘勇军教授Nature子刊免疫学新文章
...SPRY-PRY结构域与DDX41的DEADc结构域发生了相互作用。DDX41的Lys9和Lys115位点是TRIM21介导的泛素化作用的靶点。这些研究结果表明TRIM21是一种干扰素诱导的E3连接酶,其可诱导DDX41Lys48(K48)关联的泛素化及降解,负调控了对于细胞内dsDNA...
行业资讯;临床快报;免疫系统XPD/ERCC2多态性与晚期结直肠癌对奥沙利铂敏感性关系
【摘要】目的了解XPD/ERCC2Lys751Gln(C→A,35931)多态性与晚期结直肠癌对奥沙利铂为主化疗敏感性的关系。方法70例Ⅳ期结直肠癌病人化疗前取静脉血并提取DNA,以real-timePCR法对XPD/ERCC2基因进行单核苷酸多态性(SNP)分型。对病人行...
医源资料库;在线期刊;齐鲁医学杂志;2009年第24卷第3期MolecularDeterminantsoftheAgonistBindingDomainofaP2XReceptorChannel
...dues.Here,weusedtheknownsequenceandsecondarystructuresimilaritiesbetweentheLys180-Lys326ectodomainregionofP2X4andtheclassIIaminoacyl-tRNAsynthetasesasaguidetogenerateathree-dimensionalmodelofthereceptor-bindingsiteandtodesignmutants.Theinterplaybetweenhomologymodelingandsite-directedmutagenesissugge...
医源资料库;在线期刊;分子药理学杂志;2005年第67卷第4期RoleofEctodomainLysinesintheSubunitsoftheHeteromericP2X2/3Receptor
【关键词】ectodomainLysineresiduesneareachendofthereceptorectodomain(inratP2X2Lys69andLys308)havebeenimplicatedinATPbindingtoP2Xreceptors.WerecordedmembranecurrentsfromhumanembryonickidneycellsexpressingP2Xsubunitsandfoundthatlysine-to-alaninesubstitutionsatequivalentpositionsintheP2X3receptor(...
医源资料库;在线期刊;分子药理学杂志;2006年第68卷第10期ShortPolybasicPeptideSequencesArePotentInhibitorsofPC5/6andPC7:UseofPositionalScanning-SyntheticPeptideCombinatorialLibrariesasaToolfort
.../6AandPC7.TheL-configurationlibrarysurveysdistinctivelyrevealedthatL-Arg,L-Lys,andsometimesL-Hisinallsixpositionswouldgeneratethemostpotentinhibitorsforbothenzymes.Basedonthisclearpolybasicpreference,L-poly-Argpeptidesrangingfromfourtonineresidueswereassayed.Inhibitorypotencyofthesepolybasicpeptides...
医源资料库;在线期刊;分子药理学杂志;2007年第69卷第1期Mitochondrialaldehydedehydrogenase-2(ALDH2)Glu504Lyspolymorphismcontributestothevariationinefficacyofsublingualnitroglycerin
...y,weshowthatthecommonG-to-Apolymorphisminexon12ofALDH2—resultinginaGlu504LysreplacementthatvirtuallyeliminatesALDH2activityinbothheterozygotesandhomozygotes—isassociatedwithalackofefficacyofsublingualGTNinChinesesubjects.Wealsoshowthatthecatalyticefficiency(Vmax/Km)ofGTNmetabolismoftheGlu504prot...
医源资料库;在线期刊;临床研究杂志;2006年第116卷第2期