-
抗纤复方及TGF-β对胶原基因启动子活性的影响①
...pCOL0.8重组体构建 含小鼠α2(Ⅰ)胶原-2kb~+54bp序列的质粒pAZ1009由美国NICCrombrugghe教授惠赠(参见图1)[6],重新构建的重组体所用载体为不含启动子,但含有SV40增强子、氯霉素乙酰基转移酶(CAT)报告基因的质粒pCAT-enhancer(promega...
-
RNA干扰作用原理及其应用
...断(siRNA)[5]。Dicer含有解旋酶活性以及dsRNA结合域和PAZ结构。在RNAi的效应阶段,siRNA双链结构解旋并形成有活性的蛋白/RNA复合物(RNA-inducedsilencingcomplexorRISC),在siRNA解双链即RISC激活过程需一个ATP[5]。由RISC中siRNA反义链与...
-
细胞因子对小鼠α2(I)型前胶原基因启动子活性影响的研究①
...,将它与氯霉素乙酰基转移酶(CAT)报告基因组成的重组体pAZ1009用脂质体法转染至胶原产生细胞NIH/3T3细胞,讨论IFNα、IFNγ、TNFα对小鼠α2(I)型前胶原基因上游启动子活性的影响。结果:TNFα抑制小鼠α2(I)型前胶原基因启动子活性...
-
DoesResistancetoPyrazinamideAccuratelyIndicatethePresenceofMycobacteriumbovis
...A.Hahn,P.M.Small,C.L.Daley,M.A.Behr,A.R.Moss,J.M.Creasman,G.F.Schecter,E.A.Paz,andP.C.Hopewell.1999.AmolecularepidemiologicanalysisoftuberculosistrendsinSanFrancisco,1991-1997.Ann.Intern.Med.130:971-978.Kamerbeek,J.,L.Schouls,A.Kolk,M.vanAgterveld,D.vanSoolingen,S.Kuijper,A.Bunschoten,H.Molhuizen,R....
-
SensitivitiesandSpecificitiesofSpoligotypingandMycobacterialInterspersedRepetitiveUnit-Variable-NumberTandemRepeatTypingMethodsforStudyingMole
...epidemiologicmethods.N.Engl.J.Med.330:1710-1716.Behr,M.A.,P.C.Hopewell,E.A.Paz,L.M.Kawamura,G.F.Schecter,andP.M.Small.1998.PredictivevalueofcontactinvestigationforidentifyingrecenttransmissionofMycobacteriumtuberculosis.Am.J.Respir.Crit.CareMed.158:465-469.Daley,C.L.,P.M.Small,G.F.Schecter,G.K.Schoo...
-
RNA干涉过程中“小干涉RNA”的识别
...特定信使RNA进行降解的指导要素。现在,结构研究确定,PAZ(Argonaute蛋白家族特有的一个区域,该家族的蛋白与RNA沉寂机制有关)专门识别siRNA,方法是通过将siRNA的3个悬臂固定。作者:
-
RNA诱导沉默复合体中的生物大分子及其装配
...结构和功能的作用;Argonaute蛋白是RISC中的核心蛋白,有PAZ和PIWI两个主要的结构域,前者为siRNA的传递提供结合位点,后者是RISC中的酶切割活性中心;siRNA是RISC完成特异性切割作用的向导,在成熟的RISC中虽然只包含siRNA的一条链...
-
牵牛花和RNA干扰
...basemadeupoftheamino-terminal,middle,andPIWIdomains.ThePiwiArgonauteZwille(PAZ)domainisheldabovethebasebyastalk-likeregion.ThePIWIdomain(namedfortheproteinpiwi)issimilartoribonucleaseH,withaconservedactivesiteaspartate-aspartate-glutamatemotif,stronglyimplicatingArgonauteasSlicer.Thearchitectureofth...
-
研究发现抑制RNAi抗病毒先天免疫新机制
...制蛋白B2的研究发现,B2可以通过直接作用于果蝇Dicer-2的PAZ及RNaseIII结构域,从siRNA产生及RNA沉默复合体(RISC)组装两个层面抑制Dicer-2蛋白在细胞中的功能。这是第一次证明了宿主Dicer蛋白可以直接被病毒抑制,并详细的阐释了...
-
噬菌体鸡尾酒拯救葡萄
...,根据生理和遗传性质将其分为四种,Sano、Salvo、Prado和Paz,它们各不相同。”课题组成员TusharSuvraBhowmick博士说道。生长在温室里的葡萄接种了细菌和四种噬菌体鸡尾酒制剂。实验团队既试验了植物感染叶缘焦枯病菌后供应其噬...
-
Anthropometricmeasuresinmiddleageafterexposuretofamineduringgestation:evidencefromtheDutchfamine
...ta,GA(HSK);theMailmanSchoolofPublicHealth,ColumbiaUniversity,NewYork,NY(AR,PAZ,andLHL);andTNOQualityofLife,Leiden,Netherlands(KvdP)2ThefindingsandconclusionsinthisreportarethoseoftheauthorsanddonotnecessarilyrepresenttheviewsoftheCentersforDiseaseControlandPrevention.3SupportedbygrantRO1HL067914(Pri...
-
Long-TermMolecularAnalysisofTuberculosisStrainsinAlabama,aStateCharacterizedbyaLargelyIndigenous,Low-RiskPopulation
...iemer,P.M.Small,A.P.deLeon,R.Steinhart,G.F.Schecter,C.L.Daley,A.R.Moss,E.A.Paz,R.M.Jasmer,C.B.Agasino,andP.C.Hopewell.1998.DifferencesincontributingfactorstotuberculosisincidenceinU.S.-bornandforeign-bornpersons.Am.J.Respir.Crit.CareMed.158:1797-1803.Cronin,W.A.,J.E.Golub,M.J.Lathan,L.N.Mukasa,N.Hoo...
-
RNA干扰技术及其在泌尿系肿瘤治疗中的应用
...eotide)的siRNA(smallinterferenceRNA)双链。②效应阶段:Dicer通过PAZ结构域与含有PAZ结构域的Argonaute蛋白互相作用,核酸内外切酶、解旋酶与Argonaute蛋白相连,进而与siRNA一起形成具有多个亚单元的核糖核苷酸蛋白复合物(RNAinducingsilencingc...
-
胸腔镜术后发生肺漏气的护理
...CardiovascSurg,1991,102:721.4CuschieriA,BerciG,Paz-PartlowM,etal.Laparoscopicbiliarysurgery,2nded.Oxford,Blackwellscientificpublications,1991,51-55.5孙静.电视胸腔静微创手术治疗肺大泡护理体会.蚌埠医学院学报,2003,28(2):648.作者单位:21406...
-
ASCO2015带给我的10大临床决策改变
...常昂贵。2.推荐Nivolumab用于难治性晚期非鳞非小细胞肺癌Paz-Ares等[2]在ASCO上报道,一项全球多中心III期临床研究发现,将接受铂类为基础的双药方案进展的晚期非鳞非小细胞肺癌(NSCLC)患者随机分为Nivolumab组或多西他赛组,接...
-
VascularEndothelialGrowthFactorIsInducedbytheInflammatoryCytokinesInterleukin-6andOncostatinMinHumanAdiposeTissueInVitroandinMurineAdip
...ndvisceralwhiteadiposetissueandinthehumanbrownadiposetissuederivedcell-linePAZ6.FurthermorewedeterminedpossibleeffectsofIL-6andOSMontheexpressionofVEGFinadiposetissueinmiceinvivo.MaterialsandMethodsPrimaryculturesofhumanvisceralandsubcutaneouspreadipocytes,adipocytes,andexvivocultureswerepreparedfro...
-
MycobacteriummarinumInfectionsinFishandHumansinIsrael
...erizationofagenecodingfor16SribosomalRNA.NucleicAcidsRes.17:7843-7853.Even-Paz,Z.,H.Haas,T.Sacks,andE.Rosenmann.1976.Mycobacteriummarinumskininfectionsmimickingcutaneousleishmaniasis.Br.J.Dermatol.94:435-442.Falkinham,J.O.1996.Epidemiologyofinfectionbynontuberculousmycobacteria.Clin.Microbiol.Rev.9:...
-
PresenceofDNAofHumanPapillomavirus16butNoOtherTypesinTumor-FreeTonsillarTissue
...lomavirustype16DNAincarcinomasofthepalatinetonsil.J.Clin.Pathol.43:918-921.Paz,I.B.,N.Cook,T.Odom-Maryon,Y.Xie,andS.P.Wilczynski.1997.Humanpapillomavirus(HPV)inheadandneckcancer.AnassociationofHPV16withsquamouscellcarcinomaofWaldeyer‘stonsillarring.Cancer79:595-604.Resnick,R.M.,M.T.Cornelissen,D.K...
-
EndophthalmitisCausedbyFusariumproliferatum
...ology,andclinicalaspectsofFusariumspecies.Clin.Microbiol.Rev.7:479-504.Nir-Paz,R.,J.Strahilevitz,M.Shapiro,N.Keller,A.Goldschmied-Reouven,O.Yarden,C.Block,andI.Polacheck.2004.ClinicalandepidemiologicalaspectsofinfectionscausedbyFusariumspecies:acollaborativestudyfromIsrael.J.Clin.Microbiol.42:3456-3...
-
AssociationsofPPARGC1AHaplotypesWithPlaqueScorebutNotWithIntima-MediaThicknessofCarotidArteriesinMiddle-AgedSubjects
...indingsiteandaffectsPGC-1expressioninINS1-EcellsbuthaslittleeffectinHepG2orPAZ6cells.6PreliminarystudiesinTHP-1orhumanumbilicalveinendothelialcellsrevealedminoreffectsofthisSNPontheexpressionofareportergeneunderbasalcultureconditions(datanotshown).However,phasedgenotypingin46allelesshowedcompletelin...
-
EpidemiologicImportofTuberculosisCasesWhoseIsolatesHaveSimilarbutNotIdenticalIS6110RestrictionFragmentLengthPolymorphismPatterns
...berculosis.Theor.Popul.Biol.63:347-363.Small,P.M.,P.C.Hopewell,S.P.Singh,A.Paz,J.Parsonnet,D.C.Ruston,G.F.Schecter,C.L.Daley,andG.K.Schoolnik.1994.TheepidemiologyoftuberculosisinSanFrancisco.Apopulation-basedstudyusingconventionalandmolecularmethods.N.Engl.J.Med.330:1703-1709.Thierry,D.,M.D.Cave,K.D...
-
MolecularFingerprintingofLeishmaniainfantumStrainsfollowinganOutbreakofVisceralLeishmaniasisinCentralIsrael
...orthernMinnesotabeaverponds.Proc.Natl.Acad.Sci.USA92:5001-5005.Ephros,M.,A.Paz,andC.L.Jaffe.1994.AsymptomaticvisceralleishmaniasisinIsrael.Trans.R.Soc.Trop.Med.Hyg.88:651-652.Gdalevich,M.,D.Mimouni,I.Ashkenazi,andJ.Shemer.2000.RabiesinIsrael:decadesofpreventionandahumancase.PublicHealth114:484-487.G...
-
DendriticcellsstarinVancouver
...indendriticcells.Nature.425:397–402.Zuniga,E.I.,D.B.McGavern,J.L.Pruneda-Paz,C.Teng,andM.B.Oldstone.2004.Bonemarrowplasmacytoiddendriticcellscandifferentiateintomyeloiddendriticcellsuponvirusinfection.Nat.Immunol.5:1227–1234.Lindquist,R.L.,G.Shakhar,D.Dudziak,H.Wardemann,T.Eisenreich,M.L.Dustin,...
-
Flow-MediatedDilatationIsImpairedbyaHigh–SaturatedFatDietbutNotbyaHigh-CarbohydrateDiet
...lCardiol.1999;33:805–812.FuentesF,Lopez-MirandaJ,SanchezE,SanchezF,PaezJ,Paz-RojasE,MarinC,GomezP,Jimenez-PereperezJ,OrdovasJM,Perez-JimenezF.Mediterraneanandlow-fatdietsimproveendothelialfunctioninhypercholesterolemicmen.AnnInternMed.2001;134:1115–1119.deRoosNM,BotsML,SiebelinkE,SchoutenE,Katan...
-
DiabeticMicroangiopathyinIschemicLimbIsaDiseaseofDisturbanceofthePlatelet-DerivedGrowthFactor-BB/ProteinKinaseCAxisbutNotofImpairedExpre
...etes.2002;51:1556eC1564.Guzman-GardearzabalE,Leyva-BohorquezG,Salas-ColinS,Paz-JaneiroJL,Alvarado-RuizR,Garcia-SalazarR.Treatmentofchroniculcersinthelowerextremitieswithtopicalbecaplermingel.01%:amulticenteropen-labelstudy.AdvTher.2000;17:184eC189.RivardA,SilverM,ChenD,KearneyM,MagnerM,AnnexB,Peters...
-
AssociationsoftheUCP2GeneLocusWithAsymptomaticCarotidAtherosclerosisinMiddle-AgedWomen
...expression,increasedtranscriptionofareportergeneinthehumanadipocytecelllinePAZ6,anddecreasedriskofobesityinmiddle-agedhumans.12Ina?-cellenvironment,thevariantallelewasmoreeffectivelyactivatedbythetranscriptionfactorPAX6andwasassociatedwithareduceddispositionindex(theproductoftheacuteinsulinresponset...
-
ReducedmitochondrialdensityandincreasedIRS-1serinephosphorylationinmuscleofinsulin-resistantoffspringoftype2diabeticparents
...ate-1tyrosinephosphorylationrequiresserine612.Biochemistry.36::12939-12947.Paz,K.etal.1999..Phosphorylationofinsulinreceptorsubstrate-1(IRS-1)byproteinkinaseBpositivelyregulatesIRS-1function.J.Biol.Chem.274::28816-28822.Hirosumi,J.etal.2002..AcentralroleforJNKinobesityandinsulinresistance.Nature.420...
-
MolecularTypingofMycobacteriumtuberculosisbyMycobacterialInterspersedRepetitiveUnit-Variable-NumberTandemRepeatAnalysis,aMoreAccurateMethodfo
...l.39:69-74.Geng,E.,B.Kreiswirth,C.Driver,J.Li,J.Burzynski,P.DellaLatta,A.LaPaz,andN.W.Schluger.2002.ChangesinthetransmissionoftuberculosisinNewYorkCityfrom1990to1999.N.Engl.J.Med.346:1453-1458.Hawkey,P.M.,E.G.Smith,J.T.Evans,P.Monk,G.Bryan,H.H.Mohammed,M.Bardhan,andR.N.Pugh.2003.Mycobacterialintersp...
-
LongQTsyndrome:fromchannelstocardiacarrhythmias
...ogicalandtemperatureeffects.J.Biol.Chem.274::31123-31126.Ficker,E.,Obejero-Paz,C.A.,Zhao,S.,andBrown,A.M.2002..ThebindingsiteforchannelblockersthatrescuemisprocessedhumanlongQTsyndrometype2ether-a-gogo-relatedgene(HERG)mutations.J.Biol.Chem.277::4989-4998.Paulussen,A.etal.2002..Anovelmutation(T65P)i...
-
MProteinGene(emmType)AnalysisofGroupABeta-HemolyticStreptococcifromEthiopiaRevealsUniquePatterns
...-Goor,J.Jaffe,I.Shetzigovsky,M.Ravins,Z.Korenman,R.Cohen-Poradosu,andR.Nir-Paz.2003.emmTypingofMnontypeableinvasivegroupAstreptococcalisolatesinIsrael.J.Clin.Microbiol.41:4655-4659.Pruksakorn,S.,N.Sittisombut,C.Phornphutkul,C.Pruksachatkunakorn,M.F.Good,andE.Brandt.2000.Epidemiologicalanalysisofnon-...